Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B0BRY0 (RSMG_ACTPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase G

EC=2.1.1.170
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase
Gene names
Name:rsmG
Ordered Locus Names:APJL_1687
OrganismActinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]
Taxonomic identifier434271 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length205 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA By similarity. HAMAP-Rule MF_00074

Catalytic activity

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA. HAMAP-Rule MF_00074

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00074.

Sequence similarities

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionrRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 205205Ribosomal RNA small subunit methyltransferase G HAMAP-Rule MF_00074
PRO_0000342902

Regions

Region124 – 1252S-adenosyl-L-methionine binding By similarity

Sites

Binding site731S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site781S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1381S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
B0BRY0 [UniParc].

Last modified February 26, 2008. Version 1.
Checksum: 1DBBF4608DA3F3A0

FASTA20523,267
        10         20         30         40         50         60 
MRQKLDRLLE QAQINLTDQQ KEQLVGFVRL LDKWNKAYNL TSVRNPDEML VKHILDSLVV 

        70         80         90        100        110        120 
SEHLQGNNFI DVGTGPGLPG IPLAIANPDK QFVLLDSLGK RITFIKNALR ELGITNVTPV 

       130        140        150        160        170        180 
LSRVEEYKEQ TFDGVLSRAF ASLNDMVDWC YHLPNPQGKF YALKGIYAES EVQEIKNPIW 

       190        200 
LEKVIPLSVP ELVGERHLVL LNKPN 

« Hide

References

[1]"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China."
Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. expand/collapse author list , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H.
PLoS ONE 3:E1450-E1450(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JL03.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000687 Genomic DNA. Translation: ABY70239.1.
RefSeqYP_001652683.1. NC_010278.1.

3D structure databases

ProteinModelPortalB0BRY0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING434271.APJL_1687.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABY70239; ABY70239; APJL_1687.
GeneID5851079.
KEGGapj:APJL_1687.
PATRIC20752699. VBIActPle136345_1679.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0357.
HOGENOMHOG000221012.
KOK03501.
OMAKLDNITP.
OrthoDBEOG6HF639.

Enzyme and pathway databases

BioCycAPLE434271:GIX7-1727-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_00074. 16SrRNA_methyltr_G.
InterProIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02527. GidB. 1 hit.
[Graphical view]
PIRSFPIRSF003078. GidB. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00138. gidB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRSMG_ACTPJ
AccessionPrimary (citable) accession number: B0BRY0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: February 26, 2008
Last modified: June 11, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families