Reviewed, UniProtKB/Swiss-Prot B0BRY5 (GLPE_ACTPJ)

Names and origin

Protein names

Recommended name:
    Thiosulfate sulfurtransferase glpE
    EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	APJL_0079

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity HAMAP MF_01009.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_1000190094
Regions
	Domain	18 – 106	89	Rhodanese
Sites
	Active site	66	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BRY5-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: E31DA8D21E2E1816	FASTA	108	12,385
	10 20 30 40 50 60 MSETFTEISP HQAWELIENE GATLADIRDG RRYAYSHPQD AFHLTNESYG RFLDEVDYEE 70 80 90 100 PVIVICYHGV SSRNTAQFLV EQGFDRVYSV KGGFDGWERS GLPIETAY

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY68685.1.
RefSeq	YP_001651129.1.
3D structure databases
SMR	B0BRY5. Positions 1-107.
ModBase	Search...
Genome annotation databases
GeneID	5852083.
GenomeReviews	Gene locus APJL_0079 in contig CP000687_GR.
KEGG	apj:APJL_0079.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG743107.
OMA	FSAWHEA.
Family and domain databases
HAMAP	MF_01009. Thiosulf_sulfurtr. [Tree]
InterPro	IPR001763. Rhodanese-like. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_ACTPJ

Accession

Primary (citable) accession number: B0BRY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 26, 2008
Last modified:	January 19, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)