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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Brucella suis (strain ATCC 23445 / NCTC 10510)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotationSAAS annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotationSAAS annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi263 – 2631Manganese 2UniRule annotation
Metal bindingi268 – 2681Manganese 1UniRule annotation
Metal bindingi268 – 2681Manganese 2UniRule annotation
Active sitei275 – 2751UniRule annotation
Metal bindingi286 – 2861Manganese 2UniRule annotation
Metal bindingi345 – 3451Manganese 1UniRule annotation
Metal bindingi347 – 3471Manganese 1UniRule annotation
Metal bindingi347 – 3471Manganese 2UniRule annotation
Active sitei349 – 3491UniRule annotation

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

AminopeptidaseUniRule annotationSAAS annotation, Hydrolase, Protease

Keywords - Ligandi

ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBSUI470137:GJIC-710-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation
Alternative name(s):
Leucine aminopeptidaseUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotationImported
Ordered Locus Names:BSUIS_A0717Imported
OrganismiBrucella suis (strain ATCC 23445 / NCTC 10510)Imported
Taxonomic identifieri470137 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000008545: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi470137.BSUIS_A0717.

Structurei

3D structure databases

ProteinModelPortaliB0CL13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiGGMEDMK.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0CL13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRPSISFS EFEVPQKGVA VVLVAKGGGF ADEAAKAVGG AEKIARIADI
60 70 80 90 100
SGFTGALGKT AEAIETTPAG VEKIVLVGVG EPGKLGNDDW LKIGGAAFSQ
110 120 130 140 150
IGNAERVTLT LALPETTIAG DEAADVALGM VLRSYKFDRY KTRKSEENGE
160 170 180 190 200
PKHAAKITIC VADTHSARKT FEVAEAVADG VIQARNLVNE PANILGPVEF
210 220 230 240 250
AEEAEKLEKL GVKVEVLGEK ELKKLGMGAL LGVAQGSVRP PRLVVMEWHG
260 270 280 290 300
AKGKEKPIAF VGKGVVFDTG GISIKPAANM EDMKGDMGGA AAVTGLMRAL
310 320 330 340 350
AGRKAKVNAI GVIGLVENMP DGNAQRPGDI VTSMSGQTIE VINTDAEGRL
360 370 380 390 400
VLADALHYTN DRFKPRFIIN LATLTGAVMV ALGQYHAGLF SNDDELADQL
410 420 430 440 450
YDAGQSTGEK LWRLPLGTEY DKMIDSKFAD MKNSAGRYGG SITAAQFLKR
460 470 480 490
FVGETPWAHL DVAGTAMGSP ANEYNQSWAS GFGVRLLDRL VRDQFES
Length:497
Mass (Da):52,576
Last modified:February 26, 2008 - v1
Checksum:i287DD9E0C90DC3AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000911 Genomic DNA. Translation: ABY37793.1.
RefSeqiYP_001627363.1. NC_010169.1.

Genome annotation databases

EnsemblBacteriaiABY37793; ABY37793; BSUIS_A0717.
GeneIDi5839178.
KEGGibmt:BSUIS_A0717.
PATRICi17868972. VBIBruSui83806_2289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000911 Genomic DNA. Translation: ABY37793.1.
RefSeqiYP_001627363.1. NC_010169.1.

3D structure databases

ProteinModelPortaliB0CL13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi470137.BSUIS_A0717.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY37793; ABY37793; BSUIS_A0717.
GeneIDi5839178.
KEGGibmt:BSUIS_A0717.
PATRICi17868972. VBIBruSui83806_2289.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiGGMEDMK.
OrthoDBiEOG6FV8B3.

Enzyme and pathway databases

BioCyciBSUI470137:GJIC-710-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23445 / NCTC 10510Imported.

Entry informationi

Entry nameiB0CL13_BRUSI
AccessioniPrimary (citable) accession number: B0CL13
Entry historyi
Integrated into UniProtKB/TrEMBL: February 26, 2008
Last sequence update: February 26, 2008
Last modified: March 4, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.