B0C4N8 (ISPH_ACAM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	AM1_4950

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Acaryochloris

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000077510

Sequences

	Sequence		Length	Mass (Da)
	B0C4N8 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 28C563C9D2F03FF9	FASTA	415	46,697
	10 20 30 40 50 60 MDTKAFKRSL HKSEQYHRKG FGHEEEVTNL LQSEYNSKLI QQIRNNGYRL QQGDVTIHLA 70 80 90 100 110 120 EAFGFCWGVE RAIALAYETR RQFPQERIWI TNEIIHNPSV NKNLRDMKVE FIPVQPDGQK 130 140 150 160 170 180 DFTVVGQNDV VILPAFGASV QEMQLLHDKG CTIMDTTCPW VSKVWTSVEK HKKGSYTSII 190 200 210 220 230 240 HGKYKHEETI ATSSFAGTYL VLLNLEEAEY VADYILNPGD TPSEREQRRA DFMAKFANAH 250 260 270 280 290 300 SPGFDPETDL ERVGIANQTT MLKGETEQIG KLFERTVMRK YGPAQVNEHF LSFNTICDAT 310 320 330 340 350 360 QERQDAIFQL VDEPLDLMVV IGGFNSSNTT HLQEISIDKN IPSYHIDSVD RIGPGNRVEH 370 380 390 400 410 KPLNADLTVT DNWLPNGPLA IGVTSGASTP DKVVSDIVEK IFSIKSASAE VVGVR

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBIC 11017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000828 Genomic DNA. Translation: ABW29921.1.
RefSeq	YP_001519239.1. NC_009925.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B0C4N8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5683750.
GenomeReviews	Gene locus AM1_4950 in contig CP000828_GR.
KEGG	amr:AM1_4950.
PATRIC	20623950. VBIAcaMar40141_4592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286221.
OMA	VQEMQLL.
PhylomeDB	B0C4N8.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	AMAR329726:AM1_4950-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_ACAM1

Accession

Primary (citable) accession number: B0C4N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program