Reviewed, UniProtKB/Swiss-Prot B0CFL0 (PROA_ACAM1)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	AM1_2014

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000080474

Sequences

	Sequence		Length	Mass (Da)
	B0CFL0-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: CCD327A05288B456	FASTA	431	45,666
	10 20 30 40 50 60 MTVLTNSLTE VAQQTRQAAI QLAGASTETK NQALEAVAQA LEAATPEILE ANVQDCQQAK 70 80 90 100 110 120 AAGIANALYA RLKLDATKLK GAIAGVRSVA TLEDPVGTVQ LKRELDTDLV LSRVSCPLGV 130 140 150 160 170 180 LGVIFEARPD AVMQIAALAM KSGNGVLLKG GQEALHSCQA LVKAIHQGLS NTAVSPNVVA 190 200 210 220 230 240 LLTSREETLE LLNLDQDVNL IIPRGSNDFV RFVQENTRIP VLGHAEGICH LYVDQAAKLD 250 260 270 280 290 300 QAVEIAVDSK IQYPAACNAV ETILVHDAIA PQYLPKLTAA LQDNHVKVLG DAKTQAIVDV 310 320 330 340 350 360 GPATETDWAT EYGDLIVSIK VVASLSDAIA HINQYGSGHT DAIATEDDQA AAVFMNQVDA 370 380 390 400 410 420 AGVFHNCSTR FADGFRYGFG AEVGISTQRM PPRGPVGLEG LVTYKYQLKG QGQIVATYAG 430 SDARPFTHKD L

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW27029.1.
RefSeq	YP_001516343.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5680828.
GenomeReviews	Gene locus AM1_2014 in contig CP000828_GR.
KEGG	amr:AM1_2014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0CFL0. QYPAACN.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACAM1

Accession

Primary (citable) accession number: B0CFL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)