Reviewed, UniProtKB/Swiss-Prot B0CEN4 (ILVD_ACAM1)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	AM1_3143

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000073964
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B0CEN4-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 6AD0910743DE0659	FASTA	561	58,905
	10 20 30 40 50 60 MSDNRNSQVV TQGVQRAPNR AMLRAVGFGD DDFTKPIVGL ANGFSTITPC NMGIDSLATR 70 80 90 100 110 120 AEASIRTAGA MPQKFGTITI SDGISMGTEG MKYSLVSREV IADSIETACM GQSMDGVLAI 130 140 150 160 170 180 GGCDKNMPGA MLAMARMNIP AIFVYGGTIK PGHLNGEDLT VVSAFEAVGQ HSAGRISEAE 190 200 210 220 230 240 LTAVEKHACP GAGSCGGMYT ANTMSSAFEA MGMSLMYSST MAAEDEEKAV SAEQSAAVLV 250 260 270 280 290 300 EAIHKQILPR DILTRKAFEN AIAVIMAVGG STNAVLHLLA ISRAAGDSLT LDDFETIRAQ 310 320 330 340 350 360 VPVICDLKPS GRYVATDLHK AGGIPLVMKM LLEHGLLHGD ALTITGKTIA EQLADVPSEP 370 380 390 400 410 420 SPDQDVIRPW DNPMYKQGHL AILRGNLATE GAVAKITGIK NPQITGPARV FESEEACLEA 430 440 450 460 470 480 ILAGKIQPND VIVVRYEGPK GGPGMREMLA PTSAIIGAGL GDSVGLITDG RFSGGTYGMV 490 500 510 520 530 540 VGHVAPEAAV GGTIALVQEG DQITIDAHAR KLELHVSDQE LKERKEKWEQ PKPLYNKGVL 550 560 AKYAKLVSSS SVGAVTDLDL F

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW28139.1.
RefSeq	YP_001517455.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5681951.
GenomeReviews	Gene locus AM1_3143 in contig CP000828_GR.
KEGG	amr:AM1_3143.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QVMKILL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ACAM1

Accession

Primary (citable) accession number: B0CEN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)