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B0BUD5

- PNP_ACTPJ

UniProt

B0BUD5 - PNP_ACTPJ

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, APJL_0570
Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851Magnesium By similarity
Metal bindingi491 – 4911Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciAPLE434271:GIX7-564-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:APJL_0570
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000008547: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_1000147877Add
BLAST

Proteomic databases

PRIDEiB0BUD5.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi434271.APJL_0570.

Structurei

3D structure databases

ProteinModelPortaliB0BUD5.
SMRiB0BUD5. Positions 616-690.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61160KH
Add
BLAST
Domaini621 – 68969S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BUD5-1 [UniParc]FASTAAdd to Basket

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MNPIVKQFKY GQHTVTLETG AIARQATAAV MASMDDTTVF VTVVAKKEVK    50
EGQDFFPLTV DYQERTYAAG RIPGGFFKRE GRPSEGETLI ARLIDRPVRP 100
LFPEGFFNEI QVIATVVSVN PQISPDLVAM IGASAALSLS GVPFNGPIGA 150
ARVGFINDQF VLNPTTSEQK ISRLDLVVAG TDKAVLMVES EADILSEEQM 200
LSAVVFGHQQ QQVVIENIKE FVKEAGKPRW DWVAPEPNTA LINQVKALAE 250
ARIGDAYRIT EKQARYEQID AIKADVIAQL TTQDETISEG AIIDIITALE 300
SSIVRGRIIA GEPRIDGRTV DTVRALDICT GVLPRTHGSA IFTRGETQAL 350
AVATLGTERD AQIIDELTGE KSDRFLFHYN FPPYSVGETG RIGSPKRREI 400
GHGRLAKRGV LAVMPTAEEF PYVVRVVSEI TESNGSSSMA SVCGASLALM 450
DAGVPIKAAV AGIAMGLVKE EEKFVVLSDI LGDEDHLGDM DFKVAGTREG 500
VTALQMDIKI EGITPEIMQI ALNQAKGARM HILSVMEQAI PAPRADISDF 550
APRIHTMKID PKKIKDVIGK GGAVIRALTE ETGTSIDIDD DGTVKIAATD 600
NNAAKAVMAR IEDIVAEVEV NAIYKGKVTR VVDFGAFVSI LGGKEGLVHI 650
SQITNERVER VADYLSVGQE VTVKVVEIDR QNRIRLTMKD LNNDTPVAEN 700
VTEEAEVSSE QQAEI 715
Length:715
Mass (Da):77,441
Last modified:February 26, 2008 - v1
Checksum:i7D45A21746CC9877
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000687 Genomic DNA. Translation: ABY69140.1.
RefSeqiWP_012262862.1. NC_010278.1.
YP_001651584.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY69140; ABY69140; APJL_0570.
GeneIDi5851945.
KEGGiapj:APJL_0570.
PATRICi20750376. VBIActPle136345_0574.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000687 Genomic DNA. Translation: ABY69140.1 .
RefSeqi WP_012262862.1. NC_010278.1.
YP_001651584.1. NC_010278.1.

3D structure databases

ProteinModelPortali B0BUD5.
SMRi B0BUD5. Positions 616-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 434271.APJL_0570.

Proteomic databases

PRIDEi B0BUD5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY69140 ; ABY69140 ; APJL_0570 .
GeneIDi 5851945.
KEGGi apj:APJL_0570.
PATRICi 20750376. VBIActPle136345_0574.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci APLE434271:GIX7-564-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
    Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T.
    , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
    PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JL03.

Entry informationi

Entry nameiPNP_ACTPJ
AccessioniPrimary (citable) accession number: B0BUD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 26, 2008
Last modified: September 3, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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