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B0CL13 (B0CL13_BRUSI) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase HAMAP-Rule MF_00181
EC=3.4.11.1 HAMAP-Rule MF_00181
Alternative name(s):
Leucine aminopeptidase HAMAP-Rule MF_00181
Leucyl aminopeptidase HAMAP-Rule MF_00181
Gene names
Name:pepA HAMAP-Rule MF_00181 EMBL ABY37793.1
Ordered Locus Names:BSUIS_A0717
OrganismBrucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]
Taxonomic identifier470137 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181 SAAS SAAS011356

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181 SAAS SAAS011356

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. HAMAP-Rule MF_00181

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family. HAMAP-Rule MF_00181

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2751 By similarity HAMAP-Rule MF_00181
Active site3491 By similarity HAMAP-Rule MF_00181
Metal binding2631Manganese 2 By similarity HAMAP-Rule MF_00181
Metal binding2681Manganese 1 By similarity HAMAP-Rule MF_00181
Metal binding2681Manganese 2 By similarity HAMAP-Rule MF_00181
Metal binding2861Manganese 2 By similarity HAMAP-Rule MF_00181
Metal binding3451Manganese 1 By similarity HAMAP-Rule MF_00181
Metal binding3471Manganese 1 By similarity HAMAP-Rule MF_00181
Metal binding3471Manganese 2 By similarity HAMAP-Rule MF_00181

Sequences

Sequence LengthMass (Da)Tools
B0CL13 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: 287DD9E0C90DC3AC

FASTA49752,576
        10         20         30         40         50         60 
MSKRPSISFS EFEVPQKGVA VVLVAKGGGF ADEAAKAVGG AEKIARIADI SGFTGALGKT 

        70         80         90        100        110        120 
AEAIETTPAG VEKIVLVGVG EPGKLGNDDW LKIGGAAFSQ IGNAERVTLT LALPETTIAG 

       130        140        150        160        170        180 
DEAADVALGM VLRSYKFDRY KTRKSEENGE PKHAAKITIC VADTHSARKT FEVAEAVADG 

       190        200        210        220        230        240 
VIQARNLVNE PANILGPVEF AEEAEKLEKL GVKVEVLGEK ELKKLGMGAL LGVAQGSVRP 

       250        260        270        280        290        300 
PRLVVMEWHG AKGKEKPIAF VGKGVVFDTG GISIKPAANM EDMKGDMGGA AAVTGLMRAL 

       310        320        330        340        350        360 
AGRKAKVNAI GVIGLVENMP DGNAQRPGDI VTSMSGQTIE VINTDAEGRL VLADALHYTN 

       370        380        390        400        410        420 
DRFKPRFIIN LATLTGAVMV ALGQYHAGLF SNDDELADQL YDAGQSTGEK LWRLPLGTEY 

       430        440        450        460        470        480 
DKMIDSKFAD MKNSAGRYGG SITAAQFLKR FVGETPWAHL DVAGTAMGSP ANEYNQSWAS 

       490 
GFGVRLLDRL VRDQFES

« Hide

References

[1]"Brucella suis ATCC 23445 whole genome shotgun sequencing project."
Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. expand/collapse author list , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23445 / NCTC 10510.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000911 Genomic DNA. Translation: ABY37793.1.
RefSeqYP_001627363.1. NC_010169.1.

3D structure databases

ProteinModelPortalB0CL13.
ModBaseSearch...

Protein-protein interaction databases

STRING470137.BSUIS_A0717.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY37793; ABY37793; BSUIS_A0717.
GeneID5839178.
KEGGbmt:BSUIS_A0717.
PATRIC17868972. VBIBruSui83806_2289.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0260.
HOGENOMHOG000243129.
KOK01255.
OMANMHLMRY.
ProtClustDBPRK00913.

Enzyme and pathway databases

BioCycBSUI470137:GJIC-710-MONOMER.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERPTHR11963:SF3. PTHR11963:SF3. 1 hit.
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB0CL13_BRUSI
AccessionPrimary (citable) accession number: B0CL13
Entry history
Integrated into UniProtKB/TrEMBL: February 26, 2008
Last sequence update: February 26, 2008
Last modified: May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info