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B0CRC2 (PMIP_LACBS) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial intermediate peptidase

Short name=MIP
EC=3.4.24.59
Alternative name(s):
Octapeptidyl aminopeptidase
Gene names
Name:OCT1
Synonyms:alpha-fg, MIP
ORF Names:LACBIDRAFT_181082
OrganismLaccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor) [Complete proteome]
Taxonomic identifier486041 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesTricholomataceaeLaccaria

Protein attributes

Sequence length772 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.

Catalytic activity

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactor

Binds 1 zinc ion By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the peptidase M3 family.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4242Mitochondrion Potential
Chain43 – 772730Mitochondrial intermediate peptidase
PRO_0000338585

Regions

Compositional bias485 – 4884Poly-Asp

Sites

Active site5571 By similarity
Metal binding5561Zinc; catalytic By similarity
Metal binding5601Zinc; catalytic By similarity
Metal binding5631Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
B0CRC2 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: 8E4B2FA8D81D1F7F

FASTA77286,856
        10         20         30         40         50         60 
MFANSARNAL KKRPQNLQPF RFQGCLFSKR ANRPLTTKVQ HLIYASVDDK ALVTLFDQPR 

        70         80         90        100        110        120 
STLRSPFATT GLFGHPSLTH PRALISLADA TVVRAQLLTD RILRARESRT ELLRVVKNLD 

       130        140        150        160        170        180 
RLSDMLCGVI DLAELVRNAH PDRSWVDAAN RAYETLCEFM NVLNTHVGLY EVLKAVLSDP 

       190        200        210        220        230        240 
SIVKTLGPEA HQTALIFWRD FEKSAIDLPA EQRKKFVSLS SDILVLGRQF LEGANAPRPP 

       250        260        270        280        290        300 
ASIKPSQLSG LKDKGMGVRL QLQAQFTQRD LQVYPGSLQA QMIMRSAPEE EPRRQVYLAA 

       310        320        330        340        350        360 
NSSTPQQIEV LEKLLRTRAE LARLVGRDSF AHMTLDDKMA KTPDNVWNFL DALMDHTKPF 

       370        380        390        400        410        420 
ARRALHTLSE RKQLHHGTSS LPIIQAWDRD FYCPPDPPAP PIPLPPLTLG TVFMGLSRLF 

       430        440        450        460        470        480 
QHMYGISLRP ADSASGEVWH TDVQKLEVVD QDQGIIGWIY ADLFARRGKA SGAAHYTVRC 

       490        500        510        520        530        540 
SRRTDDDDES SDGTVEGAEL LIYESQEFEA VKRHRLPNQD GIYQLPLVVL LCEFARPTPS 

       550        560        570        580        590        600 
KGPTVLEWHE VLTLFHEMGH AMHSMIGRTE YQNVAGTRCA TDFVEFPSIL MEHFLNSPTV 

       610        620        630        640        650        660 
LSLFDVDGTS TVRHIGNHHN DPCHFIDTYS QILLAAVDQV YHSPAVLDPT FDSTAELAKV 

       670        680        690        700        710        720 
HNTRGLIPYV PGTSFQTQFG HLYGYGATYY SYLLDRAIAS RVWRNVFLDD PLDRETGEKF 

       730        740        750        760        770 
KCEVLRFGGG KDPWKMVSAL LDVPELSTGD AEAMREIGRW KINSEIGVHG RH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS547091 Genomic DNA. Translation: EDR15793.1.
RefSeqXP_001874001.1. XM_001873966.1.

3D structure databases

ProteinModelPortalB0CRC2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING29883.JGI181082.

Protein family/group databases

MEROPSM03.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6069018.
KEGGlbc:LACBIDRAFT_181082.

Phylogenomic databases

eggNOGCOG0339.
KOK01410.
OrthoDBEOG71GB4R.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 2 hits.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMIP_LACBS
AccessionPrimary (citable) accession number: B0CRC2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: February 26, 2008
Last modified: November 13, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries