B0C1B5 (PROB_ACAM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	AM1_4676

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Acaryochloris

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Glutamate 5-kinase HAMAP MF_00456		PRO_1000081034
Regions
	Domain	278 – 356	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	B0C1B5 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 2B1391A45EEAE2F2	FASTA	371	39,423
	10 20 30 40 50 60 MSQTLVLKIG TSSLTNPDTG HLALATIASL VEVLSQLRQE GNQVILVSSG AVGIGCARLG 70 80 90 100 110 120 LAERPKAIAL KQAVAAVGQG RLMRIYDDFF TSLQQPIAQV LLTRGDLADR SRYINASNTL 130 140 150 160 170 180 QELLKLGVIP IVNENDTVAV DELLRFGDND TLSALVAGLV QADWLFILTD VDRLYSADPR 190 200 210 220 230 240 IQPDAQPIVT VERMEELAAL NVNAGAQGSQ WGTGGMATKI SAAAIATNAG VRTVITQGKT 250 260 270 280 290 300 PENIPRILAG ESLGTQFQPH PRPHNARKHW IAHALVPTGK LILDDGAAKA ITTLGKSLLA 310 320 330 340 350 360 AGITAVEGEF QSQEAVCFYD SAGVEIARGL VNYSSDELQR IQGRQSEDIP QVLGYAGAET 370 VVHRDNLVIT G

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBIC 11017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000828 Genomic DNA. Translation: ABW29650.1.
RefSeq	YP_001518968.1. NC_009925.1.
3D structure databases
ProteinModelPortal	B0C1B5.
ModBase	Search...
Protein-protein interaction databases
STRING	B0C1B5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5683478.
GenomeReviews	Gene locus AM1_4676 in contig CP000828_GR.
KEGG	amr:AM1_4676.
PATRIC	20623426. VBIAcaMar40141_4331.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507643.
OMA	TFGDNDM.
PhylomeDB	B0C1B5.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	AMAR329726:AM1_4676-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ACAM1

Accession

Primary (citable) accession number: B0C1B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program