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B0BS44

- LEUC_ACTPJ

UniProt

B0BS44 - LEUC_ACTPJ

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Protein
3-isopropylmalate dehydratase large subunit
Gene
leuC, APJL_0140
Organism
Actinobacillus pleuropneumoniae serotype 3 (strain JL03)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi408 – 4081Iron-sulfur (4Fe-4S) By similarity
Metal bindingi411 – 4111Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciAPLE434271:GIX7-144-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunit (EC:4.2.1.33)
Alternative name(s):
Alpha-IPM isomerase
Short name:
IPMI
Isopropylmalate isomerase
Gene namesi
Name:leuC
Ordered Locus Names:APJL_0140
OrganismiActinobacillus pleuropneumoniae serotype 3 (strain JL03)
Taxonomic identifieri434271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000008547: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4694693-isopropylmalate dehydratase large subunitUniRule annotation
PRO_1000135660Add
BLAST

Proteomic databases

PRIDEiB0BS44.

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi434271.APJL_0140.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiQARAKTM.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0BS44-1 [UniParc]FASTAAdd to Basket

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MAKTLYEKLF DAHVVYEAAG ETPILYINRH LIHEVTSPQA FDGLRVAGRQ    50
VRQIGKTFGT MDHSISTQVR DVNKLEGQAK IQVLELAKNC EASGISLFDM 100
QTKEQGIVHV MGPEQGLTLP GMTIVCGDSH TATHGAFGAL AFGIGTSEVE 150
HVLATQTLKQ ARAKSMKVEV RGKVNPGITA KDIVLAIIGK TTMAGGTGHV 200
VEFYGEAIRD LSMEGRMTVC NMAIEFGAKA GLVAPDETTF AYLKDRPHAP 250
KGKDWDDAVE YWKTLKSDDD AVFDSVVVLE AKDIAPQVTW GTNPGQVIGI 300
DQVVPNPQEM ADPVTKASAE KALAYIGLDA NTDMKNIPVD QVFIGSCTNS 350
RIEDLRAAAA VMKGRKKADN VKRVLVVPGS GLVKEQAEKE GLDKIFIEAG 400
AEWRNPGCSM CLGMNDDRLG EWERCASTSN RNFEGRQGRN GRTHLVSPAM 450
AAAAAMFGKF VDIRHVELN 469
Length:469
Mass (Da):50,766
Last modified:February 26, 2008 - v1
Checksum:iD1EBDC388A2E4FD3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000687 Genomic DNA. Translation: ABY68744.1.
RefSeqiYP_001651188.1. NC_010278.1.

Genome annotation databases

EnsemblBacteriaiABY68744; ABY68744; APJL_0140.
GeneIDi5850717.
KEGGiapj:APJL_0140.
PATRICi20749489. VBIActPle136345_0141.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000687 Genomic DNA. Translation: ABY68744.1 .
RefSeqi YP_001651188.1. NC_010278.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 434271.APJL_0140.

Proteomic databases

PRIDEi B0BS44.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABY68744 ; ABY68744 ; APJL_0140 .
GeneIDi 5850717.
KEGGi apj:APJL_0140.
PATRICi 20749489. VBIActPle136345_0141.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi QARAKTM.
OrthoDBi EOG600DP5.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci APLE434271:GIX7-144-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
    Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T.
    , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
    PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JL03.

Entry informationi

Entry nameiLEUC_ACTPJ
AccessioniPrimary (citable) accession number: B0BS44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 26, 2008
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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