Reviewed, UniProtKB/Swiss-Prot B0CJG9 (AROE_BRUSI)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BSUIS_A1910

Organism

Brucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]

Taxonomic identifier

470137 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000078115
Regions
	Nucleotide binding	134 – 138	5	NADP By similarity
Sites
	Active site	73	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	B0CJG9-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: B0689B2E2675E43B	FASTA	289	30,408
	10 20 30 40 50 60 MDDKSMARGR KAFVTGFPIK HSRSPLIHGF WLKELGIDGS YEAVEVKPED FSSFAASLAA 70 80 90 100 110 120 NGFAGGNVTI PHKEAAYAAA ESLDEAACAI GAVNTLWLEN GRLCGGNTDA YGFAANLDAS 130 140 150 160 170 180 APGWDKADRA LVLGAGGASR AVVHALLSRG VCHVSVVNRT LSRAEELAAH FGARVYAHGW 190 200 210 220 230 240 DEAQALVSNA GLIVNTTALG MSGHGEGQDF PIDLTCAPKE AVATDIVYVP LRTAFLNKAE 250 260 270 280 KAGLKTVDGL GMLLHQAVPG FERWFGQRPQ VTQALREHIL ADMAKAGAL

References

[1]

"Brucella suis ATCC 23445 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000911 Genomic DNA. Translation: ABY38921.1.
RefSeq	YP_001628491.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5838106.
GenomeReviews	Gene locus BSUIS_A1910 in contig CP000911_GR.
KEGG	bmt:BSUIS_A1910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0CJG9. YRAHEVT.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BRUSI

Accession

Primary (citable) accession number: B0CJG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)