B0JN99 (SYN_MICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	MAE_04410

Organism

Microcystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]

Taxonomic identifier

449447 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Microcystis

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000081851

Sequences

	Sequence		Length	Mass (Da)
	B0JN99 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 81C4CCD80AE6287B	FASTA	454	51,586
	10 20 30 40 50 60 MTTRIKEIFQ TGQPDQSVTV QGWVRTKREL KEFTFLEVND GSSLANLQVI LEPTLPDYEN 70 80 90 100 110 120 VLKTISTGAA IAVSGNLVPS PGKGQNIELK AAEITLYGDC PPDYPLQKKR HSFEFLRTIA 130 140 150 160 170 180 HLRARTNTLG AVMRVRNACA TAIHSFFQEK GFIWVHTPII TANDCEGAGE LFTVTSLDLK 190 200 210 220 230 240 KPANFAEDFF GKRAYLTVSG QLQAEVMAMA LSNVYTFGPT FRAENSNTSR HLAEFWMVEP 250 260 270 280 290 300 EMAFCDLEGD QDLAEAFLKY IFKFVLENCP EDLQFFNERI DKTVLSTAEN IVNSEFGRIT 310 320 330 340 350 360 YSEAIELLEK ADRQFEFPVE WGVDLQSEHE RYLAEELFKK PVIVTNYPKT IKAFYMRLDD 370 380 390 400 410 420 NNKTVSAMDI LAPKIGEIIG GSQREERLDV LIQRMQEQGM NPDDLWWYLD LRRYGSVPHA 430 440 450 GFGLGFERLV QFMTGMTNIR DVIPFPRTPL SADF

References

[1]

"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843." Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M. DNA Res. 14:247-256(2007) [PubMed: 18192279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIES-843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009552 Genomic DNA. Translation: BAG00263.1.
RefSeq	YP_001655455.1. NC_010296.1.
3D structure databases
ProteinModelPortal	B0JN99.
ModBase	Search...
Protein-protein interaction databases
STRING	B0JN99.
Proteomic databases
PRIDE	B0JN99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5861077.
GenomeReviews	Gene locus MAE_04410 in contig AP009552_GR.
KEGG	mar:MAE_04410.
PATRIC	22626151. VBIMicAer59304_0416.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	B0JN99.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	MAER449447:MAE_04410-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_MICAN

Accession

Primary (citable) accession number: B0JN99

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program