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B0BS22 (BIOB_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:APJL_0118
OrganismActinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]
Taxonomic identifier434271 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Biotin synthase HAMAP-Rule MF_01694
PRO_0000381182

Sites

Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1131Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1441Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2041Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2761Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0BS22 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: E3CB246F62B10D9F

FASTA33637,629
        10         20         30         40         50         60 
MTTYNLRLKA KSELTPHPTA QYWRKCQVEE LFEMPFMELI FKAAQVHREH FDPQAIQLST 

        70         80         90        100        110        120 
LLSIKTGGCP EECEYCPQSA RYDTGLEKQV MMDVEEIVEK AKIAKARGAS RFCMGAAWRG 

       130        140        150        160        170        180 
PKPKDIKVVS EIIGVVKALG LETCGTFGLL EDGMAEDLKQ AGLDYYNHNL DTDPERYNKI 

       190        200        210        220        230        240 
VHTRNHDDRM DTLGKVRHAG LKVCCGGIVG MNETRPERAG LIASLANLDP QPESVPINQL 

       250        260        270        280        290        300 
IKVEGTPLEN AEDLDWTEFV RTIAVARITM PKSYVRLSAG RTSMPEAMQT LCFMAGANSI 

       310        320        330 
FYGEKLLTTC NPEEDSDRLL MDKLDLYPLA YEPSDA 

« Hide

References

[1]"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. expand/collapse author list , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JL03.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000687 Genomic DNA. Translation: ABY68722.1.
RefSeqYP_001651166.1. NC_010278.1.

3D structure databases

ProteinModelPortalB0BS22.
SMRB0BS22. Positions 23-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING434271.APJL_0118.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY68722; ABY68722; APJL_0118.
GeneID5851552.
KEGGapj:APJL_0118.
PATRIC20749445. VBIActPle136345_0119.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycAPLE434271:GIX7-122-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ACTPJ
AccessionPrimary (citable) accession number: B0BS22
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 26, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways