Reviewed, UniProtKB/Swiss-Prot B0CFX4 (SYA_ACAM1)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AM1_4444

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 873	873	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347466

Sequences

	Sequence		Length	Mass (Da)
	B0CFX4-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 7AAF9E6767E519C2	FASTA	873	95,642
	10 20 30 40 50 60 MVASLSGAQI RQTFLDFYAA KGHKILPSAS LVPEDPTVLL TIAGMLPFKP IFLGQREAEV 70 80 90 100 110 120 PRATTSQKCI RTNDIENVGQ TARHHTYFEM LGNFSFGDYF KEQAIAWAWE LSTEVYKLPP 130 140 150 160 170 180 ERIVPSVFEE DDEAFAIWRD KIGIPEHRIQ RMGAEDNFWA SGPTGPCGPC SELYYDFYPE 190 200 210 220 230 240 KGDDKIDLED DTRFIEYYNL VFMEYNRDSD GKLAPLKNKN IDTGLGLERM AQILQGVPNN 250 260 270 280 290 300 YETDLIFPII ETAAKIAGIK YKKSKAKTKT SLKVVGDHVR SVVQMIADGI TASNVGRGYV 310 320 330 340 350 360 LRRLIRRVVR HGQLIGIDGA FITQVAETAI ASLEVAYPEV REREKIIKTE LEREEAQFLK 370 380 390 400 410 420 TLNRGEKLLG EIMAKKPKQI SGKDAFDLYD TYGFPLELTQ EIAAEQGLTV DEDGFAKAMK 430 440 450 460 470 480 EQQDRGRSAH KTIDLTVQSA LEQLAATVHP TDFLGYTDFS AKAKVAALLV KGETVDQASA 490 500 510 520 530 540 GSEVQIALDQ TPFYAESGGQ IGDRGYLNGK DVVVRIEDVQ KESDIFIHYG RIERGTLEVG 550 560 570 580 590 600 DKLTAQIDLA CRRQVQAHHT ATHLLQAALK NIVDESIGQA GSLVAFDRLR FDFNYNQAVT 610 620 630 640 650 660 PEQIQEIETQ INTWIAEAHT TETEVMPIAE AKAKGAVAMF GEKYGAEVRV MDVPGVSMEL 670 680 690 700 710 720 CGGTHVKNTS EIGLFKIVTE AGVASGVRRI EAIAGPAVLE YLNVRDAVVR DLSDRFKAKP 730 740 750 760 770 780 EELPERITTL QADLKTAQKQ LDTLKAQLAL VKSEQLLDQA EPAGEVKVLV SQLEGVDSES 790 800 810 820 830 840 LKTAAGRLLQ KLGEGAVVLG SVPAEGKVSL VAAFSPKVIE QGLQAGKFVG AIAKQCGGGG 850 860 870 GGRPNLAQAG GRDPSKLADA LDDAQKQLLA QLK

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW29421.1.
RefSeq	YP_001518738.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5683246.
GenomeReviews	Gene locus AM1_4444 in contig CP000828_GR.
KEGG	amr:AM1_4444.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0CFX4. GEFVIKL.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACAM1

Accession

Primary (citable) accession number: B0CFX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)