B0BRY0 (RSMG_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.170

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase

Gene names

Name:	rsmG
Ordered Locus Names:	APJL_1687

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › Actinobacillus pleuropneumoniae serovar 3

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanine in position 527 of 16S rRNA By similarity. HAMAP-Rule MF_00074
Catalytic activity	S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA. HAMAP-Rule MF_00074
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00074.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Ribosomal RNA small subunit methyltransferase G HAMAP-Rule MF_00074		PRO_0000342902
Regions
	Region	124 – 125	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	73	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	78	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	138	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BRY0 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 1DBBF4608DA3F3A0	FASTA	205	23,267
	10 20 30 40 50 60 MRQKLDRLLE QAQINLTDQQ KEQLVGFVRL LDKWNKAYNL TSVRNPDEML VKHILDSLVV 70 80 90 100 110 120 SEHLQGNNFI DVGTGPGLPG IPLAIANPDK QFVLLDSLGK RITFIKNALR ELGITNVTPV 130 140 150 160 170 180 LSRVEEYKEQ TFDGVLSRAF ASLNDMVDWC YHLPNPQGKF YALKGIYAES EVQEIKNPIW 190 200 LEKVIPLSVP ELVGERHLVL LNKPN

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JL03.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000687 Genomic DNA. Translation: ABY70239.1.
RefSeq	YP_001652683.1. NC_010278.1.
3D structure databases
ProteinModelPortal	B0BRY0.
ModBase	Search...
Protein-protein interaction databases
STRING	434271.APJL_1687.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY70239; ABY70239; APJL_1687.
GeneID	5851079.
KEGG	apj:APJL_1687.
PATRIC	20752699. VBIActPle136345_1679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0357.
HOGENOM	HOG000221012.
KO	K03501.
OMA	DSHFTLL.
ProtClustDB	PRK00107.
Enzyme and pathway databases
BioCyc	APLE434271:GIX7-1727-MONOMER.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G.
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. gidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_ACTPJ

Accession

Primary (citable) accession number: B0BRY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program