B0C4G1 (KATG_ACAM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	AM1_3715

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Acaryochloris

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	Catalase-peroxidase HAMAP MF_01961		PRO_0000354707
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0C4G1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: F2517EA8C0785018	FASTA	732	80,691
	10 20 30 40 50 60 MSSASKCPFS GGALKFTAGS GTANRDWWPN QLNLQILRQH SPKSNPMDKA FNYAEAFKSL 70 80 90 100 110 120 DLADVKQDIF DLMKSSQDWW PADYGHYGPL FIRMAWHSAG TYRIGDGRGG AGTGNQRFAP 130 140 150 160 170 180 INSWPDNANL DKARMLLWPI KQKYGAKISW ADLMILAGNC ALESMGFKTF GFAGGREDIW 190 200 210 220 230 240 EPEEDIYWGA ETEWLGDQRY TGDRDLEATL GAVQMGLIYV NPEGPNGHPD PVASGRDIRE 250 260 270 280 290 300 TFGRMAMNDE ETVALTAGGH TFGKCHGAGD DAHVGPEPEG ARIEDQCLGW KSSFGTGKGV 310 320 330 340 350 360 HAITSGIEGA WTTNPTQWDN NYFENLFGYE WELTKSPAGA NQWVPQGGAG ANTVPDAHDP 370 380 390 400 410 420 SRRHAPIMTT ADMAMRMDPI YSPISRRFLD NPDQFADAFA RAWFKLTHRD MGPRSRYLGP 430 440 450 460 470 480 EVPEEELIWQ DPVPAVNHEL INEQDIATLK SQILATNLTV SQLVSTAWAS AVTYRNSDKR 490 500 510 520 530 540 GGANGARIRL APQRDWEVNQ PAQLATVLQT LEAVQTTFNH SQIGGKRVSL ADLIVLGGCA 550 560 570 580 590 600 GVEQAAKNAG WYDVKVPFKP GRTDATQAQT DVTSFAVLEP RADGFRNYLK GHYPVSAEEL 610 620 630 640 650 660 LVDKAQLLTL TAPEMTVLVG GLRVLNANVG QAQHGVFTHR PESLTNDFFL NLLDMSVTWA 670 680 690 700 710 720 ATSEAEEVFE GRDRKTGALK WTGTRVDLIF GSNSQLRALA EVYGCEDSQQ RFVQDFVAAW 730 DKVMNLDRFD LA

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBIC 11017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000828 Genomic DNA. Translation: ABW28705.1.
RefSeq	YP_001518021.1. NC_009925.1.
3D structure databases
ProteinModelPortal	B0C4G1.
SMR	B0C4G1. Positions 19-732.
ModBase	Search...
Protein-protein interaction databases
STRING	B0C4G1.
Protein family/group databases
PeroxiBase	6248. ACmaCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5682521.
GenomeReviews	Gene locus AM1_3715 in contig CP000828_GR.
KEGG	amr:AM1_3715.
PATRIC	20621646. VBIAcaMar40141_3451.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	B0C4G1.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	AMAR329726:AM1_3715-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ACAM1

Accession

Primary (citable) accession number: B0C4G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program