Reviewed, UniProtKB/Swiss-Prot B0CF78 (PYRC_ACAM1)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	AM1_5644

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6. HAMAP MF_00219
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Dihydroorotase HAMAP MF_00219		PRO_1000078095
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	247	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0CF78-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: F512F4502DA91F41	FASTA	345	38,498
	10 20 30 40 50 60 MQKLTLTRPD DWHLHLRDGD ALKAVLPHSA RQFARAIVMP NLKPPVRTVA DAADYRNRIL 70 80 90 100 110 120 AALPVGQTFE PLMTLYLTDN TSPEEISAAK ASKFVKAVKY YPAGATTNSD FGVTDIRKCD 130 140 150 160 170 180 RIFAAMEQED IPLLLHGEVT DTNIDVFDRE KVFIETHLIP LVSRFPKLRV VLEHITTSEA 190 200 210 220 230 240 VTFVLNTNEN IGATITPQHL LFSRNAIFKG GIRPHYYCLP ILKREKHRQA LLQAATSGNP 250 260 270 280 290 300 KFFLGTDSAP HARNSKEQSC GCAGCYSALH AMELYAEAFE SANALDKLEA FASFYGPDFY 310 320 330 340 QLPRNTKTIT LAKQTWQIPD EVPFPGTGLV PLRAGEEITW KIMDG

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW30594.1.
RefSeq	YP_001519913.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5684434.
GenomeReviews	Gene locus AM1_5644 in contig CP000828_GR.
KEGG	amr:AM1_5644.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0CF78. IMPNLVP.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ACAM1

Accession

Primary (citable) accession number: B0CF78

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)