Reviewed, UniProtKB/Swiss-Prot B0C6R3 (PURL_ACAM1)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase 2
    EC=6.3.5.3
Alternative name(s):
    Phosphoribosylformylglycinamidine synthase II
      Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	AM1_2612

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	783 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, purQ and purL By similarity. HAMAP MF_00420
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 783	783	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000080545
Regions
	Nucleotide binding	107 – 118	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B0C6R3-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 8587EAA598D03B3F	FASTA	783	84,126
	10 20 30 40 50 60 MASPGSSPFS PEEIATEGLK PEEYDDIVQR LGRHPNRAEL GMFGVMWSEH CCYKNSRPLL 70 80 90 100 110 120 SQFPTEGKHV LVGPGENAGV VDLGNGLQLA FKVESHNHPS AVEPFQGAAT GVGGILRDIF 130 140 150 160 170 180 TMGARPIAVL NSLRFGDLDD ARTRRLFSGV VAGIAHYGNC VGVPTVGGEV YFDPAYTGNP 190 200 210 220 230 240 LVNAMAMGLM ETAEIVKAGA SGVGNPVLYV GSTTGRDGMG GASFASAELD ATSEVEDRPA 250 260 270 280 290 300 VQVGDPFLEK SLIEACLEAF QTGAVVAAQD MGAAGLTCST AEMAEKGGVG IDLDLDLIPN 310 320 330 340 350 360 RETGMVPYEY LLSESQERML FVAEKGREQE LIDIFHRWGL QAVVAGSVIA EPIVKIRFQG 370 380 390 400 410 420 EVAAEIPATA LSDNTPIYKR EVLAEPPEYV QKAREWSCDR IPPGTAEGIE IQGKLNSWSD 430 440 450 460 470 480 ILLQLLQVPT IASKRWVYRQ YDHQVQNNTV VLPGDADATI VRLRPVAHQE TPDQGVDSKT 490 500 510 520 530 540 AVAATIDCNS RYVYLDPYEG AKMTVAEAAR NLSCVGAEPI AVTDNLNFGS PEKPIGYWQL 550 560 570 580 590 600 AEACRGLSEA CRHLQTPVTG GNVSLYNETF DADGQPQPIY PTPVIGMVGL VPDLTKTCVQ 610 620 630 640 650 660 GWQADGDLIY LLGIPLDVQS DLVTLGATEY LASIHHQVAG EPPHADLDLE AQVQATCRHG 670 680 690 700 710 720 IQQGWLHSAH DCAEGGVAVA LAESCISGQR GADIQVPTGS NRWDQVLFGE GGARIVISVS 730 740 750 760 770 780 ATHQAAWESY LQEHLPQYWQ QIGTVTTPNS ELTLTTNVHQ TLIKVRIEEA TQRWAQAIES KLA

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000828 Genomic DNA. Translation: ABW27619.1.
RefSeq	YP_001516933.1.
3D structure databases
SMR	B0C6R3. Positions 21-721.
ModBase	Search...
Genome annotation databases
GeneID	5681424.
GenomeReviews	Gene locus AM1_2612 in contig CP000828_GR.
KEGG	amr:AM1_2612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	GNCVGVP.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ACAM1

Accession

Primary (citable) accession number: B0C6R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)