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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei97ElectrophileUniRule annotation1
Active sitei169Proton acceptorUniRule annotation1
Binding sitei175Substrate; via amide nitrogenUniRule annotation1
Binding sitei214SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomeraseUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIMUniRule annotation
Short name:
TPIUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotation
Ordered Locus Names:APJL_1972
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000008547 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000964721 – 256Triosephosphate isomeraseAdd BLAST256

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0BTK6.
SMRiB0BTK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 12Substrate bindingUniRule annotation3
Regioni235 – 236Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226413.
KOiK01803.
OMAiLCVGEGL.
OrthoDBiPOG091H02GL.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR035990. TIM_sf.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiView protein in Pfam
PF00121. TIM. 1 hit.
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiView protein in PROSITE
PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B0BTK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRPLVMGN WKLNGSKAFT KELIAGLKAE LADVKGCDVA IAPPVMYLAE
60 70 80 90 100
AEAALAGQSV IALGAQNVDV NVQGAFTGDI STEMLKDFGA KYIIIGHSER
110 120 130 140 150
RTYHKECDTF IAKKFAALKA AGLVPVLCIG ETEAENEAGQ TEAVCAKQID
160 170 180 190 200
AVIDALGVEA FNGAVIAYEP IWAIGTGKSA TPAQAQAVHA FIRGHIAAKS
210 220 230 240 250
QAVADQVIIQ YGGSVNDANA AELFTQPDID GALVGGASLK APAFAVIVKA

AEKAKA
Length:256
Mass (Da):26,522
Last modified:February 26, 2008 - v1
Checksum:i273D2543F1588216
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY70520.1.
RefSeqiWP_005599672.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY70520; ABY70520; APJL_1972.
GeneIDi4848635.
KEGGiapj:APJL_1972.

Similar proteinsi

Entry informationi

Entry nameiTPIS_ACTPJ
AccessioniPrimary (citable) accession number: B0BTK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 26, 2008
Last modified: March 28, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome