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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Acaryochloris marina (strain MBIC 11017)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi146Zinc 1UniRule annotation1
Metal bindingi149Zinc 1UniRule annotation1
Metal bindingi163Zinc 2UniRule annotation1
Metal bindingi166Zinc 2UniRule annotation1
Metal bindingi189Zinc 2UniRule annotation1
Metal bindingi192Zinc 2UniRule annotation1
Metal bindingi203Zinc 1UniRule annotation1
Metal bindingi206Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 215CR-typeUniRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:AM1_0423
OrganismiAcaryochloris marina (strain MBIC 11017)
Taxonomic identifieri329726 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesAcaryochloridaceaeAcaryochloris
Proteomesi
  • UP000000268 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000851311 – 374Chaperone protein DnaJAdd BLAST374

Proteomic databases

PRIDEiB0CAZ0.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi329726.AM1_0423.

Structurei

3D structure databases

ProteinModelPortaliB0CAZ0.
SMRiB0CAZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 68JUniRule annotationAdd BLAST65
Repeati146 – 153CXXCXGXG motif8
Repeati163 – 170CXXCXGXG motif8
Repeati189 – 196CXXCXGXG motif8
Repeati203 – 210CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 116Gly-richAdd BLAST42

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 215CR-typeUniRule annotationAdd BLAST83

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.
OrthoDBiPOG091H00PT.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

B0CAZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDYYDILG VSRSADPDEL KRSYRRLARK YHPDVNKEPG AEDKFKEINK
60 70 80 90 100
AYETLSDPQM RGRYDQFGEA GVSSAAGAGY QDFGDFGGFA DIFETFFSGF
110 120 130 140 150
GGSPQSGRRR SGPARGEDLR FDLKLKFREA VFGGEQQIRI SHLESCKTCE
160 170 180 190 200
GTGAKPGTRP QTCSTCQGSG QVRRMTRTPL GNFTQVSVCP TCNGQGQVVT
210 220 230 240 250
EKCDSCGGRG QNQVSKKLQI KIPAGVDTGT RLRVSNEGDA GQKGGPPGDL
260 270 280 290 300
YVYLFVQEDS EFRRDGTNVL SEFKISYLQA ILGAQLPVKT VDGEETITIP
310 320 330 340 350
AGTQPGTVLT LENHGVPRLG NPVSRGDHLI TVIIDIPNRI STEERELLVQ
360 370
LASIRAEQTG KGGIEGFLGG IFGR
Length:374
Mass (Da):40,631
Last modified:February 26, 2008 - v1
Checksum:i4DF3713716834806
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000828 Genomic DNA. Translation: ABW25480.1.
RefSeqiWP_012161087.1. NC_009925.1.

Genome annotation databases

EnsemblBacteriaiABW25480; ABW25480; AM1_0423.
KEGGiamr:AM1_0423.

Similar proteinsi

Entry informationi

Entry nameiDNAJ_ACAM1
AccessioniPrimary (citable) accession number: B0CAZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 26, 2008
Last modified: June 7, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families