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B0JFP4

- BIOB_MICAN

UniProt

B0JFP4 - BIOB_MICAN

Protein

Biotin synthase

Gene

bioB

Organism
Microcystis aeruginosa (strain NIES-843)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi93 – 931Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi168 – 1681Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi228 – 2281Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi298 – 2981Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMAER449447:GHO8-17-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:MAE_00170
    OrganismiMicrocystis aeruginosa (strain NIES-843)
    Taxonomic identifieri449447 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis
    ProteomesiUP000001510: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364Biotin synthasePRO_0000381474Add
    BLAST

    Proteomic databases

    PaxDbiB0JFP4.
    PRIDEiB0JFP4.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi449447.MAE_00170.

    Structurei

    3D structure databases

    ProteinModelPortaliB0JFP4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B0JFP4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVQVSTHSAT QSIPMEGEAL KNWLQTRANQ IIAGDRLSKQ EALALTAIEG    50
    QENIFSLCEA ADRIRQACCG NTVDLCSIIN IKSGSCSENC SFCSQSVHHP 100
    GQDSPIYGLK SREEIVTHAK AAADAGAKRF CLVSQGRGLK YNSPKSQEFE 150
    EILATVQEII ETAKIKPCCA LGELTLPQAQ ALKEAGVTRY NHNLEASENF 200
    YPNVVSTHSW QDRVETVKNL KAAGIQACTG GIIGMGESWT DRIDLAFSLA 250
    ELEVESVPIN LLNPRSGTPL GHLPKLEPFT ALKAIAIFRF ILPQQILRYA 300
    GGREAVMGEL QNLGLKSGIN AMLIGHYLTT LGQPPQQDQA MLADLSLIGG 350
    EAPIPGEYQP QQAT 364
    Length:364
    Mass (Da):39,354
    Last modified:March 18, 2008 - v1
    Checksum:iB518DE9300BA8DA9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009552 Genomic DNA. Translation: BAF99838.1.
    RefSeqiYP_001655031.1. NC_010296.1.

    Genome annotation databases

    EnsemblBacteriaiBAF99838; BAF99838; MAE_00170.
    GeneIDi5861134.
    KEGGimar:MAE_00170.
    PATRICi22625351. VBIMicAer59304_0016.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009552 Genomic DNA. Translation: BAF99838.1 .
    RefSeqi YP_001655031.1. NC_010296.1.

    3D structure databases

    ProteinModelPortali B0JFP4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 449447.MAE_00170.

    Proteomic databases

    PaxDbi B0JFP4.
    PRIDEi B0JFP4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF99838 ; BAF99838 ; MAE_00170 .
    GeneIDi 5861134.
    KEGGi mar:MAE_00170.
    PATRICi 22625351. VBIMicAer59304_0016.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci MAER449447:GHO8-17-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NIES-843.

    Entry informationi

    Entry nameiBIOB_MICAN
    AccessioniPrimary (citable) accession number: B0JFP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3