Reviewed, UniProtKB/Swiss-Prot B0CBU2 (PURA_ACAM1)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	AM1_5386

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity.
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000073935
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	139	1	By similarity
	Active site	146	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0CBU2-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 3A02F7D02BA0A205	FASTA	446	49,192
	10 20 30 40 50 60 MANVVVIGAQ WGDEGKGKIT DLLSRSADVV VRYQGGVNAG HTVVVNDQTF KLHLIPSGIL 70 80 90 100 110 120 YPNTDCIIGS GTVIDPKVLI EELDMLDKLG VSTSHLFISE SAHVTMPYHR MIDQASEERR 130 140 150 160 170 180 GDHKIGTTGR GIGPTYADKS ERTGIRILDL MDPEGMKKQL RWTVNYKNVI LEKLYNLPHL 190 200 210 220 230 240 DPEEVIAEYQ VYADRLRPFV ADCSLKIYDA YQQHRNILFE GAQGTLLDLD HGTYPYVTSS 250 260 270 280 290 300 NPVAGGACVG TGVGPTMIDR VIGVAKAYTT RVGEGPFPTE LHDEMGVALC ERGAEFGTTT 310 320 330 340 350 360 GRRRRCGWFD AVIGRYAVRI NGLDCLAVTK LDILDEVDEI KVCVAYEIDG HESKDFPTNA 370 380 390 400 410 420 RQFSRCNPVY KTLPGWKQST IHCRTLEDLP PKALDYLKFL ASIVEVPIAI VSLGAERDET 430 440 IIVEDPIHGP KRALLYSNGE SQAMSA

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW30342.1.
RefSeq	YP_001519661.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5684178.
GenomeReviews	Gene locus AM1_5386 in contig CP000828_GR.
KEGG	amr:AM1_5386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YVLGIIK.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ACAM1

Accession

Primary (citable) accession number: B0CBU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)