B0BW84 (RNC_RICRO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	RrIowa_0197

Organism

Rickettsia rickettsii (strain Iowa) [Complete proteome] [HAMAP]

Taxonomic identifier

452659 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia rickettsii

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000075804
Regions
	Domain	4 – 133	130	RNase III
	Domain	158 – 226	69	DRBM
Sites
	Active site	50	1	Potential
	Active site	122	1	By similarity
	Metal binding	46	1	Magnesium By similarity
	Metal binding	119	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BW84 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: E7481B1E507843A7	FASTA	227	26,067
	10 20 30 40 50 60 MESFEKLEKL LSYSFKNKEL LIEALSHPSL RQHHEYKDDK DYERLEFLGD AVLNLVITEI 70 80 90 100 110 120 LFRNFANYNE GNLAKIRSYL VCKETICMVG AKLTLKNYII MTHGEEVAGG RDNLNNIENA 130 140 150 160 170 180 TEALIAAIYL DSNIETTHDI IEKLWAEFIK VQNLTDYDPK TALQEWAQAS DHHLPIYRLI 190 200 210 220 KREGASHSST FTVLVKVKDY EQTGTGHAIK EAEKNAARSL LHRLKND

References

[1]

"Genomic comparison of virulent Rickettsia rickettsii Sheila Smith and avirulent Rickettsia rickettsii Iowa." Ellison D.W., Clark T.R., Sturdevant D.E., Virtaneva K., Porcella S.F., Hackstadt T. Infect. Immun. 76:542-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Iowa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000766 Genomic DNA. Translation: ABY72110.1.
RefSeq	YP_001649516.1. NC_010263.2.
3D structure databases
ProteinModelPortal	B0BW84.
ModBase	Search...
Protein-protein interaction databases
STRING	452659.RrIowa_0197.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY72110; ABY72110; RrIowa_0197.
GeneID	5850312.
KEGG	rrj:RrIowa_0197.
PATRIC	17906276. VBIRicRic59104_0192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246808.
KO	K03685.
OMA	LTHKSCK.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	RRIC452659:GHSN-188-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_RICRO

Accession

Primary (citable) accession number: B0BW84

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program