B0BSX2 (SYGA_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	APJL_1836

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000101172

Sequences

	Sequence		Length	Mass (Da)
	B0BSX2 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: F95FD7C89F0EFF8B	FASTA	304	34,670
	10 20 30 40 50 60 MTTKFNVKTF QGMILALQDY WANVGCTIVQ PFDMEVGAGT SHPMTALRAL GPEPMAFAYV 70 80 90 100 110 120 QPSRRPTDGR YGENPNRLQH YYQFQVVIKP SPDNIQELYL GSLKMLGFDP TQHDIRFVED 130 140 150 160 170 180 NWENPTLGAW GLGWEVWLNG MEVTQFTYFQ QVGGLECKPV TGEVTYGLER LAMYIQGVDS 190 200 210 220 230 240 VYDLVWSDGP LGKTTYGDVF HQNEVEQSTY NFEYADVDFL FKAFEQYEKE ATELLALEKP 250 260 270 280 290 300 LPLPAYERIL KAAHSFNMLD ARKAISVTER QRYILRIRTL TKGVAEAYYA SREALGFPGC NKQV

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JL03.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY70386.1.
RefSeq	YP_001652830.1. NC_010278.1.
3D structure databases
ProteinModelPortal	B0BSX2.
SMR	B0BSX2. Positions 10-288.
ModBase	Search...
Protein-protein interaction databases
STRING	B0BSX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5851805.
GenomeReviews	Gene locus APJL_1836 in contig CP000687_GR.
KEGG	apj:APJL_1836.
PATRIC	20753008. VBIActPle136345_1829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	APLE434271:APJL_1836-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_ACTPJ

Accession

Primary (citable) accession number: B0BSX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program