Reviewed, UniProtKB/Swiss-Prot B0C872 (SYI_ACAM1)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	AM1_3907

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	971 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 971	971	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_1000088541
Regions
	Motif	60 – 70	11	"HIGH" region HAMAP MF_02002
	Motif	604 – 608	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	922	1	Zinc By similarity
	Metal binding	925	1	Zinc By similarity
	Metal binding	942	1	Zinc By similarity
	Metal binding	945	1	Zinc By similarity
	Binding site	563	1	Aminoacyl-adenylate By similarity
	Binding site	607	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0C872-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 206CB68F80AB54D3	FASTA	971	109,792
	10 20 30 40 50 60 MTEPGRYKKT VNLPKTKFDM RANAVKREPE LQKFWADHQI YENLSQNNPG DVFVLHDGPP 70 80 90 100 110 120 YANGDLHIGH ALNKILKDTI NKFQLLQGRK VRYVPGWDCH GLPIELKVLQ NIQPENRAKL 130 140 150 160 170 180 TPLKLRWKAR DFALKTVEKQ SKSFQRYGVW GNWENPYLTL KPEYEAAQIG VFGKMALKGY 190 200 210 220 230 240 IYRGFKPVYW SPSSQTALAE AELEYPEGHT SRSIYVTFKV TGLSETAQPL LDEYLPTLKV 250 260 270 280 290 300 AIWTTTPWTI PGNLAVSLNP DLTYAVVKAG EDYLIVAEDL VETLTETLES SFKVIKTLPG 310 320 330 340 350 360 KALENSTYQH PLFEREGPLV LGDYVTTESG TGLVHTAPGH GQEDYQVGQQ YGLAMLSPVD 370 380 390 400 410 420 GDGTFTDEAG PFAGLNVLNG GNEAVIEALQ SAGALLKEES YAHKYPYDWR TKKPVILRAT 430 440 450 460 470 480 EQWFASVDGF REAVLDAIAT VNWIPAQGEN RITSMVSERS DWCISRQRNW GVPIPVFYND 490 500 510 520 530 540 ATGEPLLNEA TVNHIQGIVA EKGSDAWWEL DNDELLPEPY RSDGNTYRKG TDTMDVWFDS 550 560 570 580 590 600 GSSWAAVCDQ REPLKYPAEM YLEGSDQHRG WFQSSILTSV ATNGHAPYKT VLTHGFVLDE 610 620 630 640 650 660 QGRKMSKSIG NVVDPAIVIA GGKNQKQDPP YGADVLRLWV SSVDYASDVP LGKNILKQMA 670 680 690 700 710 720 DVYRKIRNTS RFLLGNLHDF DPAQDAVAYE DLPQLDRYML HRITEVFTDV TEAFESFQFF 730 740 750 760 770 780 RFFQTVQNFC VVDLSNFYLD IAKDRLYISE PNAQRRRSCQ TVLAIALENL ARAIAPVLSH 790 800 810 820 830 840 MAEDIWQSLP YETEHQSVFA SGWMRLEDQW HNPDLASHWI VLREIRQEVN KVLEQARTEK 850 860 870 880 890 900 EIGSSLEAKV LLYVSDTDLR QQLDAMNPST GGGSNNVDEL RYLFLASQVE LLETPKNLDR 910 920 930 940 950 960 LMYQFQSETL GVGVVTADGK KCDRCWNYST YVGRSKQHPL LCDRCEPIIE NLVTQGQISL 970 TEEGRYQPNT K

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW28892.1.
RefSeq	YP_001518209.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5682711.
GenomeReviews	Gene locus AM1_3907 in contig CP000828_GR.
KEGG	amr:AM1_3907.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FPMRGNL.
Family and domain databases
HAMAP	MF_02002. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_ACAM1

Accession

Primary (citable) accession number: B0C872

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)