Reviewed, UniProtKB/Swiss-Prot B0BT17 (CYSI_ACTPJ)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	APJL_1881

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_1000146640
Sites
	Metal binding	442	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	448	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	487	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	491	1	Iron (siroheme axial ligand) By similarity
	Metal binding	491	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BT17-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: D5164579B106B883	FASTA	588	66,770
	10 20 30 40 50 60 MSDKKIKGLE WQEKPLSDNE RLKTDSNFLR GTILDDLKDG LTGGFKGDNF QLIRFHGMYE 70 80 90 100 110 120 QDDRDIRAER LEEKLEPLKF MLLRCRLPGG IIKPYQWIEI DKFAREHTRY QSIRLTNRQT 130 140 150 160 170 180 FQYHGVPKGK LQPMHRLLHS IGLDSIATAA DMNRNVLCTS NPIESELHQQ AYEFAKKISE 190 200 210 220 230 240 HLLPRSRGYL DVWVDGKKVE SSDDLLKIED EPILGKTYLP RKFKTAVAIP PLNDVDVYAN 250 260 270 280 290 300 DLNFIAIQDE NGQLCGFNVL VGGGLSFEHG NTKTYPNVAY SLGFVPLEHT LAAAEGVVKT 310 320 330 340 350 360 QRDFGNRSDR KNARVRYTVQ NMTLDGFRAE VERCMNIKFE PTRPYEFTER GDRIGWVKGI 370 380 390 400 410 420 DNNWHLTLFI ESGRITDKPE KPLMTGVLEL AKVHKGDFRI TANQNLIVAN VAEQDKAQIE 430 440 450 460 470 480 AIARQYGLIQ EISKLRENAM SCVSLPTCPL AMAEAERVLP DFISELDKVL SKHNVADESI 490 500 510 520 530 540 ITRITGCPNG CRRAMLAEIG LVGKAIGRYN LHIGGDRAGL RIPRLYKENI TLQEIVNEID 550 560 570 580 QLVARWATER QTNEAFGDFV IRSNIIAPVV NAHIDFWDAT KIIPTTII

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY70431.1.
RefSeq	YP_001652875.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5851546.
GenomeReviews	Gene locus APJL_1881 in contig CP000687_GR.
KEGG	apj:APJL_1881.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ITTTQWQ.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_ACTPJ

Accession

Primary (citable) accession number: B0BT17

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)