Reviewed, UniProtKB/Swiss-Prot B0BYR8 (SYS_ACAM1)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	serS
Ordered Locus Names:	AM1_2069

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Seryl-tRNA synthetase HAMAP MF_00176		PRO_1000077183
Regions
	Nucleotide binding	264 – 266	3	ATP By similarity
	Nucleotide binding	351 – 354	4	ATP By similarity
	Region	233 – 235	3	Serine binding By similarity
Sites
	Binding site	287	1	Serine By similarity
	Binding site	387	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BYR8-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 5956B09FC385E842	FASTA	424	47,218
	10 20 30 40 50 60 MIDLKQLREN PEAFAECLSR RGDYDLQAIL DLDQKQRELE TERSQLQARS NQIGKTIGER 70 80 90 100 110 120 MKGGANPKDP EIQNLRQEGS TVKATLSDLE PKERDLKAEI GELLLTIPNL PSDDTPVGKD 130 140 150 160 170 180 ETANVEVSRW GDEYIPKNAK LAHWEIGENL GILNFERSVK VAQSRFATLV GAGAALERAL 190 200 210 220 230 240 IQFMLDQQVE AGYVEVLPPV LVNSDSLTAT GQLPKFAEES FKCAEDDLWL SPTAEVPVTN 250 260 270 280 290 300 LYRDEIINAD DLPIFHCAYT PCFRREAGSY GRDTRGLIRL HQFNKVELVK LVHPSTSAAE 310 320 330 340 350 360 HEALVKNAAA ILEALKLPYR VLQLCTGDLG FSAAKCYDLE VWLPSADCYR EISSCSNFLD 370 380 390 400 410 420 FQARRGNIRF KESGQKGTQF VHTLNGSGLA VGRTMAAVLE NYQQPDGSVQ VPDVLQPYLK RKVL

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000828 Genomic DNA. Translation: ABW27084.1.
RefSeq	YP_001516398.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5680883.
GenomeReviews	Gene locus AM1_2069 in contig CP000828_GR.
KEGG	amr:AM1_2069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LKPYMGG.
Family and domain databases
HAMAP	MF_00176. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_ACAM1

Accession

Primary (citable) accession number: B0BYR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)