Reviewed, UniProtKB/Swiss-Prot B0BS03 (ILVD_ACTPJ)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	APJL_0097

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089364
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B0BS03-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 7BEBD1C51958AEB9	FASTA	611	65,408
	10 20 30 40 50 60 MPILRSATST QGRNMAGARA LWRATGMKEN DFGKPIIAVV NSFTQFVPGH VHLRDMGKLV 70 80 90 100 110 120 AEQIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRDLI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPA VFVSGGPMEA GKTKLSNQLI KLDLVDAMMK SADKTVCDDD 190 200 210 220 230 240 VDAIEKSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS MLATHADRKE LFLTAGRQIV 250 260 270 280 290 300 ELCKRYYEQD DASVLPRSIA TKAAFENAMS LDIAMGGSTN TVLHLLAAAQ EAEVDFTMAD 310 320 330 340 350 360 IDRLSRKVPC LSKVAPNTNK YHMEDVHRAG GIMAILGELE RADLLHSDTR TVLGMTIGEQ 370 380 390 400 410 420 IAKYDITLTK DEAVHKFFRA GPAGIRTTKA FSQDCRWDTV DDDRQNGCIR SKEFAYSQDG 430 440 450 460 470 480 GLAMLTGNIA LDGCIVKTAG VDESILKFTG DAIVFESQEE AVEGILGGKV RAGHVVIIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTTYLK SIGLGKECAL LTDGRFSGGT SGLSIGHCSP EAASGGTIGL 550 560 570 580 590 600 VRDGDKIAID IPNRSIQLLV SDEELAVRRA EQDAKGWKPA NRQREVSMAL KMFGHFATSA 610 DKGAVRDKTK L

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY68703.1.
RefSeq	YP_001651147.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5850902.
GenomeReviews	Gene locus APJL_0097 in contig CP000687_GR.
KEGG	apj:APJL_0097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ACTPJ

Accession

Primary (citable) accession number: B0BS03

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)