B0G172 (PGTB2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable geranylgeranyl transferase type-2 subunit beta
EC=2.5.1.60

Alternative name(s):
Geranylgeranyl transferase type II subunit beta
Short name=GGTase-II-beta
Rab geranyl-geranyltransferase subunit beta
Short name=Rab GG transferase beta
Short name=Rab GGTase beta
Rab geranylgeranyltransferase subunit beta
Type II protein geranyl-geranyltransferase subunit beta

Gene names

Name:	rabggtb
ORF Names:	DDB_G0290671

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal By similarity.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Cofactor	Binds 1 zinc ion per dimer By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 6 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein geranylgeranylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	Rab-protein geranylgeranyltransferase complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	Rab geranylgeranyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Probable geranylgeranyl transferase type-2 subunit beta		PRO_0000331291
Regions
	Repeat	24 – 65	42	PFTB 1
	Repeat	72 – 113	42	PFTB 2
	Repeat	120 – 161	42	PFTB 3
	Repeat	168 – 209	42	PFTB 4
	Repeat	216 – 257	42	PFTB 5
	Repeat	264 – 306	43	PFTB 6
	Compositional bias	10 – 15	6	Poly-Thr
Sites
	Metal binding	242	1	Zinc By similarity
	Metal binding	244	1	Zinc By similarity
	Metal binding	294	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0G172 [UniParc]. Last modified March 18, 2008. Version 1. Checksum: C5741A1FB950F662	FASTA	339	38,012
	10 20 30 40 50 60 MTDNINKEST TTTTTIDHTT NLLIDKHVEY IVKLGSKKDS FEYWVTEHIR MNGMYWGLSS 70 80 90 100 110 120 LYLLKSLDKL DKNEVIQWLL SCQKSNGGFG GNTSHDDHLL STLSAVQILI QYDALDKIDI 130 140 150 160 170 180 NSVVDYVVKL QREDGSFVGD QWGEVDTRFS YAAIMCLSLL KSLDKINCEK AVEYILSCQN 190 200 210 220 230 240 FDGGFGSIPG AESHAGQIFT CVGALSILNE INKIDIDKLG WWLSERQLPN GGLNGRPEKS 250 260 270 280 290 300 SDVCYSWWVL SALSAIDRLH WIDNDKLKSY ILKCQDNETG GIADKPGDIP DVFHTFFGIC 310 320 330 GLSLMGYFKD QIESIDPVYA LGTKTLQKLG LNLPWNKNL

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000164 Genomic DNA. Translation: EDR41036.1.
RefSeq	XP_001733035.1. XM_001732983.1.
3D structure databases
ProteinModelPortal	B0G172.
SMR	B0G172. Positions 18-331.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0219584.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233949; DDB0233949; DDB_G0290671.
GeneID	8627741.
KEGG	ddi:DDB_G0290671.
Organism-specific databases
dictyBase	DDB_G0290671. rabggtb.
Phylogenomic databases
eggNOG	COG5029.
KO	K05956.
OMA	ESHAGLI.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR026873. Ptb1. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
PANTHER	PTHR11774:SF5. PTHR11774:SF5. 1 hit.
Pfam	PF00432. Prenyltrans. 2 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PGTB2_DICDI

Accession

Primary (citable) accession number: B0G172

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)