Reviewed,
UniProtKB/Swiss-Prot B0BRH9 (DDL_ACTPJ)
Last modified
November 25, 2008.
Version 10.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: D-alanine--D-alanine ligase EC=6.3.2.4 Alternative name(s): D-alanylalanine synthetase D-Ala-D-Ala ligase | ||||
| Gene names |
| ||||
| Organism | Actinobacillus pleuropneumoniae serotype 3 (strain JL03) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 434271 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus |
Protein attributes
| Sequence length | 303 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Cell wall formation By similarity. |
| Catalytic activity | ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. |
| Cofactor | Binds 2 magnesium or manganese ions per subunit By similarity. |
| Pathway | |
| Subcellular location | CytoplasmBy similarity. |
| Sequence similarities | Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shapeInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cell wall Inferred from electronic annotation. Source: InterPro cytoplasmInferred from electronic annotation. Source: HAMAP |
| Molecular function | ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activityInferred from electronic annotation. Source: HAMAP magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 303 | 303 | D-alanine--D-alanine ligase | PRO_0000341047 | |||||
Regions | |||||||||
| Domain | 104 – 300 | 197 | ATP-grasp | ||||||
| Nucleotide binding | 132 – 187 | 56 | ATP By similarity | ||||||
Sites | |||||||||
| Metal binding | 254 | 1 | Magnesium or manganese 1 By similarity | ||||||
| Metal binding | 267 | 1 | Magnesium or manganese 1 By similarity | ||||||
| Metal binding | 267 | 1 | Magnesium or manganese 2 By similarity | ||||||
| Metal binding | 269 | 1 | Magnesium or manganese 2 By similarity | ||||||
Sequences
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References
| [1] | "Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China." Xu Z., Zhou Y., Li L., Zhou R., Xiao S., Wan Y., Zhang S., Wang K., Li W., Li L., Jin H., Kang M., Dalai B., Li T., Liu L., Cheng Y., Zhang L., Xu T. Chen H.PLoS ONE 3:E1450-E1450(2008) [PubMed: 18197260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| CP000687 Genomic DNA. Translation: ABY68627.1. | |
| RefSeq | YP_001651071.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 5851576. |
| GenomeReviews | Gene locus APJL_0021 in contig CP000687_GR. |
| KEGG | apj:APJL_0021. |
Organism-specific databases | |
| CMR | Search... |
Family and domain databases | |
| HAMAP | MF_00047. [Tree] |
| InterPro | IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view] |
| Gene3D | G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01205. D_ala_D_alaTIGR. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DDL_ACTPJ | ||||||||
| Accession | Primary (citable) accession number: B0BRH9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


