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B0C872

- SYI_ACAM1

UniProt

B0C872 - SYI_ACAM1

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Acaryochloris marina (strain MBIC 11017)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
    Binding sitei607 – 6071ATPUniRule annotation
    Metal bindingi922 – 9221ZincUniRule annotation
    Metal bindingi925 – 9251ZincUniRule annotation
    Metal bindingi942 – 9421ZincUniRule annotation
    Metal bindingi945 – 9451ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAMAR329726:GCZJ-3894-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:AM1_3907
    OrganismiAcaryochloris marina (strain MBIC 11017)
    Taxonomic identifieri329726 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesAcaryochloris
    ProteomesiUP000000268: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 971971Isoleucine--tRNA ligasePRO_1000088541Add
    BLAST

    Proteomic databases

    PRIDEiB0C872.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi329726.AM1_3907.

    Structurei

    3D structure databases

    ProteinModelPortaliB0C872.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0C872-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEPGRYKKT VNLPKTKFDM RANAVKREPE LQKFWADHQI YENLSQNNPG    50
    DVFVLHDGPP YANGDLHIGH ALNKILKDTI NKFQLLQGRK VRYVPGWDCH 100
    GLPIELKVLQ NIQPENRAKL TPLKLRWKAR DFALKTVEKQ SKSFQRYGVW 150
    GNWENPYLTL KPEYEAAQIG VFGKMALKGY IYRGFKPVYW SPSSQTALAE 200
    AELEYPEGHT SRSIYVTFKV TGLSETAQPL LDEYLPTLKV AIWTTTPWTI 250
    PGNLAVSLNP DLTYAVVKAG EDYLIVAEDL VETLTETLES SFKVIKTLPG 300
    KALENSTYQH PLFEREGPLV LGDYVTTESG TGLVHTAPGH GQEDYQVGQQ 350
    YGLAMLSPVD GDGTFTDEAG PFAGLNVLNG GNEAVIEALQ SAGALLKEES 400
    YAHKYPYDWR TKKPVILRAT EQWFASVDGF REAVLDAIAT VNWIPAQGEN 450
    RITSMVSERS DWCISRQRNW GVPIPVFYND ATGEPLLNEA TVNHIQGIVA 500
    EKGSDAWWEL DNDELLPEPY RSDGNTYRKG TDTMDVWFDS GSSWAAVCDQ 550
    REPLKYPAEM YLEGSDQHRG WFQSSILTSV ATNGHAPYKT VLTHGFVLDE 600
    QGRKMSKSIG NVVDPAIVIA GGKNQKQDPP YGADVLRLWV SSVDYASDVP 650
    LGKNILKQMA DVYRKIRNTS RFLLGNLHDF DPAQDAVAYE DLPQLDRYML 700
    HRITEVFTDV TEAFESFQFF RFFQTVQNFC VVDLSNFYLD IAKDRLYISE 750
    PNAQRRRSCQ TVLAIALENL ARAIAPVLSH MAEDIWQSLP YETEHQSVFA 800
    SGWMRLEDQW HNPDLASHWI VLREIRQEVN KVLEQARTEK EIGSSLEAKV 850
    LLYVSDTDLR QQLDAMNPST GGGSNNVDEL RYLFLASQVE LLETPKNLDR 900
    LMYQFQSETL GVGVVTADGK KCDRCWNYST YVGRSKQHPL LCDRCEPIIE 950
    NLVTQGQISL TEEGRYQPNT K 971
    Length:971
    Mass (Da):109,792
    Last modified:February 26, 2008 - v1
    Checksum:i206CB68F80AB54D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000828 Genomic DNA. Translation: ABW28892.1.
    RefSeqiYP_001518209.1. NC_009925.1.

    Genome annotation databases

    EnsemblBacteriaiABW28892; ABW28892; AM1_3907.
    GeneIDi5682711.
    KEGGiamr:AM1_3907.
    PATRICi20621992. VBIAcaMar40141_3622.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000828 Genomic DNA. Translation: ABW28892.1 .
    RefSeqi YP_001518209.1. NC_009925.1.

    3D structure databases

    ProteinModelPortali B0C872.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 329726.AM1_3907.

    Proteomic databases

    PRIDEi B0C872.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABW28892 ; ABW28892 ; AM1_3907 .
    GeneIDi 5682711.
    KEGGi amr:AM1_3907.
    PATRICi 20621992. VBIAcaMar40141_3622.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci AMAR329726:GCZJ-3894-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MBIC 11017.

    Entry informationi

    Entry nameiSYI_ACAM1
    AccessioniPrimary (citable) accession number: B0C872
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3