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Protein

Unconventional myosin-Ig

Gene

MYO1G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Unconventional myosin required during immune response for detection of rare antigen-presenting cells by regulating T-cell migration. Unconventional myosins are actin-based motor molecules with ATPase activity and serve in intracellular movements. Acts as a regulator of T-cell migration by generating membrane tension, enforcing cell-intrinsic meandering search, thereby enhancing detection of rare antigens during lymph-node surveillance, enabling pathogen eradication. Also required in B-cells, where it regulates different membrane/cytoskeleton-dependent processes. Involved in Fc-gamma receptor (Fc-gamma-R) phagocytosis.By similarity
Minor histocompatibility antigen HA-2: Constitutes the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.Curated1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi102 – 109ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Adaptive immunity, Immunity

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Lipid-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-Ig
Cleaved into the following chain:
Minor histocompatibility antigen HA-21 Publication
Short name:
mHag HA-2
Gene namesi
Name:MYO1G
Synonyms:HA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:13880. MYO1G.

Subcellular locationi

  • Cell membrane 2 Publications; Peripheral membrane protein 2 Publications
  • Cell projectionphagocytic cup By similarity

  • Note: Recruited to Fc-gamma receptor (Fc-gamma-R) phagocytic cup. In T-cells, transiently accumulates in discrete areas at the plasma membrane of migrating cells or when membranes are deformed (By similarity). Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles.By similarity1 Publication

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • filopodium Source: Ensembl
  • lamellipodium Source: Ensembl
  • leading edge membrane Source: Ensembl
  • membrane Source: UniProtKB
  • microvillus Source: Ensembl
  • myosin complex Source: UniProtKB-KW
  • phagocytic cup Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi815K → A: Reduced membrane association. 1 Publication1
Mutagenesisi826R → A: Reduced membrane association. 1 Publication1
Mutagenesisi876R → A: No effect on membrane localization. 1 Publication1
Mutagenesisi880R → A: No effect on membrane localization. 1 Publication1
Mutagenesisi883K → A: No effect on membrane localization; when associated with R-885. 1
Mutagenesisi885R → A: No effect on membrane localization; when associated with K-883. 1 Publication1
Mutagenesisi898K → A: Reduced membrane association. 1 Publication1
Mutagenesisi903R → A: No effect on membrane localization; when associated with R-906. 1 Publication1
Mutagenesisi906R → A: No effect on membrane localization; when associated with R-903. 1 Publication1
Mutagenesisi909R → A: No effect on membrane localization. 1 Publication1
Mutagenesisi934R → A: No effect on membrane localization. 1 Publication1
Mutagenesisi945R → A: No effect on membrane localization. 1 Publication1
Mutagenesisi947R → A: No effect on membrane localization. 1 Publication1
Mutagenesisi953R → A: No effect on membrane localization. 1 Publication1

Organism-specific databases

DisGeNETi64005.
OpenTargetsiENSG00000136286.

Polymorphism and mutation databases

BioMutaiMYO1G.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003403161 – 1018Unconventional myosin-IgAdd BLAST1018
PeptideiPRO_000034031741 – 49Minor histocompatibility antigen HA-21 Publication9

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiB0I1T2.
MaxQBiB0I1T2.
PaxDbiB0I1T2.
PRIDEiB0I1T2.

PTM databases

iPTMnetiB0I1T2.
PhosphoSitePlusiB0I1T2.
SwissPalmiB0I1T2.

Expressioni

Tissue specificityi

Specifically expressed in hematopoietic cells.2 Publications

Gene expression databases

BgeeiENSG00000136286.
CleanExiHS_MYO1G.
ExpressionAtlasiB0I1T2. baseline and differential.
GenevisibleiB0I1T2. HS.

Organism-specific databases

HPAiHPA021252.

Interactioni

Subunit structurei

Interacts with calmodulin; via its IQ motifs.By similarity

Protein-protein interaction databases

BioGridi122031. 7 interactors.
IntActiB0I1T2. 8 interactors.
MINTiMINT-7307233.
STRINGi9606.ENSP00000258787.

Structurei

3D structure databases

ProteinModelPortaliB0I1T2.
SMRiB0I1T2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 707Myosin motorAdd BLAST699
Domaini710 – 739IQAdd BLAST30
Domaini824 – 1017TH1PROSITE-ProRule annotationAdd BLAST194

Domaini

The myosin tail domain mediates binding to phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P2), phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) and binds to membranous compartments. It is required for recruitment to Fc-gamma receptor (Fc-gamma-R) phagocytic cups.By similarity

Sequence similaritiesi

Contains 1 IQ domain.Curated
Contains 1 myosin motor domain.Curated
Contains 1 TH1 (class I myosin tail homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
KOG0164. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000260264.
HOVERGENiHBG062373.
InParanoidiB0I1T2.
KOiK10356.
OMAiTLARWRC.
OrthoDBiEOG091G0136.
PhylomeDBiB0I1T2.
TreeFamiTF312960.

Family and domain databases

InterProiIPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B0I1T2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN
60 70 80 90 100
PYQELPLYGP EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI
110 120 130 140 150
SGESGAGKTE ASKHIMQYIA AVTNPSQRAE VERVKDVLLK STCVLEAFGN
160 170 180 190 200
ARTNRNHNSS RFGKYMDINF DFKGDPIGGH IHSYLLEKSR VLKQHVGERN
210 220 230 240 250
FHAFYQLLRG SEDKQLHELH LERNPAVYNF THQGAGLNMT VHSALDSDEQ
260 270 280 290 300
SHQAVTEAMR VIGFSPEEVE SVHRILAAIL HLGNIEFVET EEGGLQKEGL
310 320 330 340 350
AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY
360 370 380 390 400
ARDACAKAVY QRLFEWVVNR INSVMEPRGR DPRRDGKDTV IGVLDIYGFE
410 420 430 440 450
VFPVNSFEQF CINYCNEKLQ QLFIQLILKQ EQEEYEREGI TWQSVEYFNN
460 470 480 490 500
ATIVDLVERP HRGILAVLDE ACSSAGTITD RIFLQTLDMH HRHHLHYTSR
510 520 530 540 550
QLCPTDKTME FGRDFRIKHY AGDVTYSVEG FIDKNRDFLF QDFKRLLYNS
560 570 580 590 600
TDPTLRAMWP DGQQDITEVT KRPLTAGTLF KNSMVALVEN LASKEPFYVR
610 620 630 640 650
CIKPNEDKVA GKLDENHCRH QVAYLGLLEN VRVRRAGFAS RQPYSRFLLR
660 670 680 690 700
YKMTCEYTWP NHLLGSDKAA VSALLEQHGL QGDVAFGHSK LFIRSPRTLV
710 720 730 740 750
TLEQSRARLI PIIVLLLQKA WRGTLARWRC RRLRAIYTIM RWFRRHKVRA
760 770 780 790 800
HLAELQRRFQ AARQPPLYGR DLVWPLPPAV LQPFQDTCHA LFCRWRARQL
810 820 830 840 850
VKNIPPSDMP QIKAKVAAMG ALQGLRQDWG CRRAWARDYL SSATDNPTAS
860 870 880 890 900
SLFAQRLKTL QDKDGFGAVL FSSHVRKVNR FHKIRNRALL LTDQHLYKLD
910 920 930 940 950
PDRQYRVMRA VPLEAVTGLS VTSGGDQLVV LHARGQDDLV VCLHRSRPPL
960 970 980 990 1000
DNRVGELVGV LAAHCQGEGR TLEVRVSDCI PLSHRGVRRL ISVEPRPEQP
1010
EPDFRCARGS FTLLWPSR
Length:1,018
Mass (Da):116,442
Last modified:January 11, 2011 - v2
Checksum:i3EB4ACC3D99A86E9
GO
Isoform 2 (identifier: B0I1T2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     207-230: LLRGSEDKQLHELHLERNPAVYNF → VSPEGKGRWKNGVGKGRAASWTSL
     231-1018: Missing.

Note: No experimental confirmation available.
Show »
Length:230
Mass (Da):26,151
Checksum:i7A80030FE7FCCE28
GO
Isoform 3 (identifier: B0I1T2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     526-1018: Missing.

Note: No experimental confirmation available.
Show »
Length:525
Mass (Da):59,815
Checksum:i1B25EB82195E4993
GO
Isoform 4 (identifier: B0I1T2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     652-722: KMTCEYTWPN...IVLLLQKAWR → WHLTPITPWA...TGGNMGMRAV
     723-1018: Missing.

Note: No experimental confirmation available.
Show »
Length:722
Mass (Da):82,279
Checksum:i6275117D9D1751C1
GO

Sequence cautioni

The sequence BAB84876 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti369N → K in BAB84961 (Ref. 1) Curated1
Sequence conflicti377P → L in AAK58092 (PubMed:11544309).Curated1
Sequence conflicti377P → L in AAK58093 (PubMed:11544309).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04401349V → M in allele HA-2M; the HA-2V allele constitute the HA-2 epitope while HA-2M is not recognized by HA-2 cytotoxic T lymphocytes. 1 PublicationCorresponds to variant rs61739531dbSNPEnsembl.1
Natural variantiVAR_044014489M → T.3 PublicationsCorresponds to variant rs3735485dbSNPEnsembl.1
Natural variantiVAR_050212798R → Q.Corresponds to variant rs2107737dbSNPEnsembl.1
Natural variantiVAR_044015861Q → R.2 PublicationsCorresponds to variant rs7792760dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034208207 – 230LLRGS…AVYNF → VSPEGKGRWKNGVGKGRAAS WTSL in isoform 2. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_034209231 – 1018Missing in isoform 2. 1 PublicationAdd BLAST788
Alternative sequenceiVSP_034210526 – 1018Missing in isoform 3. 1 PublicationAdd BLAST493
Alternative sequenceiVSP_034211652 – 722KMTCE…QKAWR → WHLTPITPWAIVPVWSPRGR SRGSPNSTSQTSIQAGTSTL LASRHQNIWEDMCVSTCMWG HTGGNMGMRAV in isoform 4. 1 PublicationAdd BLAST71
Alternative sequenceiVSP_034212723 – 1018Missing in isoform 4. 1 PublicationAdd BLAST296

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074050 mRNA. Translation: BAB84876.1. Sequence problems.
AK074135 mRNA. Translation: BAB84961.1.
AB290179 mRNA. Translation: BAG06733.1.
AC004847 Genomic DNA. No translation available.
AF380932 mRNA. Translation: AAK58092.1.
AF380933 mRNA. Translation: AAK58093.1.
BC015693 mRNA. Translation: AAH15693.2.
CCDSiCCDS34629.1. [B0I1T2-1]
RefSeqiNP_149043.2. NM_033054.2. [B0I1T2-1]
UniGeneiHs.37617.

Genome annotation databases

EnsembliENST00000258787; ENSP00000258787; ENSG00000136286. [B0I1T2-1]
GeneIDi64005.
KEGGihsa:64005.
UCSCiuc003tmh.3. human. [B0I1T2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK074050 mRNA. Translation: BAB84876.1. Sequence problems.
AK074135 mRNA. Translation: BAB84961.1.
AB290179 mRNA. Translation: BAG06733.1.
AC004847 Genomic DNA. No translation available.
AF380932 mRNA. Translation: AAK58092.1.
AF380933 mRNA. Translation: AAK58093.1.
BC015693 mRNA. Translation: AAH15693.2.
CCDSiCCDS34629.1. [B0I1T2-1]
RefSeqiNP_149043.2. NM_033054.2. [B0I1T2-1]
UniGeneiHs.37617.

3D structure databases

ProteinModelPortaliB0I1T2.
SMRiB0I1T2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122031. 7 interactors.
IntActiB0I1T2. 8 interactors.
MINTiMINT-7307233.
STRINGi9606.ENSP00000258787.

PTM databases

iPTMnetiB0I1T2.
PhosphoSitePlusiB0I1T2.
SwissPalmiB0I1T2.

Polymorphism and mutation databases

BioMutaiMYO1G.

Proteomic databases

EPDiB0I1T2.
MaxQBiB0I1T2.
PaxDbiB0I1T2.
PRIDEiB0I1T2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000258787; ENSP00000258787; ENSG00000136286. [B0I1T2-1]
GeneIDi64005.
KEGGihsa:64005.
UCSCiuc003tmh.3. human. [B0I1T2-1]

Organism-specific databases

CTDi64005.
DisGeNETi64005.
GeneCardsiMYO1G.
H-InvDBHIX0006659.
HGNCiHGNC:13880. MYO1G.
HPAiHPA021252.
MIMi600642. gene.
neXtProtiNX_B0I1T2.
OpenTargetsiENSG00000136286.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
KOG0164. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000260264.
HOVERGENiHBG062373.
InParanoidiB0I1T2.
KOiK10356.
OMAiTLARWRC.
OrthoDBiEOG091G0136.
PhylomeDBiB0I1T2.
TreeFamiTF312960.

Miscellaneous databases

GenomeRNAii64005.
PROiB0I1T2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136286.
CleanExiHS_MYO1G.
ExpressionAtlasiB0I1T2. baseline and differential.
GenevisibleiB0I1T2. HS.

Family and domain databases

InterProiIPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO1G_HUMAN
AccessioniPrimary (citable) accession number: B0I1T2
Secondary accession number(s): Q8TEI9
, Q8TES2, Q96BE2, Q96RI5, Q96RI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-1 (MYH1).Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.