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B0BTN8 (RIBBA_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Riboflavin biosynthesis protein ribBA

Including the following 2 domains:

  1. 3,4-dihydroxy-2-butanone 4-phosphate synthase
    Short name=DHBP synthase
    EC=4.1.99.12
  2. GTP cyclohydrolase-2
    EC=3.5.4.25
    Alternative name(s):
    GTP cyclohydrolase II
Gene names
Name:ribBA
Ordered Locus Names:APJL_0404
OrganismActinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]
Taxonomic identifier434271 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_01283

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP MF_01283

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate. HAMAP MF_01283

Cofactor

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01283

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283

Sequence similarities

In the N-terminal section; belongs to the DHBP synthase family.

In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Riboflavin biosynthesis protein ribBA HAMAP MF_01283
PRO_1000140359

Regions

Nucleotide binding254 – 2585GTP By similarity
Nucleotide binding297 – 2993GTP By similarity
Region1 – 203203DHBP synthase HAMAP MF_01283
Region30 – 312D-ribulose 5-phosphate binding By similarity
Region142 – 1465D-ribulose 5-phosphate binding By similarity
Region204 – 401198GTP cyclohydrolase II HAMAP MF_01283

Sites

Active site3311Proton acceptor; for GTP cyclohydrolase activity Potential
Active site3331Nucleophile; for GTP cyclohydrolase activity By similarity
Metal binding311Magnesium or manganese 1 By similarity
Metal binding311Magnesium or manganese 2 By similarity
Metal binding1451Magnesium or manganese 2 By similarity
Metal binding2591Zinc; catalytic By similarity
Metal binding2701Zinc; catalytic By similarity
Metal binding2721Zinc; catalytic By similarity
Binding site351D-ribulose 5-phosphate By similarity
Binding site1661D-ribulose 5-phosphate By similarity
Binding site2751GTP By similarity
Binding site3191GTP By similarity
Binding site3541GTP By similarity
Binding site3591GTP By similarity
Site1281Essential for DHBP synthase activity By similarity
Site1661Essential for DHBP synthase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
B0BTN8 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: D51B681702C4D209

FASTA40144,673
        10         20         30         40         50         60 
MTDFQFSKVE DAIEAIRQGK IILVTDDEDR ENEGDFICAA EFATPENINF MATYGKGLIC 

        70         80         90        100        110        120 
TPISTEIAKK LNFHPMVAVN QDNHETAFTV SVDHIDTGTG ISAFERSITA MKIVDDNAKA 

       130        140        150        160        170        180 
TDFRCPGHMF PLIAKDGGVL VRNGHTEATV DLARLAGLKH AGLCCEIMAD DGTMMTMPDL 

       190        200        210        220        230        240 
QKFAVEHNMP FITIQQLQEY RRKHDSLVKQ ISVVKMPTKY GEFMAHSFVE VISGKEHVAL 

       250        260        270        280        290        300 
VKGDLTDGEQ VLARIHSECL TGDAFGSQRC DCGQQFAAAM TQIEQEGRGV ILYLRQEGRG 

       310        320        330        340        350        360 
IGLINKLRAY ELQDKGMDTV EANVALGFKE DEREYYIGAQ MFQQLGVKSI RLLTNNPAKI 

       370        380        390        400 
EGLKEQGLNI VAREPIIVEP NKNDIDYLKV KQIKMGHMFN F

« Hide

References

[1]"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. expand/collapse author list , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JL03.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000687 Genomic DNA. Translation: ABY68993.1.
RefSeqYP_001651437.1. NC_010278.1.

3D structure databases

ProteinModelPortalB0BTN8.
SMRB0BTN8. Positions 208-378.
ModBaseSearch...

Protein-protein interaction databases

STRINGB0BTN8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5851822.
GenomeReviewsGene locus APJL_0404 in contig CP000687_GR.
KEGGapj:APJL_0404.
PATRIC20750035. VBIActPle136345_0406.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG735778.
OMAPRFANTC.
ProtClustDBCLSK918908.

Enzyme and pathway databases

BioCycAPLE434271:APJL_0404-MONOMER.

Family and domain databases

HAMAPMF_01283. RibBA.
[Tree]
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
Gene3DG3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KOK14652.
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
SUPFAMSSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMsTIGR00505. RibA. 1 hit.
TIGR00506. RibB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRIBBA_ACTPJ
AccessionPrimary (citable) accession number: B0BTN8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 26, 2008
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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