Reviewed, UniProtKB/Swiss-Prot B0BSQ0 (ARGB_ACTPJ)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	APJL_0248

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Acetylglutamate kinase HAMAP MF_00082		PRO_0000335604
Regions
	Region	66 – 67	2	Substrate binding By similarity
Sites
	Binding site	88	1	Substrate By similarity
	Binding site	179	1	Substrate By similarity
	Site	31	1	Transition state stabilizer By similarity
	Site	242	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BSQ0-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: E46750C63501CCE4	FASTA	284	30,305
	10 20 30 40 50 60 MLQNEVENFT NLLEQATVYL APYQEKIIVV KYGGNAMINE DLKKLVMQDI LLLNQLGVKV 70 80 90 100 110 120 VLVHGGGPEI SQGVKLLGKE PQFINGLRVT DQDTINVVLQ MLAGKVNKSL VALLKGKGVG 130 140 150 160 170 180 LCGIDANMLQ CEKLQAEVDY GFVGEIVKVN TQLLELALSA NLIPVISTVG VDDQGVAYNI 190 200 210 220 230 240 NADTVASEIA MALGAAKLVS MTDIAGLLRD RFDESTLIPE VEVSEVQGLI DQGIIAGGMI 250 260 270 280 PKIACCTDFI NAGGIEANII DGRVPHAILV SLFGGKNGTL FYKK

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY68852.1.
RefSeq	YP_001651296.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5851082.
GenomeReviews	Gene locus APJL_0248 in contig CP000687_GR.
KEGG	apj:APJL_0248.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FVIKYGG.
Family and domain databases
HAMAP	MF_00082. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. IPR001057. Glu_5kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_ACTPJ

Accession

Primary (citable) accession number: B0BSQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)