B0CJ51 (CYSD_BRUSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	BSUIS_A0193

Organism

Brucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]

Taxonomic identifier

470137 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340188

Sequences

	Sequence		Length	Mass (Da)
	B0CJ51 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: A2EC938F2C141D7A	FASTA	300	34,743
	10 20 30 40 50 60 MKNLTHLQRL EAEAIHVFRE VAATFSNPVM LYSVGKDSSV MLHLAMKAFY PAPPPFPFLH 70 80 90 100 110 120 VDTTWKFREM IEFRDAQARE KGFELLVHVN EQGVRDGIGP FTHGSNVHTH IMKTVGLRQA 130 140 150 160 170 180 LDKYRFDAAF GGARRDEEKS RAKERIFSFR NAQHGWDPKN QRPEMWKIYN TRVSKGESIR 190 200 210 220 230 240 VFPLSNWTEL DIWQYILQEN IPIVPLYFAA RRPVVERDGM LIMVDDDRMK LRPGEQVENR 250 260 270 280 290 300 LVRFRTLGCY PLTGAIPSSA ANLSDIVEEM LIARTSERQG RAIDRDEAGS MEKKKREGYF

References

[1]

"Brucella suis ATCC 23445 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23445 / NCTC 10510.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000911 Genomic DNA. Translation: ABY37295.1.
RefSeq	YP_001626865.1. NC_010169.1.
3D structure databases
ProteinModelPortal	B0CJ51.
SMR	B0CJ51. Positions 4-210.
ModBase	Search...
Protein-protein interaction databases
STRING	B0CJ51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5839212.
GenomeReviews	Gene locus BSUIS_A0193 in contig CP000911_GR.
KEGG	bmt:BSUIS_A0193.
PATRIC	17867852. VBIBruSui83806_1729.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	RVIDHDA.
ProtClustDB	PRK05253.
Enzyme and pathway databases
BioCyc	BSUI470137:BSUIS_A0193-MONOMER.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BRUSI

Accession

Primary (citable) accession number: B0CJ51

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program