B0JGF9 (B0JGF9_MICAN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685 EMBL BAG05225.1
Ordered Locus Names:	MAE_54030

Organism

Microcystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]

Taxonomic identifier

449447 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Microcystis

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	433	1	Nucleophile By similarity HAMAP MF_00685
	Active site	486	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B0JGF9 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: 174026BCC46E743E	FASTA	759	88,125
	10 20 30 40 50 60 MSIVVSPEQV HQIVNNLHHD PFEVLGAHPL EEEGKVNKWV VRAYLPKTTA AWVILPSQRQ 70 80 90 100 110 120 EYPMTTAYHP HFFECVIDIS ELNNYQLRIH EGEQERVIYD SYAFRSAKLS DFDLHLFGEG 130 140 150 160 170 180 NHHRIYEKLG AHLAEIEGIK GVYFAVWAPN ARNVSIIGDF NNWDGRDHQM RKRNNMVWEL 190 200 210 220 230 240 FIPEIGVGTK YKYEIKNWEG HIYEKSDPYG FAQEVRPKTA SIVANLDSYT WDDSDWMEKR 250 260 270 280 290 300 RHSDPLTQPV SVYELHLGSW LHASSAEPPR LLSGEGEAVP VSEWNTGARF LSYYELAQKL 310 320 330 340 350 360 IPYVKELGYT HIELLPIAEH PFDGSWGYQV TGYFAPTSRY GNPEDFMYFV DQCHQNGLGV 370 380 390 400 410 420 LVDWVPGHFP KDGHGLAFFD GTHLYEHGDP RKGEHKEWGT LIFNYGRNEV RNFLVANALF 430 440 450 460 470 480 WFDKYHIDGI RVDAVASMLY LDYCRKDGEW VANEYGGREN LEAAEFLRQV NHVIFSYFPG 490 500 510 520 530 540 ILSIAEESTA WPMVSWPTYM GGLGFNLKWN MGWMHDMLDY FGMDPWFRQF HQNNVTFSMW 550 560 570 580 590 600 YNHSENYMLA LSHDEVVHGK SNMIGKMPGD EWQKFANVRA LFSYMFTHPG KKTMFMSMEF 610 620 630 640 650 660 GQWSEWNVWG DLEWGLLQYE PHQQLKRFFS DLNATYKSEP ALYTQDFGQD GFEWIDCSDN 670 680 690 700 710 720 SHSVVSFLRW SKNWQEFLVV VCNFTPQPHS HYRVGVPHPG FYQEIFNSDA GKYGGSNMGN 730 740 750 LGGKWSEEWS YHSRPYSIDL CLPPLGVLVL KISPNASGE

References

[1]

"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843." Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M. DNA Res. 14:247-256(2007) [PubMed: 18192279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009552 Genomic DNA. Translation: BAG05225.1.
RefSeq	YP_001660417.1. NC_010296.1.
3D structure databases
ProteinModelPortal	B0JGF9.
ModBase	Search...
Protein-protein interaction databases
STRING	B0JGF9.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Proteomic databases
PRIDE	B0JGF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5866242.
GenomeReviews	Gene locus MAE_54030 in contig AP009552_GR.
KEGG	mar:MAE_54030.
PATRIC	22635245. VBIMicAer59304_4925.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	B0JGF9.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B0JGF9_MICAN

Accession

Primary (citable) accession number: B0JGF9

Entry history

Integrated into UniProtKB/TrEMBL:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)