B0JJ58 (DAPF_MICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diaminopimelate epimerase
Short name=DAP epimerase
EC=5.1.1.7

Gene names

Name:	dapF
Ordered Locus Names:	MAE_59440

Organism

Microcystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]

Taxonomic identifier

449447 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Microcystis

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP MF_00197
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP MF_00197
Subcellular location	Cytoplasm By similarity HAMAP MF_00197.
Sequence similarities	Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diaminopimelate epimerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Diaminopimelate epimerase HAMAP MF_00197		PRO_1000077699
Sites
	Active site	75	1	By similarity
	Active site	224	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0JJ58 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 46F46003939B0422	FASTA	281	30,590
	10 20 30 40 50 60 MKIPFTKYQG LGNDFILIDN RHSPEPLITA EMAVAMCDRH FGIGADGVIF ALPGQAETDY 70 80 90 100 110 120 TMRIFNSDGS EPEMCGNGIR CLAQFIARLE ANNAIGRTYR IHTLAGTIIP RLEANEQVTV 130 140 150 160 170 180 DMGPPQLLGS EIPTTLVKGS EKVLAVPLEV EGKDWLVTCV SMGNPHCVTF VGDLASIPLE 190 200 210 220 230 240 TIGPKFEHHP VFPQRTNVEF VEVIAPDYMK MRVWERGAGI TLACGTGACA VVVAALLTGK 250 260 270 280 CDRRCTVELP GGCLQIHWSQ TDNRVYMTGP AKAVFEGIYP I

References

[1]

"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843." Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M. DNA Res. 14:247-256(2007) [PubMed: 18192279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIES-843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009552 Genomic DNA. Translation: BAG05766.1.
RefSeq	YP_001660958.1. NC_010296.1.
3D structure databases
ProteinModelPortal	B0JJ58.
ModBase	Search...
Protein-protein interaction databases
STRING	B0JJ58.
Proteomic databases
PRIDE	B0JJ58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5866788.
GenomeReviews	Gene locus MAE_59440 in contig AP009552_GR.
KEGG	mar:MAE_59440.
PATRIC	22636297. VBIMicAer59304_5446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG399442.
OMA	FMTGPAT.
PhylomeDB	B0JJ58.
ProtClustDB	PRK00450.
Enzyme and pathway databases
BioCyc	MAER449447:MAE_59440-MONOMER.
Family and domain databases
HAMAP	MF_00197. DAP_epimerase. [Tree]
InterPro	IPR018510. DAP_epimerase_AS. IPR001653. DAP_epimerase_DapF. [Graphical view]
KO	K01778.
Pfam	PF01678. DAP_epimerase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00652. DapF. 1 hit.
PROSITE	PS01326. DAP_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPF_MICAN

Accession

Primary (citable) accession number: B0JJ58

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program