B0CCF6 (AMPA_ACAM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	AM1_2989

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Acaryochloris

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000077272
Sites
	Active site	274	1	Potential
	Active site	349	1	Potential
	Metal binding	262	1	Manganese 2 By similarity
	Metal binding	267	1	Manganese 1 By similarity
	Metal binding	267	1	Manganese 2 By similarity
	Metal binding	286	1	Manganese 2 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0CCF6 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: AAFF301F74DD17C8	FASTA	492	51,870
	10 20 30 40 50 60 MKIQAIETVA ADWSGDYLGI GFFEDSAAID AGLTQLDQSL QDILQDLVSE TEFQGKVGEQ 70 80 90 100 110 120 PWTRIAGPGS IRKVMLIGLG SPDAFTLDSL RQAAAAFAKA AQKQKAASAG LQLPSWDDDA 130 140 150 160 170 180 AASTQAMAEG IELALHQEIR FKSDPDAKKP GEFPQEVHLL GLANQAAAIE KAQQICAGVI 190 200 210 220 230 240 LTRELVAAPA NVVTPSALAE TAAQIAEDHG LTLEVLEREE CEAQGMGAFL GVSLASELPP 250 260 270 280 290 300 KFIHLTYKPS GTPRRKLAIV GKGVTFDSGG LNLKVGGSGI ETMKMDMAGS GATLGAAKAI 310 320 330 340 350 360 GQLKPDVEVH FIVAAAENMI SGHALHPGDI LTASNGKTIE INNTDAEGRL TLADALVFAE 370 380 390 400 410 420 KQGVDAIVDL ATLTGACIVA LGNDIAGMWT PEDSLAEELS QASEQAGEKF WRMPLEEKYF 430 440 450 460 470 480 EGLKSPIADM KNTGPRPGGS ITAALFLKQY IENTPWAHLD VAGPVWTEKE NGYLNVGATG 490 FAVRTLVNWV MG

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBIC 11017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000828 Genomic DNA. Translation: ABW27985.1.
RefSeq	YP_001517301.1. NC_009925.1.
3D structure databases
ProteinModelPortal	B0CCF6.
ModBase	Search...
Protein-protein interaction databases
STRING	B0CCF6.
Protein family/group databases
MEROPS	M17.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5681797.
GenomeReviews	Gene locus AM1_2989 in contig CP000828_GR.
KEGG	amr:AM1_2989.
PATRIC	20620258. VBIAcaMar40141_2757.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	KLKYAED.
PhylomeDB	B0CCF6.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	AMAR329726:AM1_2989-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ACAM1

Accession

Primary (citable) accession number: B0CCF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program