Reviewed, UniProtKB/Swiss-Prot B0CK15 (PNP_BRUSI)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	BSUIS_A2006

Organism

Brucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]

Taxonomic identifier

470137 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_1000087985
Regions
	Domain	555 – 614	60	KH
	Domain	624 – 692	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	B0CK15-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 61E6696AD66B29F7	FASTA	714	77,730
	10 20 30 40 50 60 MFNTHKVEIE WGGRPLTLET GKIARQADGA VLATYGETAV LATVVSAKEP KPGQDFFPLT 70 80 90 100 110 120 VNYQEKTYAA GKIPGGYFKR EGRPSENETL VSRLIDRPIR PLFVDGYKND TQVVITVLQH 130 140 150 160 170 180 DLENNPDILS MVAASAALTI SGVPFMGPIS GARVGYIDGE YVLNPNIDEM PESKLDLVVA 190 200 210 220 230 240 GTSEAVLMVE SEAQELPEDV MLGAVMFGHK SFQPVIDAII KLAEVAAKEP RDFQPEDLSE 250 260 270 280 290 300 LEAKVLAVVE NDLREAYKIT EKQARYAAVD AAKAKAKEHF FPEGVEETEM SAEQFATIFK 310 320 330 340 350 360 HLQAKIVRWN ILDTGNRIDG RDLSTVRPIV SEVGILPRTH GSALFTRGET QAIVVATLGT 370 380 390 400 410 420 GEDEQMIDAL TGTYKESFML HYNFPPYSVG ETGRMGSPGR REIGHGKLAW RAIHPMLPAA 430 440 450 460 470 480 EQFPYTIRAV SEITESNGSS SMATVCGTSL ALMDAGVPIV RPVAGIAMGL IKEGERFAVL 490 500 510 520 530 540 SDILGDEDHL GDMDFKVAGT EFGITSLQMD IKIDGITEEI MKVALEQAKG GRVHILGEMA 550 560 570 580 590 600 KAISSSRAEL GEFAPRIEVM NIPTDKIRDV IGSGGKVIRE IVEKTGAKIN IEDDGTVKIA 610 620 630 640 650 660 SSNGKEIEAA KKWIHSIVAE PEVGEIYEGT VVKTADFGAF VNFFGPRDGL VHISQLAADR 670 680 690 700 710 VAKTTDVVKE GQKVWVKLMG FDERGKVRLS MKVVDQETGK EIVAEKKKEE VDAE

References

[1]

"Brucella suis ATCC 23445 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000911 Genomic DNA. Translation: ABY39016.1.
RefSeq	YP_001628587.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5838194.
GenomeReviews	Gene locus BSUIS_A2006 in contig CP000911_GR.
KEGG	bmt:BSUIS_A2006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0CK15. LHISEIR.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_BRUSI

Accession

Primary (citable) accession number: B0CK15

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)