B0I1T2 (MYO1G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Unconventional myosin-Ig

Cleaved into the following chain:

1. Minor histocompatibility antigen HA-2
   Short name=mHag HA-2

Gene names

Name:	MYO1G
Synonyms:	HA2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1018 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments By similarity. Precursor of the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.
Subunit structure	Binds calmodulin through its IQ motifs By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein. Note: Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles. Ref.7
Tissue specificity	Specifically expressed in hematopoietic cells. Ref.4 Ref.7
Sequence similarities	Contains 1 IQ domain. Contains 1 myosin head-like domain.
Caution	Represents a unconventional myosin. This protein should not be confused with the conventional myosin-1 (MYH1).
Sequence caution	The sequence BAB84876.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Actin-binding Calmodulin-binding Nucleotide-binding
Molecular function	Motor protein Myosin
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	myosin complex Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 19968988. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B0I1T2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B0I1T2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     207-230: LLRGSEDKQLHELHLERNPAVYNF → VSPEGKGRWKNGVGKGRAASWTSL
     231-1018: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: B0I1T2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     526-1018: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: B0I1T2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     652-722: KMTCEYTWPN...IVLLLQKAWR → WHLTPITPWA...TGGNMGMRAV
     723-1018: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1018	1018	Unconventional myosin-Ig		PRO_0000340316
	Peptide	41 – 49	9	Minor histocompatibility antigen HA-2 Ref.5		PRO_0000340317
Regions
	Domain	13 – 683	671	Myosin head-like
	Domain	710 – 739	30	IQ
	Nucleotide binding	102 – 109	8	ATP By similarity
Natural variations
	Alternative sequence	207 – 230	24	LLRGS…AVYNF → VSPEGKGRWKNGVGKGRAAS WTSL in isoform 2.		VSP_034208
	Alternative sequence	231 – 1018	788	Missing in isoform 2.		VSP_034209
	Alternative sequence	526 – 1018	493	Missing in isoform 3.		VSP_034210
	Alternative sequence	652 – 722	71	KMTCE…QKAWR → WHLTPITPWAIVPVWSPRGR SRGSPNSTSQTSIQAGTSTL LASRHQNIWEDMCVSTCMWG HTGGNMGMRAV in isoform 4.		VSP_034211
	Alternative sequence	723 – 1018	296	Missing in isoform 4.		VSP_034212
	Natural variant	49	1	V → M in allele HA-2M; the HA-2V allele constitute the HA-2 epitope while HA-2M is not recognized by HA-2 cytotoxic T lymphocytes. Ref.4		VAR_044013
	Natural variant	489	1	M → T. Ref.1 Ref.2 Ref.4 Corresponds to variant rs3735485 [ dbSNP | Ensembl ].		VAR_044014
	Natural variant	798	1	R → Q. Corresponds to variant rs2107737 [ dbSNP | Ensembl ].		VAR_050212
	Natural variant	861	1	Q → R. Ref.2 Ref.6 Corresponds to variant rs7792760 [ dbSNP | Ensembl ].		VAR_044015
Experimental info
	Mutagenesis	815	1	K → A: Reduced membrane association. Ref.7
	Mutagenesis	826	1	R → A: Reduced membrane association. Ref.7
	Mutagenesis	876	1	R → A: No effect on membrane localization. Ref.7
	Mutagenesis	880	1	R → A: No effect on membrane localization. Ref.7
	Mutagenesis	883	1	K → A: No effect on membrane localization; when associated with R-885.
	Mutagenesis	885	1	R → A: No effect on membrane localization; when associated with K-883. Ref.7
	Mutagenesis	898	1	K → A: Reduced membrane association. Ref.7
	Mutagenesis	903	1	R → A: No effect on membrane localization; when associated with R-906. Ref.7
	Mutagenesis	906	1	R → A: No effect on membrane localization; when associated with R-903. Ref.7
	Mutagenesis	909	1	R → A: No effect on membrane localization. Ref.7
	Mutagenesis	934	1	R → A: No effect on membrane localization. Ref.7
	Mutagenesis	945	1	R → A: No effect on membrane localization. Ref.7
	Mutagenesis	947	1	R → A: No effect on membrane localization. Ref.7
	Mutagenesis	953	1	R → A: No effect on membrane localization. Ref.7
	Sequence conflict	369	1	N → K in BAB84961. Ref.1
	Sequence conflict	377	1	P → L in AAK58092. Ref.4
	Sequence conflict	377	1	P → L in AAK58093. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 2. Checksum: 3EB4ACC3D99A86E9	FASTA	1,018	116,442
	10 20 30 40 50 60 MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN PYQELPLYGP 70 80 90 100 110 120 EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI SGESGAGKTE ASKHIMQYIA 130 140 150 160 170 180 AVTNPSQRAE VERVKDVLLK STCVLEAFGN ARTNRNHNSS RFGKYMDINF DFKGDPIGGH 190 200 210 220 230 240 IHSYLLEKSR VLKQHVGERN FHAFYQLLRG SEDKQLHELH LERNPAVYNF THQGAGLNMT 250 260 270 280 290 300 VHSALDSDEQ SHQAVTEAMR VIGFSPEEVE SVHRILAAIL HLGNIEFVET EEGGLQKEGL 310 320 330 340 350 360 AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY ARDACAKAVY 370 380 390 400 410 420 QRLFEWVVNR INSVMEPRGR DPRRDGKDTV IGVLDIYGFE VFPVNSFEQF CINYCNEKLQ 430 440 450 460 470 480 QLFIQLILKQ EQEEYEREGI TWQSVEYFNN ATIVDLVERP HRGILAVLDE ACSSAGTITD 490 500 510 520 530 540 RIFLQTLDMH HRHHLHYTSR QLCPTDKTME FGRDFRIKHY AGDVTYSVEG FIDKNRDFLF 550 560 570 580 590 600 QDFKRLLYNS TDPTLRAMWP DGQQDITEVT KRPLTAGTLF KNSMVALVEN LASKEPFYVR 610 620 630 640 650 660 CIKPNEDKVA GKLDENHCRH QVAYLGLLEN VRVRRAGFAS RQPYSRFLLR YKMTCEYTWP 670 680 690 700 710 720 NHLLGSDKAA VSALLEQHGL QGDVAFGHSK LFIRSPRTLV TLEQSRARLI PIIVLLLQKA 730 740 750 760 770 780 WRGTLARWRC RRLRAIYTIM RWFRRHKVRA HLAELQRRFQ AARQPPLYGR DLVWPLPPAV 790 800 810 820 830 840 LQPFQDTCHA LFCRWRARQL VKNIPPSDMP QIKAKVAAMG ALQGLRQDWG CRRAWARDYL 850 860 870 880 890 900 SSATDNPTAS SLFAQRLKTL QDKDGFGAVL FSSHVRKVNR FHKIRNRALL LTDQHLYKLD 910 920 930 940 950 960 PDRQYRVMRA VPLEAVTGLS VTSGGDQLVV LHARGQDDLV VCLHRSRPPL DNRVGELVGV 970 980 990 1000 1010 LAAHCQGEGR TLEVRVSDCI PLSHRGVRRL ISVEPRPEQP EPDFRCARGS FTLLWPSR
	Isoform 2 [UniParc]. Checksum: 7A80030FE7FCCE28	FASTA	230	26,151
	10 20 30 40 50 60 MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN PYQELPLYGP 70 80 90 100 110 120 EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI SGESGAGKTE ASKHIMQYIA 130 140 150 160 170 180 AVTNPSQRAE VERVKDVLLK STCVLEAFGN ARTNRNHNSS RFGKYMDINF DFKGDPIGGH 190 200 210 220 230 IHSYLLEKSR VLKQHVGERN FHAFYQVSPE GKGRWKNGVG KGRAASWTSL
	Isoform 3 [UniParc]. Checksum: 1B25EB82195E4993	FASTA	525	59,815
	10 20 30 40 50 60 MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN PYQELPLYGP 70 80 90 100 110 120 EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI SGESGAGKTE ASKHIMQYIA 130 140 150 160 170 180 AVTNPSQRAE VERVKDVLLK STCVLEAFGN ARTNRNHNSS RFGKYMDINF DFKGDPIGGH 190 200 210 220 230 240 IHSYLLEKSR VLKQHVGERN FHAFYQLLRG SEDKQLHELH LERNPAVYNF THQGAGLNMT 250 260 270 280 290 300 VHSALDSDEQ SHQAVTEAMR VIGFSPEEVE SVHRILAAIL HLGNIEFVET EEGGLQKEGL 310 320 330 340 350 360 AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY ARDACAKAVY 370 380 390 400 410 420 QRLFEWVVNR INSVMEPRGR DPRRDGKDTV IGVLDIYGFE VFPVNSFEQF CINYCNEKLQ 430 440 450 460 470 480 QLFIQLILKQ EQEEYEREGI TWQSVEYFNN ATIVDLVERP HRGILAVLDE ACSSAGTITD 490 500 510 520 RIFLQTLDMH HRHHLHYTSR QLCPTDKTME FGRDFRIKHY AGDVT
	Isoform 4 [UniParc]. Checksum: 6275117D9D1751C1	FASTA	722	82,279
	10 20 30 40 50 60 MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN PYQELPLYGP 70 80 90 100 110 120 EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI SGESGAGKTE ASKHIMQYIA 130 140 150 160 170 180 AVTNPSQRAE VERVKDVLLK STCVLEAFGN ARTNRNHNSS RFGKYMDINF DFKGDPIGGH 190 200 210 220 230 240 IHSYLLEKSR VLKQHVGERN FHAFYQLLRG SEDKQLHELH LERNPAVYNF THQGAGLNMT 250 260 270 280 290 300 VHSALDSDEQ SHQAVTEAMR VIGFSPEEVE SVHRILAAIL HLGNIEFVET EEGGLQKEGL 310 320 330 340 350 360 AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY ARDACAKAVY 370 380 390 400 410 420 QRLFEWVVNR INSVMEPRGR DPRRDGKDTV IGVLDIYGFE VFPVNSFEQF CINYCNEKLQ 430 440 450 460 470 480 QLFIQLILKQ EQEEYEREGI TWQSVEYFNN ATIVDLVERP HRGILAVLDE ACSSAGTITD 490 500 510 520 530 540 RIFLQTLDMH HRHHLHYTSR QLCPTDKTME FGRDFRIKHY AGDVTYSVEG FIDKNRDFLF 550 560 570 580 590 600 QDFKRLLYNS TDPTLRAMWP DGQQDITEVT KRPLTAGTLF KNSMVALVEN LASKEPFYVR 610 620 630 640 650 660 CIKPNEDKVA GKLDENHCRH QVAYLGLLEN VRVRRAGFAS RQPYSRFLLR YWHLTPITPW 670 680 690 700 710 720 AIVPVWSPRG RSRGSPNSTS QTSIQAGTST LLASRHQNIW EDMCVSTCMW GHTGGNMGMR AV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 193-1018 (ISOFORM 3), VARIANT THR-489. Tissue: Spleen.
[2]	"Multiplex amplification and cloning of 5'-ends of cDNA by ligase-free recombination: preparation of full-length cDNA clones encoding motor proteins." Yamakawa H., Kikuno R.F., Nagase T., Ohara O. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS THR-489 AND ARG-861. Tissue: Spleen.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The HA-2 minor histocompatibility antigen is derived from a diallelic gene encoding a novel human class I myosin protein." Pierce R.A., Field E.D., Mutis T., Golovina T.N., Von Kap-Herr C., Wilke M., Pool J., Shabanowitz J., Pettenati M.J., Eisenlohr L.C., Hunt D.F., Goulmy E., Engelhard V.H. J. Immunol. 167:3223-3230(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-665 (ISOFORM 4), IDENTIFICATION AS THE MINOR HISTOCOMPATIBILITY ANTIGEN HA-2, VARIANTS HA-2M MET-49 AND THR-489, CHARACTERIZATION OF VARIANT HA-2M MET-49, TISSUE SPECIFICITY.
[5]	"Identification of a graft versus host disease-associated human minor histocompatibility antigen." den Haan J.M.M., Sherman N.E., Blokland E., Huczko E., Koning F., Drijfhout J.W., Skipper J., Shabanowitz J., Hunt D.F., Engelhard V.H., Goulmy E. Science 268:1476-1480(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 41-49 (ISOFORM 1).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 723-1018 (ISOFORM 1), VARIANT ARG-861. Tissue: B-cell.
[7]	"Myosin 1G is an abundant class I myosin in lymphocytes whose localization at the plasma membrane depends on its ancient divergent pleckstrin homology (PH) domain (Myo1PH)." Patino-Lopez G., Aravind L., Dong X., Kruhlak M.J., Ostap E.M., Shaw S. J. Biol. Chem. 285:8675-8686(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-815; ARG-826; ARG-876; ARG-880; ARG-885; LYS-898; ARG-903; ARG-906; ARG-909; ARG-934; ARG-945; ARG-947 AND ARG-953.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK074050 mRNA. Translation: BAB84876.1. Sequence problems. AK074135 mRNA. Translation: BAB84961.1. AB290179 mRNA. Translation: BAG06733.1. AC004847 Genomic DNA. No translation available. AF380932 mRNA. Translation: AAK58092.1. AF380933 mRNA. Translation: AAK58093.1. BC015693 mRNA. Translation: AAH15693.2.
IPI	IPI00420004. IPI00895790. IPI00895883. IPI00895957.
RefSeq	NP_149043.2. NM_033054.2.
UniGene	Hs.37617.
3D structure databases
ProteinModelPortal	B0I1T2.
SMR	B0I1T2. Positions 10-711.
ModBase	Search...
Protein-protein interaction databases
IntAct	B0I1T2. 2 interactions.
MINT	MINT-7307233.
STRING	9606.ENSP00000258787.
PTM databases
PhosphoSite	B0I1T2.
Proteomic databases
PaxDb	B0I1T2.
PRIDE	B0I1T2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000258787; ENSP00000258787; ENSG00000136286.
GeneID	64005.
KEGG	hsa:64005.
UCSC	uc003tmg.2. human. uc003tmi.1. human. uc003tmj.2. human.
Organism-specific databases
CTD	64005.
GeneCards	GC07M045003.
H-InvDB	HIX0006659.
HGNC	HGNC:13880. MYO1G.
HPA	HPA021252.
MIM	613445. gene.
neXtProt	NX_B0I1T2.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5022.
HOGENOM	HOG000260264.
HOVERGEN	HBG062373.
InParanoid	B0I1T2.
KO	K10356.
OMA	EQHGLQG.
OrthoDB	EOG4Z36CZ.
Gene expression databases
ArrayExpress	B0I1T2.
Bgee	B0I1T2.
CleanEx	HS_MYO1G.
Genevestigator	B0I1T2.
Family and domain databases
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR001609. Myosin_head_motor_dom. IPR010926. Myosin_tail_2. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00063. Myosin_head. 1 hit. PF06017. Myosin_TH1. 1 hit. [Graphical view]
PRINTS	PR00193. MYOSINHEAVY.
SMART	SM00015. IQ. 1 hit. SM00242. MYSc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS50096. IQ. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	64005.
NextBio	65808.
SOURCE	Search...

Entry information

Entry name

MYO1G_HUMAN

Accession

Primary (citable) accession number: B0I1T2
Secondary accession number(s): Q8TEI9 , Q8TES2, Q96BE2, Q96RI5, Q96RI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	January 11, 2011
Last modified:	May 29, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.