Reviewed, UniProtKB/Swiss-Prot B0CQN7 (NCB5R_LACBS)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	MCR1.1
ORF Names:	LACBIDRAFT_300832

Organism

Laccaria bicolor (strain S238N-H82) (Bicoloured deceiver) (Laccaria laccata var. bicolor)

Taxonomic identifier

486041 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Tricholomataceae › Laccaria

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	NADH-cytochrome b5 reductase 1		PRO_0000330155
Regions
	Transmembrane	29 – 49	21	Potential
	Domain	64 – 167	104	FAD-binding FR-type
	Nucleotide binding	147 – 162	16	FAD By similarity
	Nucleotide binding	173 – 205	33	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0CQN7-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 22FB3E946AF9C4E6	FASTA	308	33,656
	10 20 30 40 50 60 MSGRVEVENI PGQVANLLKN VTAGDLLNVA SSPAFLVAAA AIVIAAAFYS KVFNSTRPKP 70 80 90 100 110 120 LDPSIWKEFP LQKKNQVSPN TAIYTFKLPH AEDVLGLPIG QHISVSADIN GKNIVRSYTP 130 140 150 160 170 180 ISRQNARGRF ELIIKTYEKG NISRHVASLK IGDTLRVKGP KGNFKYTPGL TAHLGMIAGG 190 200 210 220 230 240 TGLAPMIQIV RAILQNPPDR TNITLIYANV NEEDILLRAE LDALAMGYES RFNLFYVLNN 250 260 270 280 290 300 PPSGWTGGVG FVTKEHIKDL LPNPNESNSK ILICGPPPMV TAMKKNLEEI KYPVPNTISK LDDKVFVF

References

[1]

"The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis." Martin F., Aerts A., Ahren D., Brun A., Danchin E.G.J., Duchaussoy F., Gibon J., Kohler A., Lindquist E., Pereda V., Salamov A., Shapiro H.J., Wuyts J., Blaudez D., Buee M., Brokstein P., Canbaeck B., Cohen D. , Courty P.E., Coutinho P.M., Delaruelle C., Detter J.C., Deveau A., DiFazio S., Duplessis S., Fraissinet-Tachet L., Lucic E., Frey-Klett P., Fourrey C., Feussner I., Gay G., Grimwood J., Hoegger P.J., Jain P., Kilaru S., Labbe J., Lin Y.C., Legue V., Le Tacon F., Marmeisse R., Melayah D., Montanini B., Muratet M., Nehls U., Niculita-Hirzel H., Oudot-Le Secq M.P., Peter M., Quesneville H., Rajashekar B., Reich M., Rouhier N., Schmutz J., Yin T., Chalot M., Henrissat B., Kuees U., Lucas S., Van de Peer Y., Podila G.K., Polle A., Pukkila P.J., Richardson P.M., Rouze P., Sanders I.R., Stajich J.E., Tunlid A., Tuskan G., Grigoriev I.V. Nature 452:88-92(2008) [PubMed: 18322534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS547091 Genomic DNA. Translation: EDR15673.1.
RefSeq	XP_001873881.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6069923.
KEGG	lbc:LACBIDRAFT_300832.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_LACBS

Accession

Primary (citable) accession number: B0CQN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)