Reviewed, UniProtKB/Swiss-Prot B0BRH9 (DDL_ACTPJ)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	APJL_0021

Organism

Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP]

Taxonomic identifier

434271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	D-alanine--D-alanine ligase HAMAP MF_00047		PRO_0000341047
Regions
	Domain	104 – 300	197	ATP-grasp
	Nucleotide binding	132 – 187	56	ATP By similarity
Sites
	Metal binding	254	1	Magnesium or manganese 1 By similarity
	Metal binding	267	1	Magnesium or manganese 1 By similarity
	Metal binding	267	1	Magnesium or manganese 2 By similarity
	Metal binding	269	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0BRH9-1 [UniParc]. Last modified February 26, 2008. Version 1. Checksum: 620151A5C69B3D2E	FASTA	303	32,704
	10 20 30 40 50 60 MSIKDEKIAV LFGGVSQERE VSLNSGAAVT EALKSLGYNV EGIDTKDFPI EKLKEKGIQR 70 80 90 100 110 120 VFNILHGGIG ENGVLQGALE QMGIPYTGCG VMASAVTLDK FRTKLMWQAV GLPTADMVVV 130 140 150 160 170 180 RRGEAVDSEQ IIAKLGLPVF VKPSSEGSSV GVTKVKTVEQ LLPAVEEALK FDSIVLVEAF 190 200 210 220 230 240 LAGKEYSVPV LDGQVLPAVQ IIPEGEFYDY HAKYISDNTQ YLVPALSDDR QAEVAELVKA 250 260 270 280 290 300 AYEVVGCRGW SRIDVMEDAN GHFNLVEVNT CPGMTSHSIF PKSAATVGIP FEKLVERVLE LSA

References

[1]

"Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. , Zheng H., Pu S., Wang B., Gu W., Zhang X.L., Zhu G.-F., Wang S., Zhao G.-P., Chen H. PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000687 Genomic DNA. Translation: ABY68627.1.
RefSeq	YP_001651071.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5851576.
GenomeReviews	Gene locus APJL_0021 in contig CP000687_GR.
KEGG	apj:APJL_0021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0BRH9. RAFIILH.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ACTPJ

Accession

Primary (citable) accession number: B0BRH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 26, 2008
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)