Reviewed, UniProtKB/Swiss-Prot B0BSL0 (DXS_ACTPJ)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	APJL_0208

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115716

Sequences

	Sequence		Length	Mass (Da)
	B0BSL0-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 5573F10785EE1476	FASTA	617	67,842
	10 20 30 40 50 60 MQNKYPLLSQ INSPEDLRLL AKEQLQSVAD ELRAYLLESV SQTSGHLASG LGVVELTVAL 70 80 90 100 110 120 HYVYQTPFDQ LIWDVGHQAY PHKILTGRRD QMHTIRQKNG IHPFPWREES LYDVLSVGHS 130 140 150 160 170 180 STSISAGVGI AVAAEKENAG RKTVCVIGDG AITAGMAFEA MNHAGALHTD MLVILNDNEM 190 200 210 220 230 240 SISENVGALN NHLARIFSGS IYTTVRDGSK KVLDKVPTIK NFMKKSEEHM KGVISPESTL 250 260 270 280 290 300 FEELGFNYIG PIDGHNIDEL VKTLSNMREL KGPQFLHIRT KKGKGYEPAE NDPIGYHGVP 310 320 330 340 350 360 KFDPTCGQLP KSKTIPTYSD IFGNWLCEMA EQDSKLIGIT PAMREGSGMV EFSKRFPEQY 370 380 390 400 410 420 FDVAIAEQHA VTFGAGLAIA GYKPVVAIYS SFLQRAYDQL IHDVAIQNLP VIFAIDRAGI 430 440 450 460 470 480 VGADGQTHQG AFDVSFMRCI PNMTIMCPSD ENEMRQMLYT AYRMNTPTAV RYPRGNAQGV 490 500 510 520 530 540 ALAPMQALEV GKGKLIQQGQ KVAILNFGPL LNEARIVAEK HNYTLADMRF VKPLDEQLVA 550 560 570 580 590 600 ELADSHELLV TLEENAIQGG AGSAVNEYLQ KIGKIRPLVM LGIPDFFVPQ ATQAESYADL 610 GLDAAGIEQR IQAVVNQ

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY68812.1.
RefSeq	YP_001651256.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5850977.
GenomeReviews	Gene locus APJL_0208 in contig CP000687_GR.
KEGG	apj:APJL_0208.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QRFPDRY.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ACTPJ

Accession

Primary (citable) accession number: B0BSL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)