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B0C872

- SYI_ACAM1

UniProt

B0C872 - SYI_ACAM1

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Acaryochloris marina (strain MBIC 11017)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei607 – 6071ATPUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi945 – 9451ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAMAR329726:GCZJ-3894-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:AM1_3907
OrganismiAcaryochloris marina (strain MBIC 11017)
Taxonomic identifieri329726 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesAcaryochloris
ProteomesiUP000000268: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971Isoleucine--tRNA ligasePRO_1000088541Add
BLAST

Proteomic databases

PRIDEiB0C872.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi329726.AM1_3907.

Structurei

3D structure databases

ProteinModelPortaliB0C872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi604 – 6085"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0C872-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEPGRYKKT VNLPKTKFDM RANAVKREPE LQKFWADHQI YENLSQNNPG
60 70 80 90 100
DVFVLHDGPP YANGDLHIGH ALNKILKDTI NKFQLLQGRK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ NIQPENRAKL TPLKLRWKAR DFALKTVEKQ SKSFQRYGVW
160 170 180 190 200
GNWENPYLTL KPEYEAAQIG VFGKMALKGY IYRGFKPVYW SPSSQTALAE
210 220 230 240 250
AELEYPEGHT SRSIYVTFKV TGLSETAQPL LDEYLPTLKV AIWTTTPWTI
260 270 280 290 300
PGNLAVSLNP DLTYAVVKAG EDYLIVAEDL VETLTETLES SFKVIKTLPG
310 320 330 340 350
KALENSTYQH PLFEREGPLV LGDYVTTESG TGLVHTAPGH GQEDYQVGQQ
360 370 380 390 400
YGLAMLSPVD GDGTFTDEAG PFAGLNVLNG GNEAVIEALQ SAGALLKEES
410 420 430 440 450
YAHKYPYDWR TKKPVILRAT EQWFASVDGF REAVLDAIAT VNWIPAQGEN
460 470 480 490 500
RITSMVSERS DWCISRQRNW GVPIPVFYND ATGEPLLNEA TVNHIQGIVA
510 520 530 540 550
EKGSDAWWEL DNDELLPEPY RSDGNTYRKG TDTMDVWFDS GSSWAAVCDQ
560 570 580 590 600
REPLKYPAEM YLEGSDQHRG WFQSSILTSV ATNGHAPYKT VLTHGFVLDE
610 620 630 640 650
QGRKMSKSIG NVVDPAIVIA GGKNQKQDPP YGADVLRLWV SSVDYASDVP
660 670 680 690 700
LGKNILKQMA DVYRKIRNTS RFLLGNLHDF DPAQDAVAYE DLPQLDRYML
710 720 730 740 750
HRITEVFTDV TEAFESFQFF RFFQTVQNFC VVDLSNFYLD IAKDRLYISE
760 770 780 790 800
PNAQRRRSCQ TVLAIALENL ARAIAPVLSH MAEDIWQSLP YETEHQSVFA
810 820 830 840 850
SGWMRLEDQW HNPDLASHWI VLREIRQEVN KVLEQARTEK EIGSSLEAKV
860 870 880 890 900
LLYVSDTDLR QQLDAMNPST GGGSNNVDEL RYLFLASQVE LLETPKNLDR
910 920 930 940 950
LMYQFQSETL GVGVVTADGK KCDRCWNYST YVGRSKQHPL LCDRCEPIIE
960 970
NLVTQGQISL TEEGRYQPNT K
Length:971
Mass (Da):109,792
Last modified:February 26, 2008 - v1
Checksum:i206CB68F80AB54D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000828 Genomic DNA. Translation: ABW28892.1.
RefSeqiWP_012164256.1. NC_009925.1.
YP_001518209.1. NC_009925.1.

Genome annotation databases

EnsemblBacteriaiABW28892; ABW28892; AM1_3907.
GeneIDi5682711.
KEGGiamr:AM1_3907.
PATRICi20621992. VBIAcaMar40141_3622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000828 Genomic DNA. Translation: ABW28892.1 .
RefSeqi WP_012164256.1. NC_009925.1.
YP_001518209.1. NC_009925.1.

3D structure databases

ProteinModelPortali B0C872.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 329726.AM1_3907.

Proteomic databases

PRIDEi B0C872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABW28892 ; ABW28892 ; AM1_3907 .
GeneIDi 5682711.
KEGGi amr:AM1_3907.
PATRICi 20621992. VBIAcaMar40141_3622.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AMAR329726:GCZJ-3894-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBIC 11017.

Entry informationi

Entry nameiSYI_ACAM1
AccessioniPrimary (citable) accession number: B0C872
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 26, 2008
Last modified: October 29, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3