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B0JJ58

- DAPF_MICAN

UniProt

B0JJ58 - DAPF_MICAN

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Microcystis aeruginosa (strain NIES-843)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei47 – 471SubstrateUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Active sitei75 – 751Proton donor/acceptorUniRule annotation
    Binding sitei164 – 1641SubstrateUniRule annotation
    Sitei166 – 1661Important for catalytic activityUniRule annotation
    Binding sitei197 – 1971SubstrateUniRule annotation
    Sitei215 – 2151Important for catalytic activityUniRule annotation
    Active sitei224 – 2241Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciMAER449447:GHO8-5992-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:MAE_59440
    OrganismiMicrocystis aeruginosa (strain NIES-843)
    Taxonomic identifieri449447 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis
    ProteomesiUP000001510: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Diaminopimelate epimerasePRO_1000077699Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 224

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiB0JJ58.
    PRIDEiB0JJ58.

    Interactioni

    Protein-protein interaction databases

    STRINGi449447.MAE_59440.

    Structurei

    3D structure databases

    ProteinModelPortaliB0JJ58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 112Substrate bindingUniRule annotation
    Regioni75 – 773Substrate bindingUniRule annotation
    Regioni215 – 2162Substrate bindingUniRule annotation
    Regioni225 – 2262Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220467.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0JJ58-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIPFTKYQG LGNDFILIDN RHSPEPLITA EMAVAMCDRH FGIGADGVIF    50
    ALPGQAETDY TMRIFNSDGS EPEMCGNGIR CLAQFIARLE ANNAIGRTYR 100
    IHTLAGTIIP RLEANEQVTV DMGPPQLLGS EIPTTLVKGS EKVLAVPLEV 150
    EGKDWLVTCV SMGNPHCVTF VGDLASIPLE TIGPKFEHHP VFPQRTNVEF 200
    VEVIAPDYMK MRVWERGAGI TLACGTGACA VVVAALLTGK CDRRCTVELP 250
    GGCLQIHWSQ TDNRVYMTGP AKAVFEGIYP I 281
    Length:281
    Mass (Da):30,590
    Last modified:March 18, 2008 - v1
    Checksum:i46F46003939B0422
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009552 Genomic DNA. Translation: BAG05766.1.
    RefSeqiWP_012268125.1. NC_010296.1.
    YP_001660958.1. NC_010296.1.

    Genome annotation databases

    EnsemblBacteriaiBAG05766; BAG05766; MAE_59440.
    GeneIDi5866788.
    KEGGimar:MAE_59440.
    PATRICi22636297. VBIMicAer59304_5446.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009552 Genomic DNA. Translation: BAG05766.1 .
    RefSeqi WP_012268125.1. NC_010296.1.
    YP_001660958.1. NC_010296.1.

    3D structure databases

    ProteinModelPortali B0JJ58.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 449447.MAE_59440.

    Proteomic databases

    PaxDbi B0JJ58.
    PRIDEi B0JJ58.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG05766 ; BAG05766 ; MAE_59440 .
    GeneIDi 5866788.
    KEGGi mar:MAE_59440.
    PATRICi 22636297. VBIMicAer59304_5446.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220467.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci MAER449447:GHO8-5992-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NIES-843.

    Entry informationi

    Entry nameiDAPF_MICAN
    AccessioniPrimary (citable) accession number: B0JJ58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3