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B0CRL4

- MAP2_LACBS

UniProt

B0CRL4 - MAP2_LACBS

Protein

Methionine aminopeptidase 2

Gene

LACBIDRAFT_242662

Organism
Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei111 – 1111SubstrateUniRule annotation
    Metal bindingi131 – 1311Divalent metal cation 1UniRule annotation
    Metal bindingi142 – 1421Divalent metal cation 1UniRule annotation
    Metal bindingi142 – 1421Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi211 – 2111Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei219 – 2191SubstrateUniRule annotation
    Metal bindingi244 – 2441Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi339 – 3391Divalent metal cation 1UniRule annotation
    Metal bindingi339 – 3391Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2UniRule annotation
    Short name:
    MetAP 2UniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    ORF Names:LACBIDRAFT_242662
    OrganismiLaccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor)
    Taxonomic identifieri486041 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesTricholomataceaeLaccaria
    ProteomesiUP000001194: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Methionine aminopeptidase 2PRO_0000407653Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi29883.JGI242662.

    Structurei

    3D structure databases

    ProteinModelPortaliB0CRL4.
    SMRiB0CRL4. Positions 23-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0024.
    KOiK01265.
    OrthoDBiEOG7BGHW3.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B0CRL4-1 [UniParc]FASTAAdd to Basket

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    MSACTSFLRV AGDKVNLSFS SNSWRTTSEE KRYDERMANE DPEKTYQSIR    50
    RAAEVHRQVR QHARRHIRPG MTMTEIANNI EDGTRALVEE DGLLSGVGFP 100
    TGLSLNNCAA HYTPNAGDTT VLQKGDVLKV DIGVHVKGRI ADSAFTLTWE 150
    PTYNKLLEAV KAATDTGIRE SGIDARLGEI AGAIQETMES YEVEVNGTVY 200
    PVKPIENLSG HSINPYQIHG GKSILLVKND DQTKMEEGEY FAIETFGSTG 250
    RGRIVESGEV SHYARRMDAP HVPLRLTSAK TLLKSINKNF GTLPFCRRYL 300
    DRAGESKYLL ALNHLVGQGI VQDYPPLCDQ RGSMTAQFEH TILLRPTVKE 350
    VVTRGDDY 358
    Length:358
    Mass (Da):39,747
    Last modified:February 26, 2008 - v1
    Checksum:i4EE375B300D588AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS547091 Genomic DNA. Translation: EDR15225.1.
    RefSeqiXP_001873433.1. XM_001873398.1.

    Genome annotation databases

    GeneIDi6069222.
    KEGGilbc:LACBIDRAFT_242662.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS547091 Genomic DNA. Translation: EDR15225.1 .
    RefSeqi XP_001873433.1. XM_001873398.1.

    3D structure databases

    ProteinModelPortali B0CRL4.
    SMRi B0CRL4. Positions 23-358.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 29883.JGI242662.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6069222.
    KEGGi lbc:LACBIDRAFT_242662.

    Phylogenomic databases

    eggNOGi COG0024.
    KOi K01265.
    OrthoDBi EOG7BGHW3.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis."
      Martin F., Aerts A., Ahren D., Brun A., Danchin E.G.J., Duchaussoy F., Gibon J., Kohler A., Lindquist E., Pereda V., Salamov A., Shapiro H.J., Wuyts J., Blaudez D., Buee M., Brokstein P., Canbaeck B., Cohen D.
      , Courty P.E., Coutinho P.M., Delaruelle C., Detter J.C., Deveau A., DiFazio S., Duplessis S., Fraissinet-Tachet L., Lucic E., Frey-Klett P., Fourrey C., Feussner I., Gay G., Grimwood J., Hoegger P.J., Jain P., Kilaru S., Labbe J., Lin Y.C., Legue V., Le Tacon F., Marmeisse R., Melayah D., Montanini B., Muratet M., Nehls U., Niculita-Hirzel H., Oudot-Le Secq M.P., Peter M., Quesneville H., Rajashekar B., Reich M., Rouhier N., Schmutz J., Yin T., Chalot M., Henrissat B., Kuees U., Lucas S., Van de Peer Y., Podila G.K., Polle A., Pukkila P.J., Richardson P.M., Rouze P., Sanders I.R., Stajich J.E., Tunlid A., Tuskan G., Grigoriev I.V.
      Nature 452:88-92(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S238N-H82 / ATCC MYA-4686.

    Entry informationi

    Entry nameiMAP2_LACBS
    AccessioniPrimary (citable) accession number: B0CRL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3