B0BZI8 (GLO2_ACAM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	AM1_5788

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Acaryochloris

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000087274
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	137	1	Zinc 1 By similarity
	Metal binding	137	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BZI8 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 17620DDCCE37E58E	FASTA	257	29,006
	10 20 30 40 50 60 MQIHRLPAFS DNYIFVLHDP GQNIAAVVDP ADPQPVLKKL AELGAELVAI FNTHHHSDHV 70 80 90 100 110 120 GGNRTLLQAF PNTVVYGGEQ DRGRIPGQQH FLKEGDQVSF AHRRAEVYFV PGHTRAHIAY 130 140 150 160 170 180 YFPPTTGEEW GELFCGDTLF AGGCGRLFEG TPAQMVDSLS KLRNLPETTR VWCAHEYTLK 190 200 210 220 230 240 NLQFALTVDS DNSHLKNRFK QVQNARNLSQ PTVPSDIGLE KQTNPFLRWD QPHLIIHTKS 250 NTPVQCFARL RGMKDQF

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MBIC 11017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000828 Genomic DNA. Translation: ABW30733.1.
RefSeq	YP_001520052.1. NC_009925.1.
3D structure databases
ProteinModelPortal	B0BZI8.
ModBase	Search...
Protein-protein interaction databases
STRING	B0BZI8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5684575.
GenomeReviews	Gene locus AM1_5788 in contig CP000828_GR.
KEGG	amr:AM1_5788.
PATRIC	20625538. VBIAcaMar40141_5374.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	B0BZI8.
Enzyme and pathway databases
BioCyc	AMAR329726:AM1_5788-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_ACAM1

Accession

Primary (citable) accession number: B0BZI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program