Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B0I1T2 (MYO1G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Unconventional myosin-Ig

Cleaved into the following chain:

  1. Minor histocompatibility antigen HA-2
    Short name=mHag HA-2
Gene names
Name:MYO1G
Synonyms:HA2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1018 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments By similarity.

Precursor of the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.

Subunit structure

Binds calmodulin through its IQ motifs By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein. Note: Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles. Ref.7

Tissue specificity

Specifically expressed in hematopoietic cells. Ref.4 Ref.7

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Contains 1 IQ domain.

Contains 1 myosin motor domain.

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-1 (MYH1).

Sequence caution

The sequence BAB84876.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: B0I1T2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B0I1T2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     207-230: LLRGSEDKQLHELHLERNPAVYNF → VSPEGKGRWKNGVGKGRAASWTSL
     231-1018: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: B0I1T2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     526-1018: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: B0I1T2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     652-722: KMTCEYTWPN...IVLLLQKAWR → WHLTPITPWA...TGGNMGMRAV
     723-1018: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10181018Unconventional myosin-Ig
PRO_0000340316
Peptide41 – 499Minor histocompatibility antigen HA-2 Ref.5
PRO_0000340317

Regions

Domain9 – 707699Myosin motor
Domain710 – 73930IQ
Nucleotide binding102 – 1098ATP By similarity

Natural variations

Alternative sequence207 – 23024LLRGS…AVYNF → VSPEGKGRWKNGVGKGRAAS WTSL in isoform 2.
VSP_034208
Alternative sequence231 – 1018788Missing in isoform 2.
VSP_034209
Alternative sequence526 – 1018493Missing in isoform 3.
VSP_034210
Alternative sequence652 – 72271KMTCE…QKAWR → WHLTPITPWAIVPVWSPRGR SRGSPNSTSQTSIQAGTSTL LASRHQNIWEDMCVSTCMWG HTGGNMGMRAV in isoform 4.
VSP_034211
Alternative sequence723 – 1018296Missing in isoform 4.
VSP_034212
Natural variant491V → M in allele HA-2M; the HA-2V allele constitute the HA-2 epitope while HA-2M is not recognized by HA-2 cytotoxic T lymphocytes. Ref.4
Corresponds to variant rs61739531 [ dbSNP | Ensembl ].
VAR_044013
Natural variant4891M → T. Ref.1 Ref.2 Ref.4
Corresponds to variant rs3735485 [ dbSNP | Ensembl ].
VAR_044014
Natural variant7981R → Q.
Corresponds to variant rs2107737 [ dbSNP | Ensembl ].
VAR_050212
Natural variant8611Q → R. Ref.2 Ref.6
Corresponds to variant rs7792760 [ dbSNP | Ensembl ].
VAR_044015

Experimental info

Mutagenesis8151K → A: Reduced membrane association. Ref.7
Mutagenesis8261R → A: Reduced membrane association. Ref.7
Mutagenesis8761R → A: No effect on membrane localization. Ref.7
Mutagenesis8801R → A: No effect on membrane localization. Ref.7
Mutagenesis8831K → A: No effect on membrane localization; when associated with R-885.
Mutagenesis8851R → A: No effect on membrane localization; when associated with K-883. Ref.7
Mutagenesis8981K → A: Reduced membrane association. Ref.7
Mutagenesis9031R → A: No effect on membrane localization; when associated with R-906. Ref.7
Mutagenesis9061R → A: No effect on membrane localization; when associated with R-903. Ref.7
Mutagenesis9091R → A: No effect on membrane localization. Ref.7
Mutagenesis9341R → A: No effect on membrane localization. Ref.7
Mutagenesis9451R → A: No effect on membrane localization. Ref.7
Mutagenesis9471R → A: No effect on membrane localization. Ref.7
Mutagenesis9531R → A: No effect on membrane localization. Ref.7
Sequence conflict3691N → K in BAB84961. Ref.1
Sequence conflict3771P → L in AAK58092. Ref.4
Sequence conflict3771P → L in AAK58093. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2011. Version 2.
Checksum: 3EB4ACC3D99A86E9

FASTA1,018116,442
        10         20         30         40         50         60 
MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN PYQELPLYGP 

        70         80         90        100        110        120 
EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI SGESGAGKTE ASKHIMQYIA 

       130        140        150        160        170        180 
AVTNPSQRAE VERVKDVLLK STCVLEAFGN ARTNRNHNSS RFGKYMDINF DFKGDPIGGH 

       190        200        210        220        230        240 
IHSYLLEKSR VLKQHVGERN FHAFYQLLRG SEDKQLHELH LERNPAVYNF THQGAGLNMT 

       250        260        270        280        290        300 
VHSALDSDEQ SHQAVTEAMR VIGFSPEEVE SVHRILAAIL HLGNIEFVET EEGGLQKEGL 

       310        320        330        340        350        360 
AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY ARDACAKAVY 

       370        380        390        400        410        420 
QRLFEWVVNR INSVMEPRGR DPRRDGKDTV IGVLDIYGFE VFPVNSFEQF CINYCNEKLQ 

       430        440        450        460        470        480 
QLFIQLILKQ EQEEYEREGI TWQSVEYFNN ATIVDLVERP HRGILAVLDE ACSSAGTITD 

       490        500        510        520        530        540 
RIFLQTLDMH HRHHLHYTSR QLCPTDKTME FGRDFRIKHY AGDVTYSVEG FIDKNRDFLF 

       550        560        570        580        590        600 
QDFKRLLYNS TDPTLRAMWP DGQQDITEVT KRPLTAGTLF KNSMVALVEN LASKEPFYVR 

       610        620        630        640        650        660 
CIKPNEDKVA GKLDENHCRH QVAYLGLLEN VRVRRAGFAS RQPYSRFLLR YKMTCEYTWP 

       670        680        690        700        710        720 
NHLLGSDKAA VSALLEQHGL QGDVAFGHSK LFIRSPRTLV TLEQSRARLI PIIVLLLQKA 

       730        740        750        760        770        780 
WRGTLARWRC RRLRAIYTIM RWFRRHKVRA HLAELQRRFQ AARQPPLYGR DLVWPLPPAV 

       790        800        810        820        830        840 
LQPFQDTCHA LFCRWRARQL VKNIPPSDMP QIKAKVAAMG ALQGLRQDWG CRRAWARDYL 

       850        860        870        880        890        900 
SSATDNPTAS SLFAQRLKTL QDKDGFGAVL FSSHVRKVNR FHKIRNRALL LTDQHLYKLD 

       910        920        930        940        950        960 
PDRQYRVMRA VPLEAVTGLS VTSGGDQLVV LHARGQDDLV VCLHRSRPPL DNRVGELVGV 

       970        980        990       1000       1010 
LAAHCQGEGR TLEVRVSDCI PLSHRGVRRL ISVEPRPEQP EPDFRCARGS FTLLWPSR 

« Hide

Isoform 2 [UniParc].

Checksum: 7A80030FE7FCCE28
Show »

FASTA23026,151
Isoform 3 [UniParc].

Checksum: 1B25EB82195E4993
Show »

FASTA52559,815
Isoform 4 [UniParc].

Checksum: 6275117D9D1751C1
Show »

FASTA72282,279

References

« Hide 'large scale' references
[1]"The nucleotide sequence of a long cDNA clone isolated from human spleen."
Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 193-1018 (ISOFORM 3), VARIANT THR-489.
Tissue: Spleen.
[2]"Multiplex amplification and cloning of 5'-ends of cDNA by ligase-free recombination: preparation of full-length cDNA clones encoding motor proteins."
Yamakawa H., Kikuno R.F., Nagase T., Ohara O.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS THR-489 AND ARG-861.
Tissue: Spleen.
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The HA-2 minor histocompatibility antigen is derived from a diallelic gene encoding a novel human class I myosin protein."
Pierce R.A., Field E.D., Mutis T., Golovina T.N., Von Kap-Herr C., Wilke M., Pool J., Shabanowitz J., Pettenati M.J., Eisenlohr L.C., Hunt D.F., Goulmy E., Engelhard V.H.
J. Immunol. 167:3223-3230(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-665 (ISOFORM 4), IDENTIFICATION AS THE MINOR HISTOCOMPATIBILITY ANTIGEN HA-2, VARIANTS HA-2M MET-49 AND THR-489, CHARACTERIZATION OF VARIANT HA-2M MET-49, TISSUE SPECIFICITY.
[5]"Identification of a graft versus host disease-associated human minor histocompatibility antigen."
den Haan J.M.M., Sherman N.E., Blokland E., Huczko E., Koning F., Drijfhout J.W., Skipper J., Shabanowitz J., Hunt D.F., Engelhard V.H., Goulmy E.
Science 268:1476-1480(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 41-49 (ISOFORM 1).
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 723-1018 (ISOFORM 1), VARIANT ARG-861.
Tissue: B-cell.
[7]"Myosin 1G is an abundant class I myosin in lymphocytes whose localization at the plasma membrane depends on its ancient divergent pleckstrin homology (PH) domain (Myo1PH)."
Patino-Lopez G., Aravind L., Dong X., Kruhlak M.J., Ostap E.M., Shaw S.
J. Biol. Chem. 285:8675-8686(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-815; ARG-826; ARG-876; ARG-880; ARG-885; LYS-898; ARG-903; ARG-906; ARG-909; ARG-934; ARG-945; ARG-947 AND ARG-953.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK074050 mRNA. Translation: BAB84876.1. Sequence problems.
AK074135 mRNA. Translation: BAB84961.1.
AB290179 mRNA. Translation: BAG06733.1.
AC004847 Genomic DNA. No translation available.
AF380932 mRNA. Translation: AAK58092.1.
AF380933 mRNA. Translation: AAK58093.1.
BC015693 mRNA. Translation: AAH15693.2.
CCDSCCDS34629.1. [B0I1T2-1]
RefSeqNP_149043.2. NM_033054.2. [B0I1T2-1]
UniGeneHs.37617.

3D structure databases

ProteinModelPortalB0I1T2.
SMRB0I1T2. Positions 8-733.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122031. 6 interactions.
IntActB0I1T2. 4 interactions.
MINTMINT-7307233.
STRING9606.ENSP00000258787.

PTM databases

PhosphoSiteB0I1T2.

Proteomic databases

MaxQBB0I1T2.
PaxDbB0I1T2.
PRIDEB0I1T2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000258787; ENSP00000258787; ENSG00000136286. [B0I1T2-1]
GeneID64005.
KEGGhsa:64005.
UCSCuc003tmg.2. human. [B0I1T2-1]
uc003tmi.1. human. [B0I1T2-4]
uc003tmj.2. human. [B0I1T2-3]

Organism-specific databases

CTD64005.
GeneCardsGC07M045003.
H-InvDBHIX0006659.
HGNCHGNC:13880. MYO1G.
HPAHPA021252.
MIM613445. gene.
neXtProtNX_B0I1T2.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5022.
HOGENOMHOG000260264.
HOVERGENHBG062373.
InParanoidB0I1T2.
KOK10356.
OMAEQHGLQG.
OrthoDBEOG7V49XQ.
PhylomeDBB0I1T2.
TreeFamTF312960.

Gene expression databases

ArrayExpressB0I1T2.
BgeeB0I1T2.
CleanExHS_MYO1G.
GenevestigatorB0I1T2.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_tail_2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi64005.
NextBio65808.
PROB0I1T2.
SOURCESearch...

Entry information

Entry nameMYO1G_HUMAN
AccessionPrimary (citable) accession number: B0I1T2
Secondary accession number(s): Q8TEI9 expand/collapse secondary AC list , Q8TES2, Q96BE2, Q96RI5, Q96RI6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM