Reviewed, UniProtKB/Swiss-Prot B0BTA2 (SYM_ACTPJ)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	APJL_0368

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 682	682	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_1000093694
Regions
	Domain	580 – 682	103	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	328 – 332	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	331	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BTA2-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: AACE9EB6A304E07A	FASTA	682	77,452
	10 20 30 40 50 60 MSRKMLVTCA LPYANGAIHL GHMLEHIQAD IWVRFQRMRG NEIYFVCADD AHGTPIMLNA 70 80 90 100 110 120 AKQGITPEQL IEKAKTDHVA DFKGFNISFD NYHSTHSEEN REITTEMYKK LRANGFIKSR 130 140 150 160 170 180 VISQLFDPEK QMFLPDRFVK GTCPKCKAED QYGDNCEVCA STYSPMDLIN PRSAVSGATP 190 200 210 220 230 240 IVKESEHFFF DLPSFEGMLK EWTRSGSLQS EITNKMQEWF ESGLQQWDIS RDAPYFGFPI 250 260 270 280 290 300 PDAENKFFYV WLDAPIGYMA SFKNLCDRTG LNFDEFWKKD SETELYHFIG KDIVYFHSLF 310 320 330 340 350 360 WPAMLDGCEL RKPTNVFAHG YVTVDGVKMS KSRGTFIQAS TYLKHIDPEC LRYYYAAKLN 370 380 390 400 410 420 ERIEDLDLSL EDFVQRVNSD IVNKLVNLAS RNASFIAKRF EGKLADKLED EALFAEFIAQ 430 440 450 460 470 480 SEQIAAHYEN REFNKAIRLI MDLCDKANKY VDDKAPWVIA KQEGCDAQLQ AVCSMGIELF 490 500 510 520 530 540 RVLMSYLKPV LPQLAERAEA FLQTELTWDN IQQPLLGQNV APFKSLFSRL EKKQIDAVIE 550 560 570 580 590 600 ETKALFAAQN KAEDKKGKQK VENTENTAVE PIAAEITIDD FAKLDLRVAK VISCEAVPES 610 620 630 640 650 660 NKLLKFQLDL GDHQRQVLSG IKAAYNNPEE LVGRFVIMVA NLAPRKMKFG VSEGMILSAG 670 680 TGGADLFLLS ADEGIRPGMQ VK

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY68959.1.
RefSeq	YP_001651403.1.
3D structure databases
SMR	B0BTA2. Positions 2-543, 3-642, 576-681.
ModBase	Search...
Genome annotation databases
GeneID	5851781.
GenomeReviews	Gene locus APJL_0368 in contig CP000687_GR.
KEGG	apj:APJL_0368.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	CEAVPES.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ACTPJ

Accession

Primary (citable) accession number: B0BTA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)