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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Rickettsia rickettsii (strain Iowa)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotationSAAS annotation

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (RrIowa_1371)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei180Proton donor/acceptorUniRule annotation1
Binding sitei181SubstrateUniRule annotation1
Active sitei184Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 59NAD(P)UniRule annotation6
Nucleotide bindingi124 – 126NAD(P)UniRule annotation3
Nucleotide bindingi148 – 151NAD(P)UniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotationImported

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

NADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotationSAAS annotation (EC:1.17.1.8UniRule annotationSAAS annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:RrIowa_0233Imported
OrganismiRickettsia rickettsii (strain Iowa)Imported
Taxonomic identifieri452659 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000000796 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0BWB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 151DapB_NInterPro annotationAdd BLAST103
Domaini154 – 282DapB_CInterPro annotationAdd BLAST129

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 191Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapB family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000227155.
KOiK00215.
OMAiIEEYPKK.
OrthoDBiPOG091H01P6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB. 1 hit.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR023940. DHDPR_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BWB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASYNKICGS NYIKYTLRSK DFRRLALVQI KLRLLTVHLL GVYIANMLNI
60 70 80 90 100
GLSGSTGKMG ETILERIDKF KDCKIAAKFN STNNLDDLDN FCKNSDVIID
110 120 130 140 150
FSTPEILEKL INYALKHNTK LVIGTTGLQP QHFKLLEKAA QTLPVLYSAN
160 170 180 190 200
MSIGANLLSY LAKEVTKILD DYDVEILETH HRNKKDSPSG TAVMLAETIA
210 220 230 240 250
SKKGLNITFN RGNRLRSEKE IGISSLRGGN VHGIHEISFL GDDEIITLKH
260 270 280
EALNKNSFSI GAIKAAIWLQ DKPSALYSMQ DIYKI
Length:285
Mass (Da):31,820
Last modified:February 26, 2008 - v1
Checksum:i549827E6CE4F0EA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000766 Genomic DNA. Translation: ABY72145.1.

Genome annotation databases

EnsemblBacteriaiABY72145; ABY72145; RrIowa_0233.
KEGGirrj:RrIowa_0233.
PATRICi17906348. VBIRicRic59104_0227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000766 Genomic DNA. Translation: ABY72145.1.

3D structure databases

ProteinModelPortaliB0BWB9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY72145; ABY72145; RrIowa_0233.
KEGGirrj:RrIowa_0233.
PATRICi17906348. VBIRicRic59104_0227.

Phylogenomic databases

HOGENOMiHOG000227155.
KOiK00215.
OMAiIEEYPKK.
OrthoDBiPOG091H01P6.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB. 1 hit.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR023940. DHDPR_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB0BWB9_RICRO
AccessioniPrimary (citable) accession number: B0BWB9
Entry historyi
Integrated into UniProtKB/TrEMBL: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.