Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi408 – 4081Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi411 – 4111Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciAPLE434271:GIX7-144-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:APJL_0140
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000008547 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4694693-isopropylmalate dehydratase large subunitPRO_1000135660Add
BLAST

Proteomic databases

PRIDEiB0BS44.

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi434271.APJL_0140.

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BS44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTLYEKLF DAHVVYEAAG ETPILYINRH LIHEVTSPQA FDGLRVAGRQ
60 70 80 90 100
VRQIGKTFGT MDHSISTQVR DVNKLEGQAK IQVLELAKNC EASGISLFDM
110 120 130 140 150
QTKEQGIVHV MGPEQGLTLP GMTIVCGDSH TATHGAFGAL AFGIGTSEVE
160 170 180 190 200
HVLATQTLKQ ARAKSMKVEV RGKVNPGITA KDIVLAIIGK TTMAGGTGHV
210 220 230 240 250
VEFYGEAIRD LSMEGRMTVC NMAIEFGAKA GLVAPDETTF AYLKDRPHAP
260 270 280 290 300
KGKDWDDAVE YWKTLKSDDD AVFDSVVVLE AKDIAPQVTW GTNPGQVIGI
310 320 330 340 350
DQVVPNPQEM ADPVTKASAE KALAYIGLDA NTDMKNIPVD QVFIGSCTNS
360 370 380 390 400
RIEDLRAAAA VMKGRKKADN VKRVLVVPGS GLVKEQAEKE GLDKIFIEAG
410 420 430 440 450
AEWRNPGCSM CLGMNDDRLG EWERCASTSN RNFEGRQGRN GRTHLVSPAM
460
AAAAAMFGKF VDIRHVELN
Length:469
Mass (Da):50,766
Last modified:February 26, 2008 - v1
Checksum:iD1EBDC388A2E4FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY68744.1.
RefSeqiYP_001651188.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY68744; ABY68744; APJL_0140.
GeneIDi5850717.
KEGGiapj:APJL_0140.
PATRICi20749489. VBIActPle136345_0141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000687 Genomic DNA. Translation: ABY68744.1.
RefSeqiYP_001651188.1. NC_010278.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi434271.APJL_0140.

Proteomic databases

PRIDEiB0BS44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABY68744; ABY68744; APJL_0140.
GeneIDi5850717.
KEGGiapj:APJL_0140.
PATRICi20749489. VBIActPle136345_0141.

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciAPLE434271:GIX7-144-MONOMER.

Family and domain databases

Gene3Di3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
    Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T.
    , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
    PLoS ONE 3:E1450-E1450(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JL03.

Entry informationi

Entry nameiLEUC_ACTPJ
AccessioniPrimary (citable) accession number: B0BS44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 26, 2008
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.