Reviewed, UniProtKB/Swiss-Prot B0BT67 (PYRE_ACTPJ)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	APJL_0333

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000138758
Regions
	Region	34 – 35	2	Orotate binding By similarity
	Region	72 – 73	2	5-phosphoribose 1-diphosphate binding By similarity
	Region	124 – 132	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	26	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	99	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	100	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	103	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	105	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	128	1	Orotate By similarity
	Binding site	156	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BT67-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 3F89FE5BCC66BA26	FASTA	213	23,684
	10 20 30 40 50 60 MEQYKHDFIE FALSRNVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLAKLG EFYAQAIQAS 70 80 90 100 110 120 GLNFDVLFGP AYKGIPIATT VAVALVNQFD VDKPCCFNRK EAKDHGEGGN LIGSPLKGRI 130 140 150 160 170 180 LLVDDVITAG TAIRESMEII NANQAELAGV LIALNRKEKG KGELSAIQEV ERDYGCQVFS 190 200 210 IIDFDDLIQF IEKSEQYAPY LASMRAYREQ YGV

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY68924.1.
RefSeq	YP_001651368.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5851658.
GenomeReviews	Gene locus APJL_0333 in contig CP000687_GR.
KEGG	apj:APJL_0333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YKGITLA.
Family and domain databases
HAMAP	MF_01208. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_ACTPJ

Accession

Primary (citable) accession number: B0BT67

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)