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B0JFP4

- BIOB_MICAN

UniProt

B0JFP4 - BIOB_MICAN

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Protein

Biotin synthase

Gene
bioB, MAE_00170
Organism
Microcystis aeruginosa (strain NIES-843)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi93 – 931Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi168 – 1681Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi228 – 2281Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi298 – 2981Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMAER449447:GHO8-17-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MAE_00170
OrganismiMicrocystis aeruginosa (strain NIES-843)
Taxonomic identifieri449447 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesMicrocystis
ProteomesiUP000001510: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Biotin synthaseUniRule annotationPRO_0000381474Add
BLAST

Proteomic databases

PaxDbiB0JFP4.
PRIDEiB0JFP4.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi449447.MAE_00170.

Structurei

3D structure databases

ProteinModelPortaliB0JFP4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B0JFP4-1 [UniParc]FASTAAdd to Basket

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MVQVSTHSAT QSIPMEGEAL KNWLQTRANQ IIAGDRLSKQ EALALTAIEG    50
QENIFSLCEA ADRIRQACCG NTVDLCSIIN IKSGSCSENC SFCSQSVHHP 100
GQDSPIYGLK SREEIVTHAK AAADAGAKRF CLVSQGRGLK YNSPKSQEFE 150
EILATVQEII ETAKIKPCCA LGELTLPQAQ ALKEAGVTRY NHNLEASENF 200
YPNVVSTHSW QDRVETVKNL KAAGIQACTG GIIGMGESWT DRIDLAFSLA 250
ELEVESVPIN LLNPRSGTPL GHLPKLEPFT ALKAIAIFRF ILPQQILRYA 300
GGREAVMGEL QNLGLKSGIN AMLIGHYLTT LGQPPQQDQA MLADLSLIGG 350
EAPIPGEYQP QQAT 364
Length:364
Mass (Da):39,354
Last modified:March 18, 2008 - v1
Checksum:iB518DE9300BA8DA9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009552 Genomic DNA. Translation: BAF99838.1.
RefSeqiYP_001655031.1. NC_010296.1.

Genome annotation databases

EnsemblBacteriaiBAF99838; BAF99838; MAE_00170.
GeneIDi5861134.
KEGGimar:MAE_00170.
PATRICi22625351. VBIMicAer59304_0016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009552 Genomic DNA. Translation: BAF99838.1 .
RefSeqi YP_001655031.1. NC_010296.1.

3D structure databases

ProteinModelPortali B0JFP4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 449447.MAE_00170.

Proteomic databases

PaxDbi B0JFP4.
PRIDEi B0JFP4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF99838 ; BAF99838 ; MAE_00170 .
GeneIDi 5861134.
KEGGi mar:MAE_00170.
PATRICi 22625351. VBIMicAer59304_0016.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MAER449447:GHO8-17-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NIES-843.

Entry informationi

Entry nameiBIOB_MICAN
AccessioniPrimary (citable) accession number: B0JFP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 18, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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