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B0C872

- SYI_ACAM1

UniProt

B0C872 - SYI_ACAM1

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Protein
Isoleucine--tRNA ligase
Gene
ileS, AM1_3907
Organism
Acaryochloris marina (strain MBIC 11017)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylate By similarity
Binding sitei607 – 6071ATP By similarity
Metal bindingi922 – 9221Zinc By similarity
Metal bindingi925 – 9251Zinc By similarity
Metal bindingi942 – 9421Zinc By similarity
Metal bindingi945 – 9451Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAMAR329726:GCZJ-3894-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:AM1_3907
OrganismiAcaryochloris marina (strain MBIC 11017)
Taxonomic identifieri329726 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesAcaryochloris
ProteomesiUP000000268: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971Isoleucine--tRNA ligaseUniRule annotation
PRO_1000088541Add
BLAST

Proteomic databases

PRIDEiB0C872.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi329726.AM1_3907.

Structurei

3D structure databases

ProteinModelPortaliB0C872.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi604 – 6085"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0C872-1 [UniParc]FASTAAdd to Basket

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MTEPGRYKKT VNLPKTKFDM RANAVKREPE LQKFWADHQI YENLSQNNPG    50
DVFVLHDGPP YANGDLHIGH ALNKILKDTI NKFQLLQGRK VRYVPGWDCH 100
GLPIELKVLQ NIQPENRAKL TPLKLRWKAR DFALKTVEKQ SKSFQRYGVW 150
GNWENPYLTL KPEYEAAQIG VFGKMALKGY IYRGFKPVYW SPSSQTALAE 200
AELEYPEGHT SRSIYVTFKV TGLSETAQPL LDEYLPTLKV AIWTTTPWTI 250
PGNLAVSLNP DLTYAVVKAG EDYLIVAEDL VETLTETLES SFKVIKTLPG 300
KALENSTYQH PLFEREGPLV LGDYVTTESG TGLVHTAPGH GQEDYQVGQQ 350
YGLAMLSPVD GDGTFTDEAG PFAGLNVLNG GNEAVIEALQ SAGALLKEES 400
YAHKYPYDWR TKKPVILRAT EQWFASVDGF REAVLDAIAT VNWIPAQGEN 450
RITSMVSERS DWCISRQRNW GVPIPVFYND ATGEPLLNEA TVNHIQGIVA 500
EKGSDAWWEL DNDELLPEPY RSDGNTYRKG TDTMDVWFDS GSSWAAVCDQ 550
REPLKYPAEM YLEGSDQHRG WFQSSILTSV ATNGHAPYKT VLTHGFVLDE 600
QGRKMSKSIG NVVDPAIVIA GGKNQKQDPP YGADVLRLWV SSVDYASDVP 650
LGKNILKQMA DVYRKIRNTS RFLLGNLHDF DPAQDAVAYE DLPQLDRYML 700
HRITEVFTDV TEAFESFQFF RFFQTVQNFC VVDLSNFYLD IAKDRLYISE 750
PNAQRRRSCQ TVLAIALENL ARAIAPVLSH MAEDIWQSLP YETEHQSVFA 800
SGWMRLEDQW HNPDLASHWI VLREIRQEVN KVLEQARTEK EIGSSLEAKV 850
LLYVSDTDLR QQLDAMNPST GGGSNNVDEL RYLFLASQVE LLETPKNLDR 900
LMYQFQSETL GVGVVTADGK KCDRCWNYST YVGRSKQHPL LCDRCEPIIE 950
NLVTQGQISL TEEGRYQPNT K 971
Length:971
Mass (Da):109,792
Last modified:February 26, 2008 - v1
Checksum:i206CB68F80AB54D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000828 Genomic DNA. Translation: ABW28892.1.
RefSeqiYP_001518209.1. NC_009925.1.

Genome annotation databases

EnsemblBacteriaiABW28892; ABW28892; AM1_3907.
GeneIDi5682711.
KEGGiamr:AM1_3907.
PATRICi20621992. VBIAcaMar40141_3622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000828 Genomic DNA. Translation: ABW28892.1 .
RefSeqi YP_001518209.1. NC_009925.1.

3D structure databases

ProteinModelPortali B0C872.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 329726.AM1_3907.

Proteomic databases

PRIDEi B0C872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABW28892 ; ABW28892 ; AM1_3907 .
GeneIDi 5682711.
KEGGi amr:AM1_3907.
PATRICi 20621992. VBIAcaMar40141_3622.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AMAR329726:GCZJ-3894-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MBIC 11017.

Entry informationi

Entry nameiSYI_ACAM1
AccessioniPrimary (citable) accession number: B0C872
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 26, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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