Reviewed, UniProtKB/Swiss-Prot B0CL36 (PUR5_BRUSI)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	BSUIS_A0741

Organism

Brucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]

Taxonomic identifier

470137 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity HAMAP MF_00741.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_1000083452

Sequences

	Sequence		Length	Mass (Da)
	B0CL36-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 594BAB33E3F569F7	FASTA	359	37,447
	10 20 30 40 50 60 MTMENKPAGQ NGLTYAQAGV DIDAGNLMVE KIKPLVRSTR RPGADGEIGG FGGLFDLKAA 70 80 90 100 110 120 GFKDPVLVAA NDGVGTKLKI AIDADIHDTV GIDLVAMCVN DLVVQGAEPL FFLDYYATGK 130 140 150 160 170 180 LSPDQGVAIV SGIAEGCRQA GCALIGGETA EMPGMYRDGD YDLAGFAVGA AERDRLLPRG 190 200 210 220 230 240 DIAEGDIILG LASSGVHSNG FSLVRRIVEL SGLGWKSQAP FQPGATLGEA LLTPTRIYVK 250 260 270 280 290 300 PLLAAIRACD GIKALAHITG GGFPDNIPRV LPKGLAAEID LPAIAVPPVF SWLAKTGNVE 310 320 330 340 350 PNEMLRTFNC GIGMIAVVNP AKVDEVIAAL AAEGEKVVTL GRMTRREKDG VIYKGQLAL

References

[1]

"Brucella suis ATCC 23445 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000911 Genomic DNA. Translation: ABY37816.1.
RefSeq	YP_001627386.1.
3D structure databases
SMR	B0CL36. Positions 12-349, 53-355.
ModBase	Search...
Genome annotation databases
GeneID	5838786.
GenomeReviews	Gene locus BSUIS_A0741 in contig CP000911_GR.
KEGG	bmt:BSUIS_A0741.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG531222.
OMA	HYDLAGF.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BRUSI

Accession

Primary (citable) accession number: B0CL36

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)