B0CFX4 (SYA_ACAM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AM1_4444

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Acaryochloris

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 873	873	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347466
Sites
	Metal binding	559	1	Zinc Potential
	Metal binding	563	1	Zinc Potential
	Metal binding	661	1	Zinc Potential
	Metal binding	665	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	B0CFX4 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 7AAF9E6767E519C2	FASTA	873	95,642
	10 20 30 40 50 60 MVASLSGAQI RQTFLDFYAA KGHKILPSAS LVPEDPTVLL TIAGMLPFKP IFLGQREAEV 70 80 90 100 110 120 PRATTSQKCI RTNDIENVGQ TARHHTYFEM LGNFSFGDYF KEQAIAWAWE LSTEVYKLPP 130 140 150 160 170 180 ERIVPSVFEE DDEAFAIWRD KIGIPEHRIQ RMGAEDNFWA SGPTGPCGPC SELYYDFYPE 190 200 210 220 230 240 KGDDKIDLED DTRFIEYYNL VFMEYNRDSD GKLAPLKNKN IDTGLGLERM AQILQGVPNN 250 260 270 280 290 300 YETDLIFPII ETAAKIAGIK YKKSKAKTKT SLKVVGDHVR SVVQMIADGI TASNVGRGYV 310 320 330 340 350 360 LRRLIRRVVR HGQLIGIDGA FITQVAETAI ASLEVAYPEV REREKIIKTE LEREEAQFLK 370 380 390 400 410 420 TLNRGEKLLG EIMAKKPKQI SGKDAFDLYD TYGFPLELTQ EIAAEQGLTV DEDGFAKAMK 430 440 450 460 470 480 EQQDRGRSAH KTIDLTVQSA LEQLAATVHP TDFLGYTDFS AKAKVAALLV KGETVDQASA 490 500 510 520 530 540 GSEVQIALDQ TPFYAESGGQ IGDRGYLNGK DVVVRIEDVQ KESDIFIHYG RIERGTLEVG 550 560 570 580 590 600 DKLTAQIDLA CRRQVQAHHT ATHLLQAALK NIVDESIGQA GSLVAFDRLR FDFNYNQAVT 610 620 630 640 650 660 PEQIQEIETQ INTWIAEAHT TETEVMPIAE AKAKGAVAMF GEKYGAEVRV MDVPGVSMEL 670 680 690 700 710 720 CGGTHVKNTS EIGLFKIVTE AGVASGVRRI EAIAGPAVLE YLNVRDAVVR DLSDRFKAKP 730 740 750 760 770 780 EELPERITTL QADLKTAQKQ LDTLKAQLAL VKSEQLLDQA EPAGEVKVLV SQLEGVDSES 790 800 810 820 830 840 LKTAAGRLLQ KLGEGAVVLG SVPAEGKVSL VAAFSPKVIE QGLQAGKFVG AIAKQCGGGG 850 860 870 GGRPNLAQAG GRDPSKLADA LDDAQKQLLA QLK

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBIC 11017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000828 Genomic DNA. Translation: ABW29421.1.
RefSeq	YP_001518738.1. NC_009925.1.
3D structure databases
ProteinModelPortal	B0CFX4.
ModBase	Search...
Protein-protein interaction databases
STRING	B0CFX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5683246.
GenomeReviews	Gene locus AM1_4444 in contig CP000828_GR.
KEGG	amr:AM1_4444.
PATRIC	20622972. VBIAcaMar40141_4107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	GESKTDQ.
PhylomeDB	B0CFX4.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	AMAR329726:AM1_4444-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACAM1

Accession

Primary (citable) accession number: B0CFX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program