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B0JFP4 (BIOB_MICAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:MAE_00170
OrganismMicrocystis aeruginosa (strain NIES-843) [Complete proteome] [HAMAP]
Taxonomic identifier449447 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 364364Biotin synthase HAMAP-Rule MF_01694
PRO_0000381474

Sites

Metal binding861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding931Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1681Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2281Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2981Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0JFP4 [UniParc].

Last modified March 18, 2008. Version 1.
Checksum: B518DE9300BA8DA9

FASTA36439,354
        10         20         30         40         50         60 
MVQVSTHSAT QSIPMEGEAL KNWLQTRANQ IIAGDRLSKQ EALALTAIEG QENIFSLCEA 

        70         80         90        100        110        120 
ADRIRQACCG NTVDLCSIIN IKSGSCSENC SFCSQSVHHP GQDSPIYGLK SREEIVTHAK 

       130        140        150        160        170        180 
AAADAGAKRF CLVSQGRGLK YNSPKSQEFE EILATVQEII ETAKIKPCCA LGELTLPQAQ 

       190        200        210        220        230        240 
ALKEAGVTRY NHNLEASENF YPNVVSTHSW QDRVETVKNL KAAGIQACTG GIIGMGESWT 

       250        260        270        280        290        300 
DRIDLAFSLA ELEVESVPIN LLNPRSGTPL GHLPKLEPFT ALKAIAIFRF ILPQQILRYA 

       310        320        330        340        350        360 
GGREAVMGEL QNLGLKSGIN AMLIGHYLTT LGQPPQQDQA MLADLSLIGG EAPIPGEYQP 


QQAT 

« Hide

References

[1]"Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843."
Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M., Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M. expand/collapse author list , Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.
DNA Res. 14:247-256(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NIES-843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009552 Genomic DNA. Translation: BAF99838.1.
RefSeqYP_001655031.1. NC_010296.1.

3D structure databases

ProteinModelPortalB0JFP4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING449447.MAE_00170.

Proteomic databases

PaxDbB0JFP4.
PRIDEB0JFP4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF99838; BAF99838; MAE_00170.
GeneID5861134.
KEGGmar:MAE_00170.
PATRIC22625351. VBIMicAer59304_0016.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycMAER449447:GHO8-17-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MICAN
AccessionPrimary (citable) accession number: B0JFP4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 18, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways