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B0BTK6 (TPIS_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1
Alternative name(s):
Triose-phosphate isomerase
Gene names
Name:tpiA
Ordered Locus Names:APJL_1972
OrganismActinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]
Taxonomic identifier434271 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B

Subunit structure

Homodimer By similarity. HAMAP MF_00147_B

Subcellular location

Cytoplasm By similarity HAMAP MF_00147_B.

Sequence similarities

Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
Pentose shunt
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

glycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

pentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontriose-phosphate isomerase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256Triosephosphate isomerase HAMAP MF_00147_B
PRO_1000096472

Sites

Active site971Electrophile By similarity
Active site1691Proton acceptor By similarity
Binding site101Substrate By similarity
Binding site121Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B0BTK6 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: 273D2543F1588216

FASTA25626,522
        10         20         30         40         50         60 
MARRPLVMGN WKLNGSKAFT KELIAGLKAE LADVKGCDVA IAPPVMYLAE AEAALAGQSV 

        70         80         90        100        110        120 
IALGAQNVDV NVQGAFTGDI STEMLKDFGA KYIIIGHSER RTYHKECDTF IAKKFAALKA 

       130        140        150        160        170        180 
AGLVPVLCIG ETEAENEAGQ TEAVCAKQID AVIDALGVEA FNGAVIAYEP IWAIGTGKSA 

       190        200        210        220        230        240 
TPAQAQAVHA FIRGHIAAKS QAVADQVIIQ YGGSVNDANA AELFTQPDID GALVGGASLK 

       250 
APAFAVIVKA AEKAKA

« Hide

References

[1]"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. expand/collapse author list , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JL03.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000687 Genomic DNA. Translation: ABY70520.1.
RefSeqYP_001652964.1. NC_010278.1.

3D structure databases

ProteinModelPortalB0BTK6.
SMRB0BTK6. Positions 3-255.
ModBaseSearch...

Protein-protein interaction databases

STRINGB0BTK6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5851232.
GenomeReviewsGene locus APJL_1972 in contig CP000687_GR.
KEGGapj:APJL_1972.
PATRIC20753296. VBIActPle136345_1965.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG708281.
OMAVCVGETQ.
ProtClustDBPRK00042.

Enzyme and pathway databases

BioCycAPLE434271:APJL_1972-MONOMER.

Family and domain databases

HAMAPMF_00147_B. TIM_B.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01803.
PANTHERPTHR21139. Triophos_ismrse. 1 hit.
PfamPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMSSF51351. Triophos_ismrse. 1 hit.
TIGRFAMsTIGR00419. Tim. 1 hit.
PROSITEPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTPIS_ACTPJ
AccessionPrimary (citable) accession number: B0BTK6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 26, 2008
Last modified: January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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