SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B0I1T2

- MYO1G_HUMAN

UniProt

B0I1T2 - MYO1G_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Unconventional myosin-Ig
Gene
MYO1G, HA2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments By similarity.
Precursor of the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi102 – 1098ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. motor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-Ig
Cleaved into the following chain:
Gene namesi
Name:MYO1G
Synonyms:HA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:13880. MYO1G.

Subcellular locationi

Cell membrane; Peripheral membrane protein
Note: Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles.1 Publication

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. myosin complex Source: UniProtKB-KW
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi815 – 8151K → A: Reduced membrane association. 1 Publication
Mutagenesisi826 – 8261R → A: Reduced membrane association. 1 Publication
Mutagenesisi876 – 8761R → A: No effect on membrane localization. 1 Publication
Mutagenesisi880 – 8801R → A: No effect on membrane localization. 1 Publication
Mutagenesisi883 – 8831K → A: No effect on membrane localization; when associated with R-885.
Mutagenesisi885 – 8851R → A: No effect on membrane localization; when associated with K-883. 1 Publication
Mutagenesisi898 – 8981K → A: Reduced membrane association. 1 Publication
Mutagenesisi903 – 9031R → A: No effect on membrane localization; when associated with R-906. 1 Publication
Mutagenesisi906 – 9061R → A: No effect on membrane localization; when associated with R-903. 1 Publication
Mutagenesisi909 – 9091R → A: No effect on membrane localization. 1 Publication
Mutagenesisi934 – 9341R → A: No effect on membrane localization. 1 Publication
Mutagenesisi945 – 9451R → A: No effect on membrane localization. 1 Publication
Mutagenesisi947 – 9471R → A: No effect on membrane localization. 1 Publication
Mutagenesisi953 – 9531R → A: No effect on membrane localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10181018Unconventional myosin-Ig
PRO_0000340316Add
BLAST
Peptidei41 – 499Minor histocompatibility antigen HA-21 Publication
PRO_0000340317

Proteomic databases

MaxQBiB0I1T2.
PaxDbiB0I1T2.
PRIDEiB0I1T2.

PTM databases

PhosphoSiteiB0I1T2.

Expressioni

Tissue specificityi

Specifically expressed in hematopoietic cells.2 Publications

Gene expression databases

ArrayExpressiB0I1T2.
BgeeiB0I1T2.
CleanExiHS_MYO1G.
GenevestigatoriB0I1T2.

Organism-specific databases

HPAiHPA021252.

Interactioni

Subunit structurei

Binds calmodulin through its IQ motifs By similarity.

Protein-protein interaction databases

BioGridi122031. 6 interactions.
IntActiB0I1T2. 4 interactions.
MINTiMINT-7307233.
STRINGi9606.ENSP00000258787.

Structurei

3D structure databases

ProteinModelPortaliB0I1T2.
SMRiB0I1T2. Positions 8-733.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 707699Myosin motor
Add
BLAST
Domaini710 – 73930IQ
Add
BLAST

Sequence similaritiesi

Contains 1 IQ domain.

Phylogenomic databases

eggNOGiCOG5022.
HOGENOMiHOG000260264.
HOVERGENiHBG062373.
InParanoidiB0I1T2.
KOiK10356.
OMAiEQHGLQG.
OrthoDBiEOG7V49XQ.
PhylomeDBiB0I1T2.
TreeFamiTF312960.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_tail_2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: B0I1T2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN     50
PYQELPLYGP EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI 100
SGESGAGKTE ASKHIMQYIA AVTNPSQRAE VERVKDVLLK STCVLEAFGN 150
ARTNRNHNSS RFGKYMDINF DFKGDPIGGH IHSYLLEKSR VLKQHVGERN 200
FHAFYQLLRG SEDKQLHELH LERNPAVYNF THQGAGLNMT VHSALDSDEQ 250
SHQAVTEAMR VIGFSPEEVE SVHRILAAIL HLGNIEFVET EEGGLQKEGL 300
AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY 350
ARDACAKAVY QRLFEWVVNR INSVMEPRGR DPRRDGKDTV IGVLDIYGFE 400
VFPVNSFEQF CINYCNEKLQ QLFIQLILKQ EQEEYEREGI TWQSVEYFNN 450
ATIVDLVERP HRGILAVLDE ACSSAGTITD RIFLQTLDMH HRHHLHYTSR 500
QLCPTDKTME FGRDFRIKHY AGDVTYSVEG FIDKNRDFLF QDFKRLLYNS 550
TDPTLRAMWP DGQQDITEVT KRPLTAGTLF KNSMVALVEN LASKEPFYVR 600
CIKPNEDKVA GKLDENHCRH QVAYLGLLEN VRVRRAGFAS RQPYSRFLLR 650
YKMTCEYTWP NHLLGSDKAA VSALLEQHGL QGDVAFGHSK LFIRSPRTLV 700
TLEQSRARLI PIIVLLLQKA WRGTLARWRC RRLRAIYTIM RWFRRHKVRA 750
HLAELQRRFQ AARQPPLYGR DLVWPLPPAV LQPFQDTCHA LFCRWRARQL 800
VKNIPPSDMP QIKAKVAAMG ALQGLRQDWG CRRAWARDYL SSATDNPTAS 850
SLFAQRLKTL QDKDGFGAVL FSSHVRKVNR FHKIRNRALL LTDQHLYKLD 900
PDRQYRVMRA VPLEAVTGLS VTSGGDQLVV LHARGQDDLV VCLHRSRPPL 950
DNRVGELVGV LAAHCQGEGR TLEVRVSDCI PLSHRGVRRL ISVEPRPEQP 1000
EPDFRCARGS FTLLWPSR 1018
Length:1,018
Mass (Da):116,442
Last modified:January 11, 2011 - v2
Checksum:i3EB4ACC3D99A86E9
GO
Isoform 2 (identifier: B0I1T2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     207-230: LLRGSEDKQLHELHLERNPAVYNF → VSPEGKGRWKNGVGKGRAASWTSL
     231-1018: Missing.

Note: No experimental confirmation available.

Show »
Length:230
Mass (Da):26,151
Checksum:i7A80030FE7FCCE28
GO
Isoform 3 (identifier: B0I1T2-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     526-1018: Missing.

Note: No experimental confirmation available.

Show »
Length:525
Mass (Da):59,815
Checksum:i1B25EB82195E4993
GO
Isoform 4 (identifier: B0I1T2-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     652-722: KMTCEYTWPN...IVLLLQKAWR → WHLTPITPWA...TGGNMGMRAV
     723-1018: Missing.

Note: No experimental confirmation available.

Show »
Length:722
Mass (Da):82,279
Checksum:i6275117D9D1751C1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti49 – 491V → M in allele HA-2M; the HA-2V allele constitute the HA-2 epitope while HA-2M is not recognized by HA-2 cytotoxic T lymphocytes. 1 Publication
Corresponds to variant rs61739531 [ dbSNP | Ensembl ].
VAR_044013
Natural varianti489 – 4891M → T.3 Publications
Corresponds to variant rs3735485 [ dbSNP | Ensembl ].
VAR_044014
Natural varianti798 – 7981R → Q.
Corresponds to variant rs2107737 [ dbSNP | Ensembl ].
VAR_050212
Natural varianti861 – 8611Q → R.2 Publications
Corresponds to variant rs7792760 [ dbSNP | Ensembl ].
VAR_044015

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei207 – 23024LLRGS…AVYNF → VSPEGKGRWKNGVGKGRAAS WTSL in isoform 2.
VSP_034208Add
BLAST
Alternative sequencei231 – 1018788Missing in isoform 2.
VSP_034209Add
BLAST
Alternative sequencei526 – 1018493Missing in isoform 3.
VSP_034210Add
BLAST
Alternative sequencei652 – 72271KMTCE…QKAWR → WHLTPITPWAIVPVWSPRGR SRGSPNSTSQTSIQAGTSTL LASRHQNIWEDMCVSTCMWG HTGGNMGMRAV in isoform 4.
VSP_034211Add
BLAST
Alternative sequencei723 – 1018296Missing in isoform 4.
VSP_034212Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti369 – 3691N → K in BAB84961. 1 Publication
Sequence conflicti377 – 3771P → L in AAK58092. 1 Publication
Sequence conflicti377 – 3771P → L in AAK58093. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK074050 mRNA. Translation: BAB84876.1. Sequence problems.
AK074135 mRNA. Translation: BAB84961.1.
AB290179 mRNA. Translation: BAG06733.1.
AC004847 Genomic DNA. No translation available.
AF380932 mRNA. Translation: AAK58092.1.
AF380933 mRNA. Translation: AAK58093.1.
BC015693 mRNA. Translation: AAH15693.2.
CCDSiCCDS34629.1. [B0I1T2-1]
RefSeqiNP_149043.2. NM_033054.2. [B0I1T2-1]
UniGeneiHs.37617.

Genome annotation databases

EnsembliENST00000258787; ENSP00000258787; ENSG00000136286. [B0I1T2-1]
GeneIDi64005.
KEGGihsa:64005.
UCSCiuc003tmg.2. human. [B0I1T2-1]
uc003tmi.1. human. [B0I1T2-4]
uc003tmj.2. human. [B0I1T2-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK074050 mRNA. Translation: BAB84876.1 . Sequence problems.
AK074135 mRNA. Translation: BAB84961.1 .
AB290179 mRNA. Translation: BAG06733.1 .
AC004847 Genomic DNA. No translation available.
AF380932 mRNA. Translation: AAK58092.1 .
AF380933 mRNA. Translation: AAK58093.1 .
BC015693 mRNA. Translation: AAH15693.2 .
CCDSi CCDS34629.1. [B0I1T2-1 ]
RefSeqi NP_149043.2. NM_033054.2. [B0I1T2-1 ]
UniGenei Hs.37617.

3D structure databases

ProteinModelPortali B0I1T2.
SMRi B0I1T2. Positions 8-733.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122031. 6 interactions.
IntActi B0I1T2. 4 interactions.
MINTi MINT-7307233.
STRINGi 9606.ENSP00000258787.

PTM databases

PhosphoSitei B0I1T2.

Proteomic databases

MaxQBi B0I1T2.
PaxDbi B0I1T2.
PRIDEi B0I1T2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000258787 ; ENSP00000258787 ; ENSG00000136286 . [B0I1T2-1 ]
GeneIDi 64005.
KEGGi hsa:64005.
UCSCi uc003tmg.2. human. [B0I1T2-1 ]
uc003tmi.1. human. [B0I1T2-4 ]
uc003tmj.2. human. [B0I1T2-3 ]

Organism-specific databases

CTDi 64005.
GeneCardsi GC07M045003.
H-InvDB HIX0006659.
HGNCi HGNC:13880. MYO1G.
HPAi HPA021252.
MIMi 613445. gene.
neXtProti NX_B0I1T2.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5022.
HOGENOMi HOG000260264.
HOVERGENi HBG062373.
InParanoidi B0I1T2.
KOi K10356.
OMAi EQHGLQG.
OrthoDBi EOG7V49XQ.
PhylomeDBi B0I1T2.
TreeFami TF312960.

Miscellaneous databases

GenomeRNAii 64005.
NextBioi 65808.
PROi B0I1T2.
SOURCEi Search...

Gene expression databases

ArrayExpressi B0I1T2.
Bgeei B0I1T2.
CleanExi HS_MYO1G.
Genevestigatori B0I1T2.

Family and domain databases

InterProi IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_tail_2.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view ]
PRINTSi PR00193. MYOSINHEAVY.
SMARTi SM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 193-1018 (ISOFORM 3), VARIANT THR-489.
    Tissue: Spleen.
  2. "Multiplex amplification and cloning of 5'-ends of cDNA by ligase-free recombination: preparation of full-length cDNA clones encoding motor proteins."
    Yamakawa H., Kikuno R.F., Nagase T., Ohara O.
    Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS THR-489 AND ARG-861.
    Tissue: Spleen.
  3. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The HA-2 minor histocompatibility antigen is derived from a diallelic gene encoding a novel human class I myosin protein."
    Pierce R.A., Field E.D., Mutis T., Golovina T.N., Von Kap-Herr C., Wilke M., Pool J., Shabanowitz J., Pettenati M.J., Eisenlohr L.C., Hunt D.F., Goulmy E., Engelhard V.H.
    J. Immunol. 167:3223-3230(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-665 (ISOFORM 4), IDENTIFICATION AS THE MINOR HISTOCOMPATIBILITY ANTIGEN HA-2, VARIANTS HA-2M MET-49 AND THR-489, CHARACTERIZATION OF VARIANT HA-2M MET-49, TISSUE SPECIFICITY.
  5. "Identification of a graft versus host disease-associated human minor histocompatibility antigen."
    den Haan J.M.M., Sherman N.E., Blokland E., Huczko E., Koning F., Drijfhout J.W., Skipper J., Shabanowitz J., Hunt D.F., Engelhard V.H., Goulmy E.
    Science 268:1476-1480(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-49 (ISOFORM 1).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 723-1018 (ISOFORM 1), VARIANT ARG-861.
    Tissue: B-cell.
  7. "Myosin 1G is an abundant class I myosin in lymphocytes whose localization at the plasma membrane depends on its ancient divergent pleckstrin homology (PH) domain (Myo1PH)."
    Patino-Lopez G., Aravind L., Dong X., Kruhlak M.J., Ostap E.M., Shaw S.
    J. Biol. Chem. 285:8675-8686(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-815; ARG-826; ARG-876; ARG-880; ARG-885; LYS-898; ARG-903; ARG-906; ARG-909; ARG-934; ARG-945; ARG-947 AND ARG-953.

Entry informationi

Entry nameiMYO1G_HUMAN
AccessioniPrimary (citable) accession number: B0I1T2
Secondary accession number(s): Q8TEI9
, Q8TES2, Q96BE2, Q96RI5, Q96RI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi