Reviewed, UniProtKB/Swiss-Prot B0C1R1 (SYL_ACAM1)

Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	AM1_1037

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	855 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 855	855	Leucyl-tRNA synthetase HAMAP MF_00049		PRO_1000074823
Regions
	Motif	42 – 52	11	"HIGH" region HAMAP MF_00049
	Motif	614 – 618	5	"KMSKS" region HAMAP MF_00049
Sites
	Binding site	617	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0C1R1-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 6BEA950C874A35F9	FASTA	855	95,949
	10 20 30 40 50 60 MESRYNPTEI EPKWQASWAK QGLDATPEDT SKPKFYALSM FPYPSGSLHV GHTRNYVITD 70 80 90 100 110 120 VIARLKRMQG YRVLQPMGWD AFGLPAENAA IARGIHPAEW TYANMAQMKK QLEPLGLSID 130 140 150 160 170 180 WSREIATCSP DYYRWTQWIF LQFLDMGLAY QKEAAVNWDP VDQTVLANEQ VDNEGRSWRS 190 200 210 220 230 240 GAKVERKLLR QWFLKITDYA EELLTDLDKL TGWPERVKTM QANWIGKSVG AYLEFPIVGM 250 260 270 280 290 300 DEKVAVFTTR PDTVYGVTYV VLAPEHPLTP QVTSKAQKKA VDKFIEAVGA ESEMDRTAED 310 320 330 340 350 360 KPKKGIPTGG KAINPFTGEE IPIWIADYVL YEYGTGAVMG VPAHDTRDFV FAQQNKLPIQ 370 380 390 400 410 420 TVIVPEGGDA ETPLEAAYTE PGLMVNSGEF DGKVSTEGKQ AIIAKAETKG WGKARVQYRL 430 440 450 460 470 480 RDWLISRQRY WGVPIPVIHC PNCGAVPVPE ADLPVELPED VEFSAQGGSP LAKLESWVNV 490 500 510 520 530 540 ACPNCGADAK RETDTMDTFI DSSWYFLRYP DAKNDKAVFD SAKTNDWLPV DQYVGGIEHA 550 560 570 580 590 600 ILHLLYSRFF TKVMRDRKLL NFDEPFKKLL TQGMVQALTY KNPETGQYIA PINVDADDPK 610 620 630 640 650 660 DPETGAPLEA FYEKMSKSKY NGVPPEEVTN KYGADTARLF TLFKAPPEKD LEWEGADVEG 670 680 690 700 710 720 QFRFLNRVWR LVSEHAAQAK GKKAKVNKAK LSKTEKELRR SIHIAIKETS EDLDGDYQFN 730 740 750 760 770 780 TAVSELMKLS NALSDSKEKA SPVYAEGVET LLLLLTPFAP HISEELWENL GHSESILGQT 790 800 810 820 830 840 WPQVDEEALV ADEITLVIQI MGKTRGTIQV PAGSSREDLE QLARESEVAQ RYIAGKEVKK 850 VIVVPGKLVN FVVPK

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW26077.1.
RefSeq	YP_001515391.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5679862.
GenomeReviews	Gene locus AM1_1037 in contig CP000828_GR.
KEGG	amr:AM1_1037.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0C1R1. YYLRFID.
Family and domain databases
HAMAP	MF_00049. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_ACAM1

Accession

Primary (citable) accession number: B0C1R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)