Reviewed, UniProtKB/Swiss-Prot B0CF01 (SYD_ACAM1)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	AM1_4421

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000074689

Sequences

	Sequence		Length	Mass (Da)
	B0CF01-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 5EBCB95AA2AF7EAA	FASTA	595	67,537
	10 20 30 40 50 60 MRTHYCSELR NDHVGERVTL CGWVDRRRDH GGVIFLDVRD RTGLIQIVSD PERTPEAYPQ 70 80 90 100 110 120 ADALRNEYVV RIHGEVSRRP DDSLNPRLPT GEVEIYADQI ELLNAVHQQL PFQVSTADVE 130 140 150 160 170 180 SVKEELRLKY RYLDLRRDRM HQNIMMRHRL IQSIRRYLED QQNFVDIETP VLTRSTPEGA 190 200 210 220 230 240 RDYLVPSRAN PGEWFALPQS PQIFKQLLMV SGFDRYYQIA RCFRDEDLRA DRQPEFTQLD 250 260 270 280 290 300 MEMSFMSQEE IIQLNEDLMR HVFKTLKDID LPKTFPQMTY AEAMERFGTD RPDTRFDLEL 310 320 330 340 350 360 VNVSDLLEDS GFKVFSGAIK KGGQVKVLPI PDGNAKISNV RIKPGGDLFS EASAGGAKGL 370 380 390 400 410 420 AYIRVKDDGA IDTIGAIKDN LSPEQTAELL ERTGAKPGHL LLFGAGPTEI VNASLSRLRL 430 440 450 460 470 480 ALGAEMKLID PDQIDLLWIT EFPMFEWNAD EKRLEALHHP FTAPYPEDEA DLKTARAQAY 490 500 510 520 530 540 DLVYNGLEIG GGSLRIYQAD LQRRVFDAIG LSEEEAQDKF GFLLNAFNFG APPHGGIAYG 550 560 570 580 590 IDRLAMLLSG EESIRDTIAF PKTQQARCLL TQAPSSVDQK QLKELQIKST AEPKN

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW29398.1.
RefSeq	YP_001518715.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5683223.
GenomeReviews	Gene locus AM1_4421 in contig CP000828_GR.
KEGG	amr:AM1_4421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VDRRRDH.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACAM1

Accession

Primary (citable) accession number: B0CF01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)