B0BT12 (ASNA_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	APJL_1876

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000129105

Sequences

	Sequence		Length	Mass (Da)
	B0BT12 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: CC81BA09B1E079BD	FASTA	330	37,421
	10 20 30 40 50 60 MKKTFILQQQ EISFVKNTFT QYLIDKLGII EVQGPILSQV GNGMQDNLSG IEKAVQVNVK 70 80 90 100 110 120 CIPGATFEVV HSLAKWKRHT LARFGFKEGE GLFVHMKALR PDEDSLDPTH SVYVDQWDWE 130 140 150 160 170 180 KVIPEGRRNF DFLKETVNEI YKAIRLTELA VEARFDIPSI LPKQITFVHS EELVQRYPNM 190 200 210 220 230 240 TGKERENAIC KEHGAVFLIG IGGKLSDGKP HDGRAPDYDD WTTESENGYK GLNGDILVWN 250 260 270 280 290 300 EQLGTAFELS SMGIRVDEKA LRLQVELTGD QDRLEMDWHK DLLAGRLPLS IGGGIGQSRL 310 320 330 VMFLLRKAHI GEVQSSVWPK AMLEKYKNIL

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JL03.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY70426.1.
RefSeq	YP_001652870.1. NC_010278.1.
3D structure databases
ProteinModelPortal	B0BT12.
SMR	B0BT12. Positions 5-330.
ModBase	Search...
Protein-protein interaction databases
STRING	B0BT12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5851450.
GenomeReviews	Gene locus APJL_1876 in contig CP000687_GR.
KEGG	apj:APJL_1876.
PATRIC	20753094. VBIActPle136345_1869.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	APLE434271:APJL_1876-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_ACTPJ

Accession

Primary (citable) accession number: B0BT12

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program