Reviewed, UniProtKB/Swiss-Prot B0CGM0 (SYT_BRUSI)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BSUIS_A1115

Organism

Brucella suis (strain ATCC 23445 / NCTC 10510) [Complete proteome] [HAMAP]

Taxonomic identifier

470137 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000077349
Regions
	Region	246 – 548	303	Catalytic HAMAP MF_00184
Sites
	Metal binding	343	1	Zinc; catalytic By similarity
	Metal binding	394	1	Zinc; catalytic By similarity
	Metal binding	525	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0CGM0-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: EA353A28C1C5E3DB	FASTA	658	74,578
	10 20 30 40 50 60 MSNTVSLQFP DGSVREYDAS MTGAALAESI SKSLAKKAVA YAVDGTVRDL SDPLGASGKV 70 80 90 100 110 120 EIITREDPRA LELIRHDTAH VLAEAVQELF PGTQVTIGPV IENGFYYDFA RNEPFTLDDL 130 140 150 160 170 180 PVIEKKMREI IQRNKPFTKE VWSREKAKQV FSDKGESYKV ELVDAIPAGQ DLKIYYQGDW 190 200 210 220 230 240 FDLCRGPHMA STGQIGNSFK LMKVAGAYWR GDANNPMLTR IYGTAFANDN DLQAYLHMLE 250 260 270 280 290 300 EAEKRDHRRL GREMDLFHFQ EEGPGVVFWH AKGWKMFQNL VSYMRRRLDS HGYQEVNAPQ 310 320 330 340 350 360 VLDKSLWETS GHWGWYRDNM FKVTVAGDDT DDDRVFALKP MNCPGHVQIF KHGLKSYRDL 370 380 390 400 410 420 PIKLAEFGNV HRYEPSGALH GLMRVRGFTQ DDAHIFCTEE QMAAECLRIN DLILSVYKDF 430 440 450 460 470 480 GFEEITIKLS TRPEKRVGSD ELWDRAESVM MTVLEQIRQQ SNNIKTGILP GEGAFYGPKF 490 500 510 520 530 540 EYTLKDAIGR EWQCGTTQVD FNLPERFGAF YIGADSEKKQ PVMIHRAICG SMERFLGILI 550 560 570 580 590 600 ENFAGHMPLW FAPVQVVVAT ITSDADEYAK EAAAKLKAAG LQVVTDLRNE KINYKVREHS 610 620 630 640 650 LQKVPVILVC GKREAEEKTV NMRRLGSRDQ ESMTLDEAIA RLCEEATPPD LLRLKNAG

References

[1]

"Brucella suis ATCC 23445 whole genome shotgun sequencing project." Setubal J.C., Bowns C., Boyle S., Crasta O.R., Czar M.J., Dharmanolla C., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Mohapatra S., Nagrani S., Purkayastha A., Rajasimha H.K., Shallom J.M., Shallom S., Shukla M., Snyder E.E. , Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Bruce D., Detter C., Munk C., Brettin T.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000911 Genomic DNA. Translation: ABY38171.1.
RefSeq	YP_001627741.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5838430.
GenomeReviews	Gene locus BSUIS_A1115 in contig CP000911_GR.
KEGG	bmt:BSUIS_A1115.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QDEAPGM.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BRUSI

Accession

Primary (citable) accession number: B0CGM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)