Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B0BS44 (LEUC_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit

EC=4.2.1.33
Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase
Gene names
Name:leuC
Ordered Locus Names:APJL_0140
OrganismActinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]
Taxonomic identifier434271 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4694693-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026
PRO_1000135660

Sites

Metal binding3471Iron-sulfur (4Fe-4S) By similarity
Metal binding4081Iron-sulfur (4Fe-4S) By similarity
Metal binding4111Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0BS44 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: D1EBDC388A2E4FD3

FASTA46950,766
        10         20         30         40         50         60 
MAKTLYEKLF DAHVVYEAAG ETPILYINRH LIHEVTSPQA FDGLRVAGRQ VRQIGKTFGT 

        70         80         90        100        110        120 
MDHSISTQVR DVNKLEGQAK IQVLELAKNC EASGISLFDM QTKEQGIVHV MGPEQGLTLP 

       130        140        150        160        170        180 
GMTIVCGDSH TATHGAFGAL AFGIGTSEVE HVLATQTLKQ ARAKSMKVEV RGKVNPGITA 

       190        200        210        220        230        240 
KDIVLAIIGK TTMAGGTGHV VEFYGEAIRD LSMEGRMTVC NMAIEFGAKA GLVAPDETTF 

       250        260        270        280        290        300 
AYLKDRPHAP KGKDWDDAVE YWKTLKSDDD AVFDSVVVLE AKDIAPQVTW GTNPGQVIGI 

       310        320        330        340        350        360 
DQVVPNPQEM ADPVTKASAE KALAYIGLDA NTDMKNIPVD QVFIGSCTNS RIEDLRAAAA 

       370        380        390        400        410        420 
VMKGRKKADN VKRVLVVPGS GLVKEQAEKE GLDKIFIEAG AEWRNPGCSM CLGMNDDRLG 

       430        440        450        460 
EWERCASTSN RNFEGRQGRN GRTHLVSPAM AAAAAMFGKF VDIRHVELN 

« Hide

References

[1]"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. expand/collapse author list , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JL03.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000687 Genomic DNA. Translation: ABY68744.1.
RefSeqYP_001651188.1. NC_010278.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING434271.APJL_0140.

Proteomic databases

PRIDEB0BS44.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY68744; ABY68744; APJL_0140.
GeneID5850717.
KEGGapj:APJL_0140.
PATRIC20749489. VBIActPle136345_0141.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0065.
HOGENOMHOG000226972.
KOK01703.
OMADKVWDLH.
OrthoDBEOG600DP5.
ProtClustDBPRK05478.

Enzyme and pathway databases

BioCycAPLE434271:GIX7-144-MONOMER.
UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPMF_01026. LeuC_type1.
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. SSF53732. 1 hit.
TIGRFAMsTIGR00170. leuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEUC_ACTPJ
AccessionPrimary (citable) accession number: B0BS44
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 26, 2008
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways