Reviewed, UniProtKB/Swiss-Prot B0CA74 (PYRD_ACAM1)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	AM1_1640

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Dihydroorotate dehydrogenase		PRO_1000078153
Sites
	Active site	195	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0CA74-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 32BF33B7ACCA9F72	FASTA	374	40,679
	10 20 30 40 50 60 MDLYQQGLQP LLFSALKADP ETVHRQLMRT CAWLDRNVDQ GLAQGLQRRL ASSLQVEDPR 70 80 90 100 110 120 LSQSLWGLSF ANPIGLAAGF DKDGVATNIW PRFGFGFAEV GTVTFHAQPG NPQPRLFRLP 130 140 150 160 170 180 EDQAALNRMG FNNQGAAAMA KVIAASLDRQ ARSYPLGINL GKSKVTPLEQ AVADYVGSFQ 190 200 210 220 230 240 LLKTYGDYFV VNVSSPNTPG LRSLQAVEQL APILAGLQAE NTEGKPLLVK IAPDLEWKDI 250 260 270 280 290 300 AAIVDLAQVH QLAGIIATNT TIRRDLKTER IAATGNVPSE EAGGISGAPV RSRSTDVIRF 310 320 330 340 350 360 IHKQTQGQLP IIGVGGIFTA EDAWEKLCAG ASLLQVYTGW VYEGPWMVRR ILEGLLVKMQ 370 EEGIQQLSEI VGQK

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW26661.1.
RefSeq	YP_001515975.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5680456.
GenomeReviews	Gene locus AM1_1640 in contig CP000828_GR.
KEGG	amr:AM1_1640.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. DHO_DHase_1_2. IPR005719. DHO_DHase_2. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ACAM1

Accession

Primary (citable) accession number: B0CA74

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 25, 2008
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)