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B0CK15

- PNP_BRUSI

UniProt

B0CK15 - PNP_BRUSI

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Brucella suis (strain ATCC 23445 / NCTC 10510)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi488 – 4881MagnesiumUniRule annotation
    Metal bindingi494 – 4941MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciBSUI470137:GJIC-1997-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:BSUIS_A2006
    OrganismiBrucella suis (strain ATCC 23445 / NCTC 10510)
    Taxonomic identifieri470137 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000008545: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 714714Polyribonucleotide nucleotidyltransferasePRO_1000087985Add
    BLAST

    Proteomic databases

    PRIDEiB0CK15.

    Interactioni

    Protein-protein interaction databases

    STRINGi470137.BSUIS_A2006.

    Structurei

    3D structure databases

    ProteinModelPortaliB0CK15.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini555 – 61460KHUniRule annotationAdd
    BLAST
    Domaini624 – 69269S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218327.
    KOiK00962.
    OMAiHGNFKSN.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0CK15-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNTHKVEIE WGGRPLTLET GKIARQADGA VLATYGETAV LATVVSAKEP    50
    KPGQDFFPLT VNYQEKTYAA GKIPGGYFKR EGRPSENETL VSRLIDRPIR 100
    PLFVDGYKND TQVVITVLQH DLENNPDILS MVAASAALTI SGVPFMGPIS 150
    GARVGYIDGE YVLNPNIDEM PESKLDLVVA GTSEAVLMVE SEAQELPEDV 200
    MLGAVMFGHK SFQPVIDAII KLAEVAAKEP RDFQPEDLSE LEAKVLAVVE 250
    NDLREAYKIT EKQARYAAVD AAKAKAKEHF FPEGVEETEM SAEQFATIFK 300
    HLQAKIVRWN ILDTGNRIDG RDLSTVRPIV SEVGILPRTH GSALFTRGET 350
    QAIVVATLGT GEDEQMIDAL TGTYKESFML HYNFPPYSVG ETGRMGSPGR 400
    REIGHGKLAW RAIHPMLPAA EQFPYTIRAV SEITESNGSS SMATVCGTSL 450
    ALMDAGVPIV RPVAGIAMGL IKEGERFAVL SDILGDEDHL GDMDFKVAGT 500
    EFGITSLQMD IKIDGITEEI MKVALEQAKG GRVHILGEMA KAISSSRAEL 550
    GEFAPRIEVM NIPTDKIRDV IGSGGKVIRE IVEKTGAKIN IEDDGTVKIA 600
    SSNGKEIEAA KKWIHSIVAE PEVGEIYEGT VVKTADFGAF VNFFGPRDGL 650
    VHISQLAADR VAKTTDVVKE GQKVWVKLMG FDERGKVRLS MKVVDQETGK 700
    EIVAEKKKEE VDAE 714
    Length:714
    Mass (Da):77,730
    Last modified:February 26, 2008 - v1
    Checksum:i61E6696AD66B29F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000911 Genomic DNA. Translation: ABY39016.1.
    RefSeqiYP_001628587.1. NC_010169.1.

    Genome annotation databases

    EnsemblBacteriaiABY39016; ABY39016; BSUIS_A2006.
    GeneIDi5838194.
    KEGGibmt:BSUIS_A2006.
    PATRICi17871659. VBIBruSui83806_3607.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000911 Genomic DNA. Translation: ABY39016.1 .
    RefSeqi YP_001628587.1. NC_010169.1.

    3D structure databases

    ProteinModelPortali B0CK15.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 470137.BSUIS_A2006.

    Proteomic databases

    PRIDEi B0CK15.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABY39016 ; ABY39016 ; BSUIS_A2006 .
    GeneIDi 5838194.
    KEGGi bmt:BSUIS_A2006.
    PATRICi 17871659. VBIBruSui83806_3607.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218327.
    KOi K00962.
    OMAi HGNFKSN.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci BSUI470137:GJIC-1997-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 23445 / NCTC 10510.

    Entry informationi

    Entry nameiPNP_BRUSI
    AccessioniPrimary (citable) accession number: B0CK15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3